[논문]Density analysis of hepatitis C virus particle population in the circulation of infected hosts: implications for virus neutralization or persistence

Kanto, T.; Norio, H.; Takehara, T.; Hideki, H.; Eiji, M.; Masafumi, N.; Akinori, K.; Hideyuki, F.; Takenobu, K.

doi:10.1016/0168-8278(95)80107-3

[해외논문] Density analysis of hepatitis C virus particle population in the circulation of infected hosts: implications for virus neutralization or persistence

Journal of hepatology : the journal of the European Association for the Study of the Liver, v.22 no.4, 1995년, pp.440 - 448

Kanto, T. (First Department of Medicine, Osaka University Medical School, Suita, ,, Osaka, Japan) , Norio, H. , Takehara, T. , Hideki, H. , Eiji, M. , Masafumi, N. , Akinori, K. , Hideyuki, F. , Takenobu, K.

Abstract ▼ AI-Helper

Hepatitis C virus has a low buoyant density in sucrose, but high-density particles are often observed in hepatitis C virus infection.To investigate the characteristics of circulating hepatitis C virus particles and their association with liver disease progression, we examined sera from two histologically normal hepatitis C virus carriers, 20 chronic hepatitis patients and five acute hepatitis C patients. The supernatants obtained after immunoprecipitation with anti-immunoglobulins antibody were subjected to sucrose equilibrium centrifugation. HCV-RNA positive fractions separated after the treatments were further examined for immunoprecipitation with anticore hepatitis C virus antibody. We separated hepatitis C virus particle populations according to the density difference on 35% sucrose with centrifugation. The proportions of high and low density particles in hepatitis C virus population were determined by means of competitive reverse transcription and polymerase chain reaction.Circulating hepatitis C virus particles in chronically infected patients could be separated into two populations: those immunoglobulin-bound with high densities and -unbound with low densities. Patients with severe liver inflammation had high-density hepatitis C virus that did not precipitate with anti-immunoglobulins but with anti-core hepatitis C virus antibodies. Thus, hepatitis C virus particle populations consist of low-density virions and high-density immune complexes and/or nucleocapsids. Among the chronic hepatitis patients, the dominant population shifted from low-density to high-density particles with the progression of liver disease. In acute hepatitis patients, this density shift was observed with alanine aminotransferase normalizations.Therefore, the major hepatitis C virus populations change from virion to immune complex and/or nucleocapsid with the progression of liver disease or inflammation.

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DOI : 10.1016/0168-8278(95)80107-3
European Association for the Study of the Liver : 저널
Elsevier : 저널

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