[논문]인간유래각질형성세포에서 호장근 추출물이 피부장벽 보호능과 보습능에 미치는 영향

강은정; 박지아; 최윤식

doi:10.12925/jkocs.2023.40.5.965

인간유래각질형성세포에서 호장근 추출물이 피부장벽 보호능과 보습능에 미치는 영향
The effects of the Reynoutria japonica on skin-barrier and moisturizing in HaCaT cells 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.40 no.5, 2023년, pp.965 - 976

강은정 (경성대학교 약학과) , 박지아 (경성대학교 제약공학과) , 최윤식 (경성대학교 약학과)

초록
AI-Helper

호장은 마디풀과에 속하는 다년초로 우리나라를 포함한 동아시아에서 잘 자생한다. 호장근은 호장의 뿌리로 항염증과 진경제로 사용되어 왔으며 유효성분으로 emodin을 포함하고 있다. 피부의 표피는 자극, 해로운 물질을 차단하고 수분 증발을 방지함으로써 체내를 보호하는 중요한 역할을 한다. 본 연구에서는 호장근과 그 유효성분인 emodin이 피부 장벽과 보습능에 미치는 영향에 대해 평가하고자 하였다. 먼저 호장근은 ABTS⁺ radicals을 우수하게 제거함으로써 항산화 효능이 뛰어남을 확인하였다. 다음으로, 실시간 중합연쇄효소반응을 통해 각질형성세포의 분화에 중요한 역할을 하는 filaggrin의 유전자 발현을 비교한 결과, 호장근과 emodin에 의해 농도-의존적으로 filaggrin mRNA 발현이 증가하였다. 또한, 호장근과 emodin은 히알루론산 합성에 중요한 역할을 하는 HAS-2 mRNA 발현을 유의하게 증가시키는 것으로 나타났다. 종합적으로, emodin을 유효성분으로 포함하는 호장근은 피부 장벽 강화와 보습능 증강을 위한 기능성 화장품 소재로서 활용될 수 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Reynoutria japonica is a perennate plant belonging to Polygonaceae and grows wild in East Asia containing Korea. Roots of Reynoutria japonica (R. japonica), part of roots of Reynoutria japonica, has been used for anti-inflammation and antispasmodics and contains emodin as active compound. Epidermis of skin is crucial roles to defense our body against stimulants, harmful substance and prevent water loss. In this study, we examined the effect of R. japonica and emodin, its active compound, on skin-barrier and moisturizing on HaCaT cells. First, antioxidant effect of R. japonica was prominent by scavenging ABTS+ radicals. Next, we conducted real time PCR and expression of filaggrin mRNA which is crucial role in differentiation of keratinocyte increased by R. japonica and emodin dose-dependently. In addition, R. japonica and emodin significantly elevated the expression of HAS-2 mRNA which play a role in hyaluronic acid synthesis on HaCaT cells. Taken together, R. japonica containing emodin, as active compound has potential as a cosmetic material for enhancing the function of skin-barrier and moisturizing in epidermis.

주제어

표/그림 (6)

표 Table 1. Primer sequence
그림 Fig. 1. HPLC analysis. (A) Calibration curve of emodin (B) Emodin (C) Reynoutria japonica
그림 Fig. 2. Antioxidant effect of Reynoutria japonica. ABTS⁺ radical dose-dependently decreased by Reynoutria japonica. A.A; ascorbic acid
그림 Fig. 3. Effect of Reynoutria japonica on cell viability and toxicity in HaCaT cells. Cell viability was measured by MTT assay and significantly decreased by Reynoutria japonica (A) and emodin (B) dose-dependently. Cell toxicity was determinded by LDH release assay and significantly incrased by Reynoutria japonica (C) and emodin (D) dose-dependently. *p<0.05, ***p<0.001 compared to control.
그림 Fig. 4. Effects of Reynoutria japonica and emodin on filaggrin mRNA expression in HaCaT cells. Filaggrin mRNA significantly increased by Reynoutria japonica (A) and emodin (B) dose-dependently for 4 hr incubation. *p<0.05, ***p<0.001 compared to control. EGCG; (-)-Epigallocatechin Gallate.
그림 Fig. 5. Effects of Reynoutria japonica and emodin on HAS-2 mRNA expression in HaCaT cells. HAS-2 mRNA significantly increased by Reynoutria japonica (A) and emodin (B) dose-dependently for 4 hr incubation. *p<0.05, **p<0.01, ***p<0.001 compared to control; #p<0.05 compared to EGCG.

참고문헌 (36)

E. J. Kang, Y. Jang, J. Lee, S. H. Kim, S.？Kim, J. Bak, Y. Choi, "The Effects of？Chamaecyparis obtusa Oil on Anti-Wrinkle,？Skin-Barrier and Moisturizing", Journal of？the Korean Applied Science and？Technology, Vol.40, No.2 pp. 309-321,？(2023).
H. B. Kang, Y. Choi, J. Bak, Y. Jang,？"Effect of Lysimachia christinae Hance？extract on moisturizing functionand？protection of skin barrier", The Korean？Society of Culture and Convergence, Vol.42,？No.12 pp. 941-958, (2020).
M. Kim, "Skin Barrier and Protein", Journal？of Skin Barrier Research, Vol.11, No.1 pp.？28-34, (2009).
H. Y. Yu, I. J. Yang, V. R. Lincha, I. S.？Park, D. Lee, H. M. Shin, "The Effects of？the Fruits of Foeniculum vulgare on Skin？Barrier Function and Hyaluronic Acid？Production in HaCaT Keratinocytes",？Journal of Life Science, Vol.25, No.8 pp. 880-888, (2015).
Hwang J. 화장품과 피부과학 연구동향. pp.？43-49, Korea Health Industry Development？Institute, (2001).
J. S. Oh, H. H. Jang, "Epidermal？Differentiation and Skin Barrier", Korean？Journal of Aesthetics and Cosmetology, Vol.13, No.6 pp. 713-720, (2015).
M. Hashimoto, K. Maeda, "New Functions？of Low-Molecular-Weight Hyaluronic Acid on Epidermis Filaggrin Production and？Degradation", Cosmetics, Vol.8, No.4 pp.？118, (2021).
E. Kim, Y. Moon, Y. Jang, "Skin？Moisturizing Properties and Anti-Inflammatory effects of extracts from Coptis？chinensis in HaCaT cells", Journal of the？Korean Applied Science and Technology, Vol.38, No.3 pp. 870-888, (2021).
S. Park, S. Ho, D. Kim, Y. Shin, J. O. Lee,？Y. Kim, J. M. Lee, Y Jang, "Effects of？PB203 on the Skin Photoaging of Ultraviolet？B (UVB)-irradiated Hairless Mice and？Human keratinocytes", Journal of？Biomedical and Translational Research, Vol.23, No.4 pp. 215-234, (2022).
X. Wang, L. Ye, Q. Lai, S. Wen, Z. Long,？X. Qiu, P. M. Elias, B. Yang, M. Man,？"Altered Epidermal Permeability Barrier？Function in the Uninvolved Skin Supports a Role of Epidermal Dysfunction in the？Pathogenesis of Occupational Hand Eczema",？Skin Pharmacology and Physiology, Vol.33,？No.2 pp.94-101, (2020).

상세보기
F. Reno, V. Rocchetti, M. Migliario, M.？Rizzi, M. Cannas, "Chronic Exposure to？Cigarette Smoke Increases Matrix？metalloproteinases and Filaggrin mRNA？Expression in Oral keratinocytes: role of？nicotine stimulation", Journal of Oral？Oncology, Vol.47, No.9 pp. 827-830,？(2011).
G. M. O'Regan, A. Sandilands, W. H. I.？McLean, A. D. Irvine, "Filaggrin in Atopic？Dermatitis", Journal of Allergy and Clinical？Immunology, Vol.122, pp. 689-693, (2008).

상세보기
M. H. Kwon, K. Min, Y. Kim, "Inhibitory？Effects of Peonia japonica Water Extract on Skin Aging (I) - Focussed on Alleviative？Effects of Inflammation and Skin Barrier？Damage -". The Korean Society of？Environmental Health and Toxicology, Vol.24, No.2 pp. 159-167, (2009).
S. H. Kim, "A Study on the Effects of Skin？Barrier Damage on TEWL and the Efficacy？of Jojoba oil in Skin Barrier Restitution",？Korean Journal of Aesthetics and？Cosmetology, Vol.3, No.1 pp. 189-200,？(2005).
S. B. Kwon, G. T. Lee, S. J. Choi, N. K.？Lee, H. W. Park, K. S. Lee, K. K. Lee, K. J.？Ahn, I. S. An, "The Effect of Glycerin,？Hyaluronic Acid and Silicone Oil on the？Hydration, Moisturization and？Transepidermal Water Loss in Human？Skin", Korean Journal of Aesthetics and？Cosmetology, Vol.11, No.4 pp. 761-768,？(2013).
E. H. Choi, N. Y. Yoon, "Pathogenesis of？Atopic Dermatitis", Journal of the Korean？Medical Association, Vol.57, No.3 pp.？218-225, (2014).
M. J. Kim, B. S. Hwang, Y. Hwang, Y. T.？Jeong, D. W. Jeong, Y. T. Oh, "Anti-Inflammatory and Antiatopic Effects of？Rorippa cantoniensis (Lour.) Ohwi in RAW 264.7 and HaCaT Cells", Molecules, Vol.28, No.14 pp. 5463, (2023).

상세보기
R. Wang, S. Yan, X. Ma, J. Zhao, Y. Han,？Y. Pan, H. Zhao, "The Pivotal Role of？Bifida Ferment Lysate on Reinforcing the？Skin Barrier Function and Maintaining？Homeostasis of Skin Defenses in vitro",？Journal of Cosmetic Dermatology, doi:10.1111/jocd.15831, (2023).

상세보기
S. Park, I. Jo, S. Park, M. Park, Y. Mun, "Poria cocos Extract from Mushrooms？Stimulates Aquaporin-3 via the PI3K/Akt/mTOR Signaling Pathway", Clinical,？Cosmetic and Investigational Dermatology, Vol.15, pp. 1919-1931, (2022).

상세보기
Y. Yoshino, K. Marunaka, M. Kobayashi,？H. Matsunaga, S. Shu, T. Matsunaga, A.？Ikari, "Protective Effects of Ethanol Extract？of Brazilian Green Propolis and Apigenin？against Weak Ultraviolet Ray-B-Induced？Barrier Dysfunction via Suppressing Nitric？Oxide Production and Mislocalization of？Claudin-1 in HaCaT Cells", International？Journal of Molecular Sciences, Vol.22,？No.19 pp. 10326, (2021).
K. Yang, Y. Sung, H. K. Kim,？"Antithrombotic Effect and Antiplatelet？Activity of Polygonum cuspidatum Extract",？The Korean Society of Food Science and？Nutrition, Vol.41, No.2 pp. 168-173,？(2012).
M. Kim, M. Ryu. "Inhibition of？Melanogenesis and Anti-UV properties？Reynoutria elliptica", Korean Journal of？Aesthetics and Cosmetology, Vol.10, No.4？pp.961-968, (2012).
A. Lim, Y. Jung, K. Kim, Y. Kim, J. Kwak,？J. Hong, H. Y. Kim, D. Kim, "Skin UVB？Photo Aging Effect from Extract of？Fermented Reynoutria elliptica", The Korean？Society of Food Science and Nutrition, Vol.39, No.3 pp. 369-375, (2010).
J. Chang, H. Liu, K. Wang, M. Chen, L.？Chiang, Y. Hua, C. Lin, "Ethanol Extract？of Polygonum cuspidatum Inhibits Hepatitis？B virus in a Stable HBV-producing Cell？line", Antiviral Research, Vol.66, pp. 29-34,？(2005).

상세보기
J. Hwang, Y. Park, Y. Kim, J. Kim, C. Lim,？"Isolation and Identification of Antifungal？Compounds from Reynoutria elliptica",？Journal of Agricultural Science, Vol.39,？No.4 pp. 583-589, (2012).
B. R. Kim, J. Ha, S. Lee, J. Park, S. Cho,？"Anti-cancer Effects of Ethanol Extract of？Reynoutria japonica Houtt. radix in Human？Hepatocellular Carcinoma Cells via？Inhibition of MAPK and PI3K/Akt Signaling？Pathways", Journal of Ethnopharmacology, Vol.245, pp. 112-179, (2019).
H. Jung, S. Roh, M. Oh, "The Effects of？Polygonum Cuspidatum on the Skin？Functions", Journal of Korean Medicine？Rehabilitation, Vol.19, No.1 pp. 73-89,？(2009).
H. Zhang, C. Li, S. Kwok, Q. Zhang, S.？Chan, "A Review of the Pharmacological？Effects of the Dried Root of Polygonum？cuspidatum (Hu Zhang) and Its？Constituents", Evidence-Based Complementary？and Alternative Medicine, Vol.2013, pp. 13,？(2013).

상세보기
E. Kim, J. Kim, H. K. Kim, Y. Jang？"Antimicrobial and Anti-inflammatory？Effects of the Ethanol Extract of Polygonum？cuspidatum as a Mouthwash Component",？Natural Product Communications, Vol.16,？No.4 doi:10.1177/1934578X211005255,？(2021).

상세보기
T. Lee, J. Kim, J. So, "Emodin from？Polygonum cuspidatum showed Angiogenesis？Inhibiting Activity in vitro", The Korean？Society for Applied Biological Chemistry, Vol.46, No.1 pp. 50-54, (2003).
C. Hsu, Y. Chan, J. Chang, "Antioxidant？Activity of Extract from Polygonum？cuspidatum", Biological Research, Vol.40,？No.1 pp. 13-21, (2007).
J. Bak, H. J. Kim, S. Y. Kim, Y. Choi,？"Neuroprotective Effect of Caffeic acid？Phenethyl Ester in 3-nitropropionic acid-induced Striatal Neurotoxicity", The Korean？Journal of Physiology and Pharmacology, Vol.20, No.3 pp. 279-286, (2016).

원문보기 상세보기
S. Kim, J. Choi, Y. Jang, "Emodin Studies on Anti-inflammatory and Skin？Improvement Activities", Journal of the？Korean Applied Science and Technology, Vol.38, No.6 pp. 1383-1392, (2021).
S. M. Ahn, H. N. Kim, Y. R. Kim, Y. W.？Choi, C. M. Kim, H. K. Shin, B. T. Choi,？"Emodin from Polygonum multiflorum ameliorates oxidative toxicity in HT22 cells？and deficits in photothrombotic ischemia",？Journal of Ethnopharmacology, Vol.188, pp.？13-20, (2016).

상세보기
I. Y. Hwang, C. S. Jeong, "A Study on the？Antigastritic Effects of Rheum Species？Extracts and Their Active Components",？Journal of Food Hygiene Safety, Vol.28,？No.4 pp. 330-336, (2013).
K. J. Lee, W. Jang, Y. A. Kim, B. J. Park,？H. Kang, "Antioxidant, Inhibitory on NO？Production and In-vitro Cell Regeneration？Effects of Pink-aloe", Journal of the Society？of Cosmetic Scientists of Korea, Vol.46,？No.3 pp. 273-282, (2020).

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증