[논문]흑진미(Oryza sativa cv. Heugjinmi) 추출물의 항산화 및 미백 효능 연구

이주성; 최은영

doi:10.15230/scsk.2024.50.2.131

[국내논문] 흑진미(Oryza sativa cv. Heugjinmi) 추출물의 항산화 및 미백 효능 연구
Anti-oxidant and Whitening Effects of Oryza sativa cv. Heugjinmi Extracts

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.50 no.2, 2024년, pp.131 - 141

이주성 (대구한의대학교 화장품약리학과) , 최은영 (대구한의대학교 화장품약리학과)

초록
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본 연구에서는 흑진미(Oryza sativa cv. Heugjinmi) 추출물을 이용하여 항산화 및 미백 관련 기능성 화장품 소재로서의 활용 가능성을 검증하고자 항산화 및 미백 관련 인자들의 효능을 분석하였다. 그 결과, SOD 유사 활성, FRAP, 환원력, xanthine oxidase 저해 활성, elastase저해 활성에서 대조군과 유사한 활성을 나타내었다. Tyrosinase 저해 활성은 열수 추출물에서는 효과가 없었고 에탄올 추출물에서는 59%의 억제 활성을 나타내었다. 에탄올 추출물은 B16F10 세포주에서 생존율에 영향을 미치지 않을 농도인 25, 50, 100 ㎍/mL 농도에서 cellular tyrosinase와 멜라닌 생합성을 저해하는 것으로 확인되었다. 또한, tyrosinase, TRP-1, TRP-2의 mRNA 발현을 확인한 결과는 최고농도인 100 ㎍/mL에서 각각 37%, 51%, 34%의 억제율을 보였다. 따라서 흑진미(O. sativa) 추출물은 항산화 및 미백 관련 기능성 화장품 소재로서의 활용 가능성이 있을 것으로 판단된다.

Abstract ▼ AI-Helper

In this study, the efficacy of antioxidant and whitening factors was analyzed in order to verify the possibility of use as functional cosmetic materials related to antioxidant and whitening by using the extract of Oryza sativa cv. Heugjinmi. As a result, S OD-like activity, FRAP, reducing power, xanthine oxidase inhibitory activity, and elastase inhibitory activity were similar to those of the control group. Tyrosinase inhibitory activity had no effect in hydrothermal extract and 59% inhibitory activity in ethanol extract. Ethanol extract was found to inhibit cellular tyrosinase inhibitory activity and melanin biosynthesis at concentrations of 25, 50, and 100 ㎍/mL, which will not affect survival in the B16F10 cell line. In addition, the results of confirming the mRNA expression of tyrosinase, TRP-1, and TRP-2 showed inhibition rates of 37%, 51%, and 34%, respectively, at the highest concentration of 100 ㎍/mL. Therefore, it is believed that O. sativa extract has potential to be utilized as a functional cosmetic material related to antioxidant and whitening.

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표/그림 (10)

그림 Figure 1. The procedure for extraction from Oryza sativa cv. Heugjinmi (O. sativa).
그림 Figure 2. SOD-like activity assay of O. sativa e xtracts. OS W : O. sativa water extract, OSE : O. sativa 70% ethanol extract, Aa : ascorbic acid. The different letters indicate statistical difference at the p < 0.05 by Duncan's multiple range test. Result are expressed as means ± SD of triplicate data.
그림 Figure 3. FRAP reducing antioxidant power asssay of O. sativa extracts. OSW : O. sativa water extract, OSE : O. sativa 70% ethanol extract, BHA : butylated hydroxy anisole. The different letters indicate statistical difference at the p < 0.05 by Duncan's multiple range test. Result are expressed as means ± SD of triplicate data.
그림 Figure 4. Reducing power assay of O. sativa extracts. OSW : O. sativa water extract, OSE : O. sativa 70% ethanol extract, Aa : ascorbic acid. The different letters indicate statistical difference at the p < 0.05 by Duncan's multiple range test. Result are expressed as means ± SD of triplicate data.
그림 Figure 5. Xanthine oxidase inhibition assay of O. sativa e xtracts. O S W : O. sativa water extract, OSE : O. sativa 70% ethanol extract, BHA : butylated hydroxy anisole. The different letters indicate statistical difference at the p < 0.05 by Duncan's multiple range test. Result are expressed as means ± SD of triplicate data.
그림 Figure 6. Elastase inhibition activity of O. sativa extracts. O. sativa water extract, OSE : O. sativa 70% ethanol extract, Oa : oleanolic acid. The different letters indicate statistical difference at the p < 0.05 by Duncan's multiple range test. Result are expressed as means ± SD of triplicate data.
그림 Figure 7. Tyrosinase inhibition of O. sativa extracts. OSW : O. sativa water extract, OSE : O. sativa 70% ethanol extract, Aa : ascorbic acid. The different letters indicate statistical difference at the p < 0.05 by Duncan's multiple range test. Result are expressed as means ± SD of triplicate data.
그림 Figure 8. Cytotoxicity of O. sativa ethanol e xtract (OS E) o n B16F10 c ells. Cont : B 16F10 cells were n ot t reated O S E. B 16F10 cells were preincubated for 24 h and then treated with variable concentrations of OSE. Result are expressed as means ± SD of triplicate data.
그림 Figure 9. (A) Cellular tyrosinase inhbition of O. sativa ethanol extract (OSE) on B16F10 cells. (B) Melanin production of O. sativa ethanol extract on B16F10 cells. Arbutin 100 : positive control, 100 ug/mL. Nor : B16F10 cells were treated a-MSH and OSE. Cont : B16F10 cells were only treated a-MSH (200 nM) B16F10 cells were preincubated for 24 h and the cells were stimulated α-MSH (200 nM) in the presence of OSE for 48 h. Result are expressed as means ± S D of triplicate data.
그림 Figure 10. RT-PCR of O. sativa ethanol extract (OSE). TRP-1 : tyrosinase related protein 1, TRP-2 : tyrosinase related protein 2, Cont : B16F10 cells w ere not treated a-MS H and OSE. B 16F10 cells were treated w ith α -MS H (200 n M) a nd OSE. R esult a re representative data. Result are expressed as means ± SD of triplicate data.

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