[논문]In vitro reactivity of cryoglobulin IgM and IgG in hepatitis C virus-associated mixed cryoglobulinemia

Schott, P.; Polzien, F.; Muller-Issberner, A.; Ramadori, G.; Hartmann, H.

doi:10.1016/S0168-8278(98)80197-9

[해외논문] In vitro reactivity of cryoglobulin IgM and IgG in hepatitis C virus-associated mixed cryoglobulinemia

Journal of hepatology : the journal of the European Association for the Study of the Liver, v.28 no.1, 1998년, pp.17 - 26

Schott, P. (Department of Medicine, Division of Gastroenterology and Endocrinology, Georg-August-University, ,, Gottingen, Germany) , Polzien, F. , Muller-Issberner, A. , Ramadori, G. , Hartmann, H.

Abstract ▼ AI-Helper

Background/Aims: Mixed cryoglobulinemia is frequently associated with chronic hepatitis C virus infection. We aimed to clarify the mechanism, kinetics and participating proteins in cryoprecipitate formation, whcih are still being debated.Methods: Eighteen patients with cryoglobulinemia were studied. Isolated serum cryoprecipitates and purified cryoglobulin IgM and IgG fractions were analyzed in vitro by turbidimetry for temperature-dependent complex formation. Immunoglobulin reactivity, i.e., in cryoprecipitates and in cryoglobulin-free sera, was studied using immunoblot and enzyme immunoassays. HCV RNA was detected by reverse transcriptase/polymerase chain reaction.Results: By turbidimetry, purified cryo-IgM precipitated (in the absence of HCV RNA) with cryo-IgG as well as with non-cryoglobulin IgG and with IgG Fc or F(ab')2 fragments. In contrast, purified cryo-IgG did not precipitate with non-cryoglobulin IgM. Anti-HCV IgG reactivity was found in cryoglobulin-free sera, in cryoprecipitates and in purified cryoglobulin IgG fractions. The respective titers were similar. Purified cryo-IgM did not react to HCV-encoded proteins. Binding of cryo-IgM to heterologous IgG was inhibited by intact IgG (up to a mean of about 52%) as well as by IgG Fc (33%) and F(ab')2 fragments (17%). Binding of cryo-IgM to IgG was enhanced at low temperature (4oC vs. 37oC), particularly for type III cryoglobulin IgM.Conclusions: In hepatitis C virus-associated cryoglobulinemia the vitro precipitate formation depended on cryo-IgM, while IgG appeared to act as an unspecific antigenic partner. Hepatitis C viral particles were probably not required. Cryo-IgM binding occurred primarily to intact IgG. Anti-HCV reactivity of either cryo-IgM or cryo-IgG was not necessary for precipitate formation. Regarding the pathogenesis, a direct hepatitis C virus protein-dependent stimulation of B-cells producing cryo-IgM seems to be unlikely.

참고문헌 (45)

Bull Johns Hopkins Hosp Wintrobe 52 156 1933 Hyperproteinemia associated with multiple myeloma
Am J Med Sci Lerner 214 410 1947 10.1097/00000441-194710000-00009 Studies of cryoglobulins. I. Unusual purpura associated with the presence of a high concentration of cryoglobulin (cold precipitable serum globulin)

상세보기
Am J Med Meltzer 40 828 1966 10.1016/0002-9343(66)90199-9 Cryoglobulinemia: a study of 29 patients. I. IgG and IgM cryoglobulins and factors effecting cryoprecipitability

상세보기
Am J Med Gorevic 69 287 1980 10.1016/0002-9343(80)90390-3 Mixed cryoglobulinemia: clinical aspects and long-term follow-up of 40 patients

상세보기
Am J Med Brouet 57 775 1974 10.1016/0002-9343(74)90852-3 Biologic and clinical significance of cryoglobulins. A report of 86 cases

상세보기
Infection Ferri 19 417 1991 10.1007/BF01726453 Antibodies against hepatitis C virus in mixed cryoglobulinemia patients

상세보기
Q J Med Monti 88 115 1995 Cryoglobulinaemias: a multi-centre study of the early clinical and laboratory manifestations of primary and secondary disease
Clin Exp Immunol Pechere-Bertschi 89 419 1992 10.1111/j.1365-2249.1992.tb06973.x Hepatitis C: a possible etiology for cryoglobulinaemia type II

상세보기
Eur J Clin Invest Ferri 23 399 1993 10.1111/j.1365-2362.1993.tb00782.x Hepatitis C virus and mixed cryoglobulinaemia

상세보기
Arthr Rheum Abel 36 1341 1993 10.1002/art.1780361003 Hepatitis C virus infection in type II mixed cryoglobulinemia

상세보기
Clin Exp Immunol Wong 104 25 1996 10.1046/j.1365-2249.1996.d01-639.x Incidence, character and clinical relevance of mixed cryoglobulinaemia in patients with chronic hepatitis C virus infection

상세보기
Z Gastroenterol Hartmann 33 643 1995 Cryoglobulinemia in chronic hepatitis C virus infection: prevalence, clinical manifestations, response to interferon treatment and analysis of cryoprecipitates

상세보기
Gastroenterology Lunel 106 1291 1994 10.1016/0016-5085(94)90022-1 Cryoglobulinemia in chronic in diseases: role of hepatitis C virus and liver damage

상세보기
Ital J Gastroenterol Adinolfi 28 1 1996 Epidemiology, clinical spectrum and prognostic value of mixed cryoglobulinemia in hepatitis C virus patients: a prospective study
Scand J Immunol Agnello 42 179 1995 10.1111/j.1365-3083.1995.tb03643.x The aetiology of mixed cryoglobulinaemia associated with hepatitis C virus infection

상세보기
J Hepatol Frangeul 25 427 1996 10.1016/S0168-8278(96)80200-5 Hepatitis C virus genotypes and subtypes in patients with hepatitis C, with and without cryoglobulinemia

상세보기
Q J Med Sinico 88 805 1995 Hepatitis C virus genotype in patients with essential mixed cryoglobulinemia
Ann Intern Med Zignego 124 31 1996 10.7326/0003-4819-124-1_Part_1-199601010-00006 Hepatitis C virus genotype analysis in patients with type II mixed cryoglobulinemia

상세보기
Clin Exp Immunol Gabrielli 105 59 1996 10.1046/j.1365-2249.1996.d01-720.x Differential humoral immune response against hepatitis C virus antigenic synthetic peptides in infected patients with and without mixed cryoglobulinemia

상세보기
Ann Intern Med Misiani 117 573 1992 10.7326/0003-4819-117-7-573 Hepatitis C virus infection in patients with essential mixed cryoglobulinemia

상세보기
Clin Exp Immunol L'Abbate 94 313 1993 10.1111/j.1365-2249.1993.tb03449.x IgM and IgG antibodies to hepatitis C virus in patients with mixed cryoglobulinemia

상세보기
Hepatology Aiyama 24 1346 1996 10.1002/hep.510240605 Hypervariable region sequence in cryoglobulin-associated hepatitis C virus in sera of patients with chronic hepatitis C. Relationship to antibody response against hypervariable region genome

상세보기
J Immunol Geltner 125 1530 1980 10.4049/jimmunol.125.4.1530 Antiidiotypic activity in the IgM fractions of mixed cryoglobulin

상세보기
Springer Semin Immunopathol Chen 10 35 1988 10.1007/BF02054022 Cross-reacting idiotypes on cryoprecipitating rheumatoid factor

상세보기
J Biol Chem Chavin 244 1345 1969 10.1016/S0021-9258(18)91849-1 Studies on antigen-binding activity of macroglobulin antibody subunits and their enzymatic fragments

상세보기
Dtsch Med Wochenschr Schubothe 35 1786 1970 10.1055/s-0028-1108729

상세보기
Nature Laemli 227 680 1970 10.1038/227680a0 Cleavage of structural proteins during assembly of the head of bacteriophage T4

상세보기
Proc Natl Acad Sci Towbin 76 4350 1979 10.1073/pnas.76.9.4350 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

상세보기
Immunochemistry Middaugh 15 171 1978 10.1016/0161-5890(78)90146-3 Molecular basis for the temperature-dependent insolubility of cryoglobulins. IV. Structural studies of the IgM monoclonal cryoglobulin McE

상세보기
J Mol Biol Gaskin 89 737 1974 10.1016/0022-2836(74)90048-5 Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules

상세보기
Gastroenterology Farivar 71 490 1976 10.1016/S0016-5085(76)80461-1 Cryoprotein complexes and peripheral neuropathy in a patient with chronic active hepatitis

상세보기
Lancet Feizi ii 873 1969 10.1016/S0140-6736(69)92329-0 Immune-complex disease of the kidney associated with chronic hepatitis and cryoglobulinaemia

상세보기
Clin Exp Immunol Florin-Christensen 16 599 1974 Cryoglobulins in acute and chronic liver diseases

상세보기
Gut Jori 18 245 1977 10.1136/gut.18.3.245 Incidence and immunochemical features of serum cryoglobulin in chronic liver disease

상세보기
A role for chronic hepatitis C virus infection in a patient with cutaneous vasculitis, cryoglobulinemia, and chronic liver disease Schirren 40 1221 1995 Effective therapy with interferon-α
Med Microbiol Immunol Thomssen 182 329 1993 10.1007/BF00191948 Density heterogeneities of hepatitis C virus in human sera due to the binding of β-lipoproteins and immunoglobulins

상세보기
J Gen Virol Müller 74 669 1993 10.1099/0022-1317-74-4-669 Peripheral blood leukocytes serve as a possible extrahepatic site for hepatitis C virus replication

상세보기
J Hepatol Zignego 15 382 1992 10.1016/0168-8278(92)90073-X Infection of peripheral mononuclear blood cells by hepatitis C virus

상세보기
Blood Ferri 82 3701 1993 10.1182/blood.V82.12.3701.3701 Infection of peripheral blood mononuclear cells of hepatitis C virus in mixed cryoglobulinemia

상세보기
Blood Pozzato 84 3047 1994 10.1182/blood.V84.9.3047.3047 Low-grade malignant lymphoma, hepatitis C virus infection, and mixed cryoglobulinemia

상세보기
Br J Haematol Ferri 88 392 1994 10.1111/j.1365-2141.1994.tb05036.x Hepatitis C virus infection in patients with non-Hodgkin's lymphoma

상세보기
Blood Ferri 81 1132 1993 10.1182/blood.V81.5.1132.1132 Interferon-α in mixed cryoglobulinemia patients: a randomized, crossover-controlled trial

상세보기
Cancer Mazzaro 77 2604 1996 10.1002/(SICI)1097-0142(19960615)77:12<2604::AID-CNCR26>3.0.CO;2-V Regression of monoclonal B-cell expansion in patients affected by mixed cryoglobulinemia responsive to α-interferon therapy

상세보기
J Hepatol Polzien 27 63 1997 10.1016/S0168-8278(97)80281-4 Interferon-α treatment of hepatitis C virus-associated mixed cryoglobulinemia

상세보기
J Immunol Peters 137 3153 1986 10.4049/jimmunol.137.10.3153 Effect of interferon-α on immunoglobulin synthesis by human B cells

상세보기

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

원문 URL 링크

DOI : 10.1016/S0168-8278(98)80197-9
European Association for the Study of the Liver : 저널
Elsevier : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

저작권 관리 안내

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증