[논문]Evaluation of hepatitis C virus envelope proteins expressed in E. coli and insect cells for use as tools for antibody screening

Hussy, P.; Faust, H.; Wagner, J.-C.; Schmid, G.; Mous, J.; Jacobsen, H.

doi:10.1016/S0168-8278(97)80450-3

[해외논문] Evaluation of hepatitis C virus envelope proteins expressed in E. coli and insect cells for use as tools for antibody screening

Journal of hepatology : the journal of the European Association for the Study of the Liver, v.26 no.6, 1997년, pp.1179 - 1186

Hussy, P. (aPRP) , Faust, H. , Wagner, J.-C. , Schmid, G. , Mous, J. , Jacobsen, H.

Abstract ▼ AI-Helper

Background/Methods: The two envelope proteins of hepatitis C virus, E1 and E2, were expressed in E. coli and, as secretory proteins, in Sf9 insect cells using recombinant baculoviruses. Co-infection of insect cells with E1 and E2-recombinant baculoviruses was performed, which has been shown to result in formation of E1-E2 dimers. All envelope proteins were purified by Ni2+-NTA chromatography and used for screening of serum samples in a HCV EIA assay. Serum samples of normal blood donors, chronically HCV-infected patients, a mixed titer panel and several sero-conversion panels were screened and compared to test results with Cobas Core Anti-HCV EIA.Results: Screening of the sera of chronically HCV-infected patients (100% positive in Cobas Core Anti-HCV EIA) revealed 10-40% anti-E1 positive sera using different Sf9-expressed, glycosylated proteins and 93% using E. coli-expressed, non-glycosylated E1 protein. When the same sera were tested with different E2 proteins expressed in Sf9 cells and in E. coli, about 70-73% showed anti-E2 reactivity. When the proteins from Sf9 cells co-infected with E1- and E2-recombinant baculoviruses were tested, 70-80% of the same sera showed anti-envelope reactivity.Conclusions: Testing of these patient antisera, and those from the well-characterized mixed titer panel BBI-PHV203, showed that recombinant E1 expressed in E. coli and co-expressed E1 and E2 proteins from Sf9 cells could be used as additional tools for anti-HCV antibody screening.

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DOI : 10.1016/S0168-8278(97)80450-3
European Association for the Study of the Liver : 저널
Elsevier : 저널

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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