Bilitewski, U.
(Department of Enzyme Technology, Gesellschaft fur Biotechnologische Forschung (GBF), ,, Braunschweig, Germany)
,
Drewes, W.
,
Neermann, J.
,
Schrader, J.
,
Surkow, R.
,
Schmid, R.D.
,
Bradley, J.
To achieve effective bioprocess monitoring, sensing systems are required which are suitable for an on-line determination of substrates, inhibitors, nutrients or products. Such devices may utilise biochemical principles, i.e. the specific interaction of biochemical receptors with their surroundings. ...
To achieve effective bioprocess monitoring, sensing systems are required which are suitable for an on-line determination of substrates, inhibitors, nutrients or products. Such devices may utilise biochemical principles, i.e. the specific interaction of biochemical receptors with their surroundings. They can be constructed either as in situ sensors or as flow-through sensors connected to the process via sampling devices. Hence, characteristic features of an in situ glucose electrode are described, e.g. analytical range, sensitivity and stability. The sensor was based on mediated electron transfer from the enzyme glucose oxidase to the graphite electrode, the mediators being tetrathiafulvalene (TTF) or dimethylferrocene (DMF). Additionally, various flow injection analysis (FIA) systems based on oxidases, which were immobilised either on controlled pore glass or in a membrane, were characterised with respect to analytical ranges and sensitivities and applied to glucose, lactate and glutamate determinations in off-line samples taken from an animal cell cultivation.
To achieve effective bioprocess monitoring, sensing systems are required which are suitable for an on-line determination of substrates, inhibitors, nutrients or products. Such devices may utilise biochemical principles, i.e. the specific interaction of biochemical receptors with their surroundings. They can be constructed either as in situ sensors or as flow-through sensors connected to the process via sampling devices. Hence, characteristic features of an in situ glucose electrode are described, e.g. analytical range, sensitivity and stability. The sensor was based on mediated electron transfer from the enzyme glucose oxidase to the graphite electrode, the mediators being tetrathiafulvalene (TTF) or dimethylferrocene (DMF). Additionally, various flow injection analysis (FIA) systems based on oxidases, which were immobilised either on controlled pore glass or in a membrane, were characterised with respect to analytical ranges and sensitivities and applied to glucose, lactate and glutamate determinations in off-line samples taken from an animal cell cultivation.
Bilitewski 1991 9. Dechema Jahrestagung der Biotechnologen Elektroden zur Simultanbestimmung von Alkohol und Glucose in der Lebensmittel- und Prozesskontrolle
Analyst Bradley 114 375 1989 10.1039/an9891400375 Rapid determination of the glucose content of molasses using a biosensor
Biosensors & Bioelectronics Bradley 6 669 1991 10.1016/0956-5663(91)87020-C Optimisation of a biosensor for in situ fermentation monitoring of glucose concentration
Process Control and Quality Bradley 1 157 1991 Biochemistry based analysis systems for bioprocess monitoring and control
Biosensors & Bioelectronics Dremel 7 133 1992 10.1016/0956-5663(92)90018-I On-line determination of glucose and lactate concentration in animal cell culture based on fibre optic detection of oxygen in flow injection analysis
J. Biotechnol. Filippini 18 153 1991 10.1016/0168-1656(91)90243-O On-line determination of glucose in biotechnological processes: comparison between FIA and an in situ enzyme electrode
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