[논문]복령과 황태껍질 발효물 혼합물의 고지방식이 유도 비만 동물모델에서의 항비만효과

김미자; 강순아

doi:10.9799/ksfan.2023.36.5.403

복령과 황태껍질 발효물 혼합물의 고지방식이 유도 비만 동물모델에서의 항비만효과
Anti-Obesity Effect of Fermented Pollack Skin and Wolfiporia extensa Ginns in High Fat-induced Obesity Animal Model 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.5, 2023년, pp.403 - 414

김미자 (호서대학교 보건산업연구소) , 강순아 (호서대학교 벤처대학원 융합공학과)

Abstract ▼ AI-Helper

This study aimed to determine the anti-obesity effect of adding Wolfiporia extensa Ginns (W) to fermented pollack skin products in an obesity-induced animal model. The experimental groups were the normal diet group (C), high-fat diet group (HF), dried pollack skin (H1), fermented pollack skin (H2), and W of 0.1 (F2-WL), 0.3 (F2-WM), and 0.5 (F2), respectively. It was confirmed that adding W to fermented pollack skin reduced blood triglycerides, total cholesterol, and LDL levels, while increasing HDL levels. Wolfiporia extensa Ginns was effective in controlling weight and improving blood lipids in a dose-dependent manner. In histological analysis, findings of fatty liver induced by a high-fat diet were improved by the addition of H2 and W. Size and density of fat globules in the epididymis were decreased. In addition, the concentration of TNF-α was increased in the high-fat diet group, but decreased by the addition of fermented pollack skin and W. In conclusion, adding fermented dried pollack skin and Wolfiporia extensa Ginns was effective for weight control and blood lipid improvement. Thus, the use of by-products in functional foods is expected to have a high value in the future.

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표/그림 (10)

표 Table 1. Composition of experimental diets (d/kg diet)
그림 Fig. 1. Experimental design.
그림 Fig. 2. Change of body weight calorie intake among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ¹⁾ C: control, DIO: high-fat diet induced obesity animal, HF: high–fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 3. Weight gain and food efficiency ratio among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ^a-cMeans indicated with different letters are significantly different among DIO groups at p<0.05 by Duncan's multiple range test. C: control, DIO: high-fat diet induced obesity animal, HF: high-fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 4. Organ weight changes among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ^a-cMeans indicated with different letters are significantly different among DIO groups at p<0.05 by Duncan's multiple range test. C: control, DIO: high-fat diet induced obesity animal, HF: high-fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 5. Fat tissue weight changes among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ^a-cMeans indicated with different letters are significantly different among DIO groups at p<0.05 by Duncan's multiple range test. C: control, DIO: high-fat diet induced obesity animal, HF: high-fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 6. Blood lipid profiles changes among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ^a-cMeans indicated with different letters are significantly different among DIO groups at p<0.05 by Duncan's multiple range test. C: control, DIO: high-fat diet induced obesity animal, HF: high-fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 7. Blood GOT, GPT, blood urea nitrogen and creatinine changes among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ^a-cMeans indicated with different letters are significantly different among DIO groups at p<0.05 by Duncan's multiple range test. C: control, DIO: high-fat diet induced obesity animal, HF: high-fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 8. Blood TNF-alpha changes among experimental groups. Each value represents the mean±S.D. *Significantly different from CON vs DIO (p<0.05). ^a-cMeans indicated with different letters are significantly different among DIO groups at p<0.05 by Duncan's multiple range test. C: control, DIO: high-fat diet induced obesity animal, HF: high- fat diet, F1: HF + pollack skin, F2: HF + fermented pollack skin, F2_WL: fermented pollack skin + 0.1% Wolfiporia extensa Ginns, F2_WM: fermented pollack skin + 0.3% Wolfiporia extensa Ginns, F2_WH: fermented pollack skin + 0.5% Wolfiporia extensa Ginns.
그림 Fig. 9. Histological change of epididymal fat tissue (×200) by H&E stained (A), hepatic tissue (×200) by Oil Red O staining. (A) H&E staining of epididymal fat showed that HFD signifcantly induced adipocyte hypertrophy, and the size of adipocytes in tissue was signifcantly smaller in F2-WH group than that in HF group. (B) Oil Red O staining results showed remarkable deposition of lipid droplets within the liver in HF mice. As fat accumulates due to a high-fat diet, the density of fat increases and it becomes dyed red. Arrows point to lipid droplets.

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