[논문]NHE-3 isoform of the NA+/H+ exchanger in human gallbladder - Localization of specific mRNA by in situ hybridization

Silviani, V.; Gastaldi, M.; Planells, R.; Marteau, C.; Massacrier, A.; Cohen, P.; Cau, P.; Gerolami, A.

doi:10.1016/S0168-8278(97)80463-1

[해외논문] NHE-3 isoform of the NA+/H+ exchanger in human gallbladder - Localization of specific mRNA by in situ hybridization

Journal of hepatology : the journal of the European Association for the Study of the Liver, v.26 no.6, 1997년, pp.1281 - 1286

Silviani, V. (aINSERM U.260 ,, Marseilles, France) , Gastaldi, M. , Planells, R. , Marteau, C. , Massacrier, A. , Cohen, P. , Cau, P. , Gerolami, A.

Abstract ▼ AI-Helper

Background/Aims: Electroneutral absorption of NaCl by the gallbladder mucosa is likely to depend at least in part of a Na+/H+ exchanger. In intestine and colon, absorption due to Na+/H+ exchanger is explained by the presence of specific isoforms of the exchanger, the NHE-3 isoform and possibly the NHE-2 isoform. The aim of the present work was to determine whether the mRNAs coding for NHE-2 and NHE-3 are expressed in epithelial cells of human gallbladder.Methods: Total RNAs from human gallbladder were subjected to reverse transcription-polymerase chain reaction using specific primers. No message was observed with NHE-2 specific primers, showing that NHE-2 isoform plays no role in gallbladder absorption. With NHE-3 specific primers, a 239 bp cDNA fragment was obtained and showed a high homology with the NHE-3 isoform, confirming the presence of NHE-3 in the gallbladder wall. This fragment was cloned in a pLitmus vector in order to produce cRNA probes by in vitro transcription. Cellular localization of the NHE-3 mRNA was studied on cryostat sections using the cRNA probes labeled with Digoxigenin-11-UTP, controls included assays with sense probe, antibodies without probe and RNase A treated tissue. A specific staining of the NHE-3 mRNAs was found to be strictly localized to the gallbladder epithelial cells.Results/Conclusions: Expression of NHE-3 in the gallbladder was found only in the absorptive epithelial cells. The NHE-3 isoform of the Na+/H+ exchanger is likely to be involved in water and electrolyte absorption from bile.

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DOI : 10.1016/S0168-8278(97)80463-1
European Association for the Study of the Liver : 저널
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