[논문]The effect of anti-CD3-immunotoxin on T lymphocyte function in vitro

doi:10.1016/S0966-3274(98)80035-X

[해외논문] The effect of anti-CD3-immunotoxin on T lymphocyte function in vitro

Transplant immunology, v.6 no.1, 1998년, pp.53 - 59

Meng, Gang (University of Alabama at Birmingham, Birmingham, AlabamaU.S.A.) , Jiang, Yi (University of Alabama at Birmingham, Birmingham, AlabamaU.S.A.) , Hubbard, William (University of Alabama at Birmingham, Birmingham, AlabamaU.S.A.) , Thomas, Judith M (Address for correspondence: JM Thomas, Division of Immunology, Department of Surgery, Transplant Center BDB #802, 1808 7th Avenue South, Birmingham, AL, 35294, USA)

Abstract ▼ AI-Helper

AbstractRecent advances in the design of immunotoxins (IT) have yielded significant improvements. FN18-CRM9, a construct of anti-Cd3ε mAb FN18 and mutated diphtheria toxin CRM9 has exhibited high specificity, low systemic toxicity and unusual efficacy compared to previous iterations of immunotoxins. Others and we have examined anti-CD3-IT for the purpose of inducing immunological tolerance through selective ablation of T cells in rhesus macaques and have obtained encouraging results. In order to characterize its mode of action, we have examined its effects on peripheral blood and lymph node T cell killing in vitro. We have studied the cytotoxic mechanism induced by this anti-CD3-IT as well as its effects on proliferation, phenotypic changes and cytokine production (IL2, IFNγ and TNFα). The results indicate that anti-CD3-IT was highly specific for T cell killing at doses as low as 1 × 106 μg/ml and showed a maximal effect at 48 h after exposure. The toxicity was restricted to T cells, as B cells and other bystander cells were spared. This immunotoxin was shown to induce T cell apoptosis, as assessed by TUNEL assay, DNA content and cytotoxicity. Fas expression was upregulated on T cells within 24 h after in vitro exposure to anti-CD3-IT, suggesting an early T cell activation phase prior to T cell death. T cell killing was manifest as an early cell cycle arrest at the G1/S phase transition, which appeared to virtually eliminate the production of cytokines. These finding corroborate the temporal, specificity and quantitative patterns for anti-CD3 immunotoxin administration previously observed in vivo.

참고문헌 (29)

Minerva Chirurgica Thomas 46 51 1991 Strategies for developing organ transplant tolerance
Clin Transplantation Lafferty 8 181 1994 10.1111/j.1399-0012.1994.tb00093.x A contemporary view of transplant tolerance: an immunologist's perspective

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Thomas 143-158 1995 A preclinical view of transplant tolerance and chimerism: tolerance induction by postransplant lymphoid irradiation and donor bone marrow
J Heart Lung Transplantation Nikolic 15 1171 1996 Clonal detection as a mechanism of transplantation tolerance [Review]
Thomas 59-79 1996 Tolerance induction: basis concepts and therapeutic approaches
Transplantation Knechtle 63 1 1997 10.1097/00007890-199701150-00002 FN18-CRM9 immunotoxin promotes tolerance in primate renal allografts

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Bone Marrow Transplantation Sharabi 9 191 1992 Mixed allogeneic chimeras prepared by a non-mycloablative regimen: requirement for chimerism to maintain tolerance
Transplantation Moses 48 774 1989 10.1097/00007890-198911000-00010 Cardiac allograft survival across major histocompatibility complex barriers in the rhesus monkey following T lymphocyte-depleted autologous marrow transplantation

상세보기
Transplantation Myburgh 29 405 1980 10.1097/00007890-198005000-00012 Transplantation tolerance in primates following total lymphoid irradiation and allogenic bone marrow injection. II. Renal Allografts

상세보기
Transplantation Thomas 47 209 1989 10.1097/00007890-198902000-00002 Promotion of incompatible allograft acceptance in rhesus monkeys given posttransplant an tithymocyte globulin and donor bone marrow. II. Effects of adjuvant immunosuppressive drugs

상세보기
Am Soc Transplant Surg Kawai 1 31 1994 Multilineage mixed chimerism and transplantation tolerance in cynomolgus monkeys [Abstract]
Transplantation Science Thomas 3 69 1989 Transplantation tolerance in nonhuman primates: a case for vetocells
Transplant Proc Carver 23 480 1991 Post-transplant TLI is effective in nonhuman primates if combined with rabbit ATG

상세보기
Transplantation Thomas 51 198 1991 10.1097/00007890-199101000-00032 Kidney allograph tolerance in primates without chronic immunosuppression: The role of veto cells

상세보기
Transplantation Thomas 53 247 1992 10.1097/00007890-199202010-00001 The development of a posttransplant TLI treatment strategy that promotes organ allograft acceptance without chronic immunosuppression

상세보기
Transplantation Thomas 64 124 1997 10.1097/00007890-199707150-00022 A novel anti-CD3 immunotoxin given peritransplant with donor bone marrow induces operational tolerance to kidney allografts

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J Immunol Ildstad 136 28 1986 10.4049/jimmunol.136.1.28 Effect of Selective T cell depletion of host and/or donor bone marrow on lymphopoietic repopulation, tolerance, and graft-vs-host disease in mixed allogenic chimeras (B10 + B10.D2-B10)

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J Immunotherapy Emph Tummor Immunol Neville 19 85 1996 10.1097/00002371-199603000-00001 A new reagent for the induction of T-cell depletion, antiCD3-CRM9

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J Controlled Release Neville 24 133 1995 10.1016/0168-3659(93)90173-3 Anti-T cell immunotoxins. A look at post-endocytic receptor-mediated routing

상세보기
Immunol Today Wawrzynczak 13 381 1992 10.1016/0167-5699(92)90084-K Immunotoxins: the power and the glory

상세보기
Br J Cancer Wu 75 1347 1997 10.1038/bjc.1997.228 Enhancement of immunotoxin activity using chemical and biological reagents [Review]

상세보기
Semin Oncol Chen 23 148 1996 Novel genetic immunotoxins and intracellular antibodies for cancer therapy [Review]
Current Opin Immunol Ghetie 6 707 1994 10.1016/0952-7915(94)90073-6 Recent developments in immunotoxin therapy [Review]

상세보기
Transplantation Fechnet 63 1339 1997 10.1097/00007890-199705150-00023 Split tolerance induced by immunotoxin in a rhesus kidney allograft model

상세보기
Eur J Immunol Nooij 17 1089 1987 10.1002/eji.1830170803 The effect of skin allograft survival of a monoclonal antibody specific for a polymorphic CD3-like cell surface molecule in rhesus monkeys

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Immunology Nooij 59 611 1986 Polymorphism for RhT3 a CD-3 like cell surface antigen, expressed on rhesus monkey T lymphocytes

상세보기
J Immmunol Armitage 150 3671 1993 10.4049/jimmunol.150.9.3671 Human B cell proliferation and Ig secretion induced by recombinant CD40 ligand are modulated by soluble cytokines

상세보기
J Immunol Grabstein 150 3141 1993 10.4049/jimmunol.150.8.3141 The regulation of T cell-dependent antibody formation in vitro by CD40 ligand and IL-2

상세보기
Proc Natl Acad Sci USA Hancock 93 13967 1996 10.1073/pnas.93.24.13967 Costimulatory function and expression of CD40 ligand, CD80 and CD86 in vascularized murine cardiac allograft rejection

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DOI : 10.1016/S0966-3274(98)80035-X
OCLC ECO : 저널
Elsevier : 저널 > 논문

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