[논문]생쥐 대장 카할세포에서 가미소요산, 반하사심탕 및 보중익기탕의 효과에 관한 비교연구

최나리; 최우균; 김병주

doi:10.14374/hfs.2024.32.1.29

[국내논문] 생쥐 대장 카할세포에서 가미소요산, 반하사심탕 및 보중익기탕의 효과에 관한 비교연구
Effects of Gamisoyo-san, Banhasasim-tang and Bojungikki-tang in Colonic Interstitial cells of Cajal in mice

大韓韓醫學方劑學會誌 = Herbal formula science, v.32 no.1, 2024년, pp.29 - 37

최나리 (부산대학교 한의학전문대학원 양생기능의학교실) , 최우균 (부산대학교 한의학전문대학원 양생기능의학교실) , 김병주 (부산대학교 한의학전문대학원 양생기능의학교실)

Abstract ▼ AI-Helper

Objectives : The purpose of this study was to examine the effects of insurance herbal medicines on colonic interstitial Cells of Cajal (ICC) in mice. Methods : Among the insurance herbal medicines, we chose Gamisoyo-san (GSS), Banhasasim-tang (BHSST) and Bojungikki-tang (BGIKT). We made the ICC culture in large intestine in mice and used the electrophysiological method to record pacemaker potentials. Also we used MTT assay to check cell viability and examined the ICC protein expression by western blot. Results : 1. GSS (1-10 mg/ml) induced the pacemaker potential depolarization and decreased frequency with concentration-dependent manners in colonic ICC. EC50 is 2.99 mg/ml. BHSST (1-10 mg/ml) induced the pacemaker potential depolarization and decreased frequency with concentration-dependent manners in colonic ICC. EC50 is 2.76 mg/ml. BGIKT (1-10 mg/ml) induced the pacemaker potential depolarization and decreased frequency with concentration-dependent manners in colonic ICC. EC50 is 4.49 mg/ml. 2. GSS, BHSST and BGIKT had no effects on cell viability in colonic ICC. 3. GSS and BGIKT increased the Anoctamin-1 (ANO1) protein expression and BHSST increased the transient receptor potential melastatin-subfamily member 7 (TRPM7) protein expression in colonic ICC. Conclusions : These results suggest that GSS, BHSST, and BGIKT have shown the potential to regulate gastrointestinal (GI) motility by regulating colonic ICC and may show the potential to treat colon-derived GI diseases such as irritable bowel syndrome (IBS).

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표/그림 (3)

그림 Fig. 1. Effects of GSS, BHSST and BGIKT in pacemaker potentials of murine colonic intestinal ICC. (A) a) GSS depolarized the pacemaker potential and decreased the frequency in colonic intestinal ICC. b) Summary of changes. (B) a) BHSST depolarized the pacemaker potential and decreased the frequency in colonic intestinal ICC. b) Summary of changes. (C) a) BGIKT depolarized the pacemaker potential and decreased the frequency in colonic intestinal ICC. b) Summary of changes. GSS, Gamisoyo-san, BHSST, Banhasasim-tang, BGIKT, Bojungikki-tang.
그림 Fig. 2. GSS, BHSST and BGIKT has no effect on murine colonic ICC viability after 24 h. (A) GSS, (B) BHSST or (C) BGIKT has no effect on murine colonic ICC viability. Results are presented as the mean ± SEM. GSS, Gamisoyo-san, BHSST, Banhasasim-tang, BGIKT, Bojungikki-tang.
그림 Fig. 3. Effects of GSS, BHSST and BGIKT on the expression of ANO1, c-kit and TRPM7 in murine colonic ICC. (A) The expression of ANO1 increased considerably but that of c-kit and TRPM7 was almost unchanged by GSS. (B) The expression of TRPM7 increased considerably but that of ANO1 and c-kit was almost unchanged by BHSST. (C) The expression of ANO1 increased considerably but that of c-kit and TRPM7 was almost unchanged by BGIKT. Results represent the mean ± SEM. *P<0.05. CTRL, control; ANO1, Anoctamin1; TRPM7, transient receptor potential melastatin 7. GSS, Gamisoyo-san, BHSST, Banhasasim-tang, BGIKT, Bojungikki-tang.

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