[논문]울릉국화 엑소좀의 항노화 활성 효과 연구

김민하; 윤은정; 김정수; 배소현; 최나영; 박시준; 이현상

doi:10.15230/scsk.2024.50.3.289

[국내논문] 울릉국화 엑소좀의 항노화 활성 효과 연구
Study on the Anti-Aging Activity of Chrysanthemum lucidum Exosomes

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.50 no.3, 2024년, pp.289 - 299

김민하 ((주)에이바이오머티리얼즈) , 윤은정 ((주)에이바이오머티리얼즈) , 김정수 ((주)에이바이오머티리얼즈) , 배소현 ((주)에이바이오머티리얼즈) , 최나영 ((주)에이바이오머티리얼즈) , 박시준 ((주)에이바이오머티리얼즈) , 이현상 ((주)에이바이오머티리얼즈)

초록
AI-Helper

울릉국화(Chrysanthemum lucidum, C. lucidum)는 국화과의 여러해살이풀로, 경북 울릉도에만 분포하는 고유종으로, 이전 연구에서 울릉국화 추출물의 높은 폴리페놀 및 플라보노이드 물질 함량이 높아 우수한 항산화 활성이 보고되어 있으나, 울릉국화 추출물의 주름개선, 세포재생 등과 같은 항노화와 관련된 효능은 잘 알려져 있지 않으며, 울릉국화 유래 엑소좀(C. lucidum-derived extracellular vesicles, ClDEVs)에 대한 활성 연구는 전무한 실정이다. 따라서, 본 연구에서는 in vitro 및 인체적용시험을 통해 ClDEVs에 대한 항노화 활성 효과를 검증하였다. In vitro 세포 실험에서, ClDEVs는 세포 재생을 촉진하고, 콜라겐 합성 유전자인 COL1A1의 발현을 증가시켰고, 피부 장벽 개선 관련 바이오마커 유전자인 LOR 및 FLG의 발현을 증가시켰다. 또한, 노화를 유도시킨 세포에서 노화 관련 바이오마커인 CDKN2A (encodes p16) 및 TP53 (encodes p53) 유전자의 발현을 억제시켰다. 인체적용시험에서 ClDEVs가 함유된 화장료를 4 주간 사용 후 눈가주름 및 팔자주름 개선을 평가한 결과, 4 주 후 통계적으로 유의한 수준의 주름 개선 효과를 나타내었다. 결론적으로 ClDEVs는 주름 개선 및 항노화를 위한 바이오 화장품으로서 높은 응용 가능성을 입증하였다.

Abstract ▼ AI-Helper

Chrysanthemum lucidum (C. lucidum), a perennial herb in the Asteraceae family, is an endemic species found only on Ulleung island in Gyeongsangbuk-do, South Korea. Previous studies have reported that the extract of C. lucidum exhibits excellent antioxidant activity due to its high polyphenol and flavonoid content. However, the anti-aging effects of C. lucidum extract, such as wrinkle improvement and cell regeneration, are not well known, and there has been no research on the activity of C. lucidum-derived extracellular vesicles (ClDEVs). Therefore, this study aimed to verify the anti-aging effects of ClDEVs through in vitro and clinical analyses. In cell experiments, ClDEVs promoted cell regeneration, increased the expression of COL1A1, a gene involved in collagen synthesis, and enhanced the expression of FLG and LOR, a biomarker related to skin barrier improvement. Additionally, ClDEVs suppressed the expression of aging-related biomarkers, such as the CDKN2A (encodes p16) and TP53 (encodes p53) genes, in cells induced to age. In a human clinical trial, after using a cosmetic product containing ClDEVs for 4 weeks, significant improvement in wrinkles around the eyes and nasolabial folds was observed. In conclusion, ClDEVs have demonstrated high potential as a bio-cosmetic ingredient for wrinkle improvement and anti-aging.

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표/그림 (8)

표 Table 1. Pimer Sequence in This Study
그림 Figure 2. HaCaT cell viability as a function of ClDEVs treatment concentration (MTT assay). Results are presented as mean ± SD.
그림 Figure 1. Size and distribution profiles of C. lucidum derived exosomes (ClDEVs) as determined by nanoparticle tracking analysis (NTA), cryo-TEM and Oil red O staining. (A) Size and distribution of ClDEVs using NTA. (B) cryo-TEM analysis. (C) Oil red O staining analysis.
그림 Figure 3. Results from wound healing assays. (A) Percentage of wound closure for 18 h. (B) Cell scratch assay results. Results are presented as mean ± SD. **p < 0.01, ***p < 0.001.
그림 Figure 4. Effect of ClDEVson the expression of collagen biosynthesis related biomarkers. Gene expression of COL1A1 (A) and MMP-1 (B) i n HDF cells. Results a re presented as mean ± SD. **p < 0.01, ***p < 0.001.
그림 Figure 5. Effect of ClDEVson the expression of skin barrier related biomarkers. Gene expression of FLG (A) and LOR (B) in HaCaT cells. Results are presented as mean ± SD. *p < 0 . 05, **p < 0. 01, ***p < 0.001.
그림 Figure 6. Inhibitory effects of ClDEVs on H₂O₂-induced CDKN2A and TP53 in HDF cells. Gene expression of CDKN2A (A) and TP53 (B). Results are presented as mean ± SD. *p < 0 . 05
그림 Figure 7. Results of human application tests for wrinkle improvement on cosmetics containing ClDEVs. Evaluation of improvement in wrinkles around the eyes (A) and in nasolabial folds (B). Results are presented as mean ± SD. ***p < 0.001 (significant difference compared to baseline), ^††p < 0.01 (significant difference compared to the groups).

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