[논문]Re-Identification of Aspergillus Subgenus Circumdati Strains in Korea Led to the Discovery of Three Unrecorded Species

Anbazhagan Mageswari; Yunhee Choi; Le Dinh Thao; Daseul Lee; Dong-Hyun Kim; Myung Soo Park; Seung-Beom Hong

doi:10.1080/12298093.2023.2257997

Re-Identification of Aspergillus Subgenus Circumdati Strains in Korea Led to the Discovery of Three Unrecorded Species 원문보기

Mycobiology, v.51 no.5, 2023년, pp.288 - 299

Anbazhagan Mageswari (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) , Yunhee Choi (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) , Le Dinh Thao (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) , Daseul Lee (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) , Dong-Hyun Kim (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) , Myung Soo Park (Department of Crops and Forestry, Korea National University of Agriculture and Fisheries) , Seung-Beom Hong (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA)

Abstract ▼ AI-Helper

Aspergillus is one of the largest and diverse genera of fungi with huge economical, biotechnological, and social significance. Taxonomically, Aspergillus is divided into six subgenera comprising 27 sections. In this study, 235 strains of Aspergillus subgenus Circumdati (section: Candidi, Circumdati, Flavi, Flavipedes, Nigri, and Terrei) preserved at the Korean Agricultural Culture Collection (KACC) were analyzed and re-identified using a combined dataset of partial b-tubulin (BenA), Calmodulin (CaM) gene sequences and morphological data. We confirmed nineteen species to be priorly reported in Korea (A. neotritici, A. terreus, A. floccosus, A. allahabadii, A. steynii, A. westerdijkiae, A. ochraceus, A. ostianus, A. sclerotiorum, A. luchuensis, A. tubingensis, A. niger, A. welwitschiae, A. japonicus, A. nomius, A. tamarii, A. parasiticus, A. flavi, and A. oryzae). Among the studied strains, three species (A. subalbidus, A. iizukae, and A. uvarum), previously unreported or not officially documented, were discovered in Korea, to the best of our knowledge. We have given a detailed description of the characteristic features of the three species, which remain uncharted in Korea.

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표/그림 (8)

표 Table 1. Aspergillus subgenus Circumdati strains used in this study.
표 Table 1. Continued.
표 Table 2. Reference sequences of Aspergillus species used in the phylogenetic analyses.
그림 Figure 1. Phylogenetic position of Aspergillus subgenus Circumdati strains from the KACC based on a combined data set of partial BenA and CaM sequences. Bootstrap values >50 are presented at the nodes. The unrecorded species are represented in bold and red in color. Ex-type strains are denoted by the symbol "T." A. calidoustus was used as the outgroup.
그림 Figure 1. Continued
그림 Figure 2. Morphology of Aspergillus iizukae (KACC 43789). (A-D) Colonies grown on MEA, CYA, DG18, and YES media after 7 d at 25 ºC from left to right. (E) Conidial heads on MEA. (F & G) Conidiophores with conidial head. (H) Conidia. Scale bars: E = 100 μm, F = 20 μm, G, H = 10 μm.
그림 Figure 3. Morphology of Aspergillus subalbidus (KACC 46482). (A-D) Colonies grown on MEA, CYA, DG18, and YES media after 7 d at 25 ºC from left to right. (E) Conidial head on MEA. (F & G) Conidiophores with conidial head. (H) Conidia. Scale bars: E = 100 μm, F, G = 20 μm, H = 10 μm.
그림 Figure 4. Morphology of Aspergillus uvarum (KACC 48630). (A-D) Colonies grown on MEA, CYA, DG18, and YES media after 7 d at 25 ºC from left to right. (E) Conidial head on MEA. (F & G) Conidiophores with conidial head. (H) Conidia. Scale bars: E = 100 μm, F= 50 μm, G = 20 μm, H = 5 μm.

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