[논문]Role of the Nucleoid-Associated Protein H-NS in the Synthesis of Virulence Factors in the Phytopathogenic Bacterium Erwinia chrysanthemi

Nasser, William; Faelen, Michel; Hugouvieux-Cotte-Pattat, Nicole; Reverchon, Sylvie

doi:10.1094/mpmi.2001.14.1.10

[해외논문] Role of the Nucleoid-Associated Protein H-NS in the Synthesis of Virulence Factors in the Phytopathogenic Bacterium Erwinia chrysanthemi 원문보기

Molecular plant-microbe interactions : MPMI, v.14 no.1, 2001년, pp.10 - 20

Nasser, William , Faelen, Michel , Hugouvieux-Cotte-Pattat, Nicole , Reverchon, Sylvie

Abstract ▼ AI-Helper

The ability of the enterobacterium Erwinia chrysanthemi to induce pathogenesis in plant tissue is strongly related to the massive production of plant-cell-wall-degrading enzymes (pectinases, cellulases, and proteases). Additional factors, including flagellar proteins and exopolysaccharides (EPS), also are required for the efficient colonization of plants. Production of these virulence factors, particularly pectate lyases, the main virulence determinant, is tightly regulated by environmental conditions. The possible involvement of the protein H-NS in this process was investigated. The E. chrysanthemi hns gene was cloned by complementation of an Escherichia coli hns mutation. Its nucleotide sequence contains a 405-bp open reading frame that codes for a protein with 85% identity to the E. coli H-NS protein. An E. chrysanthemi hns mutant was constructed by reverse genetics. This mutant displays a reduced growth rate and motility but an increased EPS synthesis and sensitivity toward high osmolarity. Furthermore, pectate lyase production is dramatically reduced in this mutant. The hns mutation acts on at least two conditions affecting pectate lyase synthesis: induction of pectate lyase synthesis at low temperatures (25°C) is no longer observed in the hns mutant and induction of pectate lyase production occurs in the late stationary growth phase in the hns background, instead of in the late exponential growth phase as it does in the parental strain. Moreover, the E. chrysanthemi hns mutant displays reduced virulence on plants. Taken together, these data suggest that H-NS plays a crucial role in the expression of the virulence genes and in the pathogenicity of E. chrysanthemi.

참고문헌 (60)

Atlung, Tove, Ingmer, Hanne. H‐NS: a modulator of environmentally regulated gene expression. Molecular microbiology, vol.24, no.1, 7-17.

상세보기
Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. 1987. Current Protocols in Molecular biology. Wiley Interscience, New York.
Microbiol. Rev. Bachmann B. S. 130 54 1990 10.1128/mr.54.2.130-197.1990

상세보기
FEMS Microbiol. Lett. Bardonnet N. 243 93 1992
BARRAS, F. The ?-glucosides metabolism in Erwinia chrysanthemi: Preliminary analysis and comparison to Escherichia coli systems. FEMS microbiology reviews, vol.5, no.1, 143-147.

상세보기
Barron, A, May, G, Bremer, E, Villarejo, M. Regulation of envelope protein composition during adaptation to osmotic stress in Escherichia coli. Journal of bacteriology, vol.167, no.2, 433-438.

상세보기
Bauer, David W.. Erwinia chrysanthemi hrpGenesand Their Involvement in Soft Rot Pathogenesisand Elicitation of the Hypersensitive Response. Molecular plant-microbe interactions : MPMI, vol.7, no.5, 573-.

상세보기
Bertin, Philippe, Benhabiles, Nora, Krin, Evelyne, Laurent‐Winter, Christine, Tendeng, Christian, Turlin, Evelyne, Thomas, Annick, Danchin, Antoine, Brasseur, Robert. The structural and functional organization of H‐NS‐like proteins is evolutionarily conserved in Gram‐negative bacteria. Molecular microbiology, vol.31, no.1, 319-329.

상세보기
BOURSON, C., FAVEY, S., REVERCHON, S., ROBERT-BAUDOUY, J.. Regulation of the expression of a pelA:: uidA fusion in Erwinia chrysanthemi and demonstration of the synergistic action of plant extract with polygalacturonate on pectate lyase synthesis. The Journal of general microbiology, vol.139, no.1, 1-9.

상세보기
Castillo, A, Reverchon, S. Characterization of the pecT control region from Erwinia chrysanthemi 3937. Journal of bacteriology, vol.179, no.15, 4909-4918.

상세보기
Collmer, A, Keen, N T. The Role of Pectic Enzymes in Plant Pathogenesis. Annual review of phytopathology, vol.24, 383-409.

상세보기
Colonna, B, Casalino, M, Fradiani, P A, Zagaglia, C, Naitza, S, Leoni, L, Prosseda, G, Coppo, A, Ghelardini, P, Nicoletti, M. H-NS regulation of virulence gene expression in enteroinvasive Escherichia coli harboring the virulence plasmid integrated into the host chromosome. Journal of bacteriology, vol.177, no.16, 4703-4712.

상세보기
Condemine, Guy, Castillo, Arnaud, Passeri, Fabrice, Enard, Corine. The PecT Repressor Coregulates Synthesis of Exopolysaccharides and Virulence Factors in Erwinia chrysanthemi. Molecular plant-microbe interactions : MPMI, vol.12, no.1, 45-52.

상세보기
Dattananda, C S, Gowrishankar, J. Osmoregulation in Escherichia coli: complementation analysis and gene-protein relationships in the proU locus. Journal of bacteriology, vol.171, no.4, 1915-1922.

상세보기
Genetics Defez R. 11 97 1981 10.1093/genetics/97.1.11

상세보기
Dorman, Charles J, Hinton, Jay C.D, Free, Andrew. Domain organization and oligomerization among H-NS-like nucleoid-associated proteins in bacteria. Trends in microbiology, vol.7, no.3, 124-128.

상세보기
Hassouni, Mohammed El, Chambost, Jean Pierre, Expert, Dominique, Van Gijsegem, Frederique, Barras, Frederic. The minimal gene set member msrA , encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi. Proceedings of the National Academy of Sciences of the United States of America, vol.96, no.3, 887-892.

상세보기
Enard, C, Diolez, A, Expert, D. Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system. Journal of bacteriology, vol.170, no.6, 2419-2426.

상세보기
Falconi, Maurizio, Higgins, N. Patrick, Spurio, Roberto, Pon, Cynthia L., Gualerzi, Claudio O.. Expression of the gene encoding the major bacterial nucleoid protein H‐NS is subject to transcriptional auto‐repression. Molecular microbiology, vol.10, no.2, 273-282.

상세보기
Fleischmann, RD, Adams, MD, White, O, Clayton, RA, Kirkness, EF, Kerlavage, AR, Bult, CJ, Tomb, JF, Dougherty, BA, Merrick, JM, al., et. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science, vol.269, no.5223, 496-512.

상세보기
Franza, Thierry, Sauvage, Christele, Expert, Dominique. Iron Regulation and Pathogenicity in Erwinia chrysanthemi 3937: Role of the Fur Repressor Protein. Molecular plant-microbe interactions : MPMI, vol.12, no.2, 119-128.

상세보기
Gill Jr, P R, Warren, G J. An iron-antagonized fungistatic agent that is not required for iron assimilation from a fluorescent rhizosphere pseudomonad. Journal of bacteriology, vol.170, no.1, 163-170.

상세보기
10.1002/(SICI)1096-9896(199604)178:4<447::AID-PATH489>3.0.CO;2-N

상세보기
10.1128/9781555818319.ch15 Gottesman, S. 1995. Regulation of capsule synthesis: Modification of a two component paradigm by an accessory unstable protein. Pages 253-262 in: Two-Component Signal Transduction. J. A. Hoch and T. J. Silhavy, eds. American Society for Microbiology, Washington, DC.
Goyard, Sophie, Bertin, Philippe. Characterization of BpH3, an H‐NS‐like protein in Bordetella pertussis. Molecular microbiology, vol.24, no.4, 815-823.

상세보기
Harrison, Julia A., Pickard, Derek, Higgins, Christopher F., Khan, Anjam, Chatfield, Stephen N., Ali, Tahir, Dorman, Charles J., Hormaeche, Carlos E., Dougan, Gordon. Role of hns in the virulence phenotype of pathogenic salmonellae. Molecular microbiology, vol.13, no.1, 133-140.

상세보기
Higgins, C.F., Dorman, C.J., Stirling, D.A., Waddell, L., Booth, I.R., May, G., Bremer, E.. A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli. Cell, vol.52, no.4, 569-584.

상세보기
Hinton, Jay C. D., Santos, Diogenes S., Seirafi, Alexander, Hulton, Christopher S. J., Pavitt, Graham D., Higgins, Christopher F.. Expression and mutational analysis of the nucleoid‐associated protein H‐NS of Salmonella typhimurium. Molecular microbiology, vol.6, no.16, 2327-2337.

상세보기
Hugouvieux-Cotte-Pattat, Nicole, Condemine, Guy, Nasser, William, Reverchon, Sylvie. REGULATION OF PECTINOLYSIS IN ERWINIA CHRYSANTHEMI. Annual review of microbiology, vol.50, 213-257.

상세보기
J. Bacteriol. Hugouvieux-Cotte-Pattat N. 248 162 1985 10.1128/jb.162.1.248-255.1985

상세보기
Hulton, C.S.J., Seirafi, A., Hinton, J.C.D., Sidebotham, J.M., Waddell, L., Pavitt, G.D., Owen-Hughes, T., Spassky, A., Buc, H., Higgins, C.F.. Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria. Cell, vol.63, no.3, 631-642.

상세보기
James, V., Hugouvieux-Cotte-Pattat, N.. Regulatory systems modulating the transcription of the pectinase genes of Erwinia chrysanthemi are conserved in Escherichia coli. Microbiology, vol.142, no.9, 2613-2619.

상세보기
La Teana, A, Brandi, A, Falconi, M, Spurio, R, Pon, C L, Gualerzi, C O. Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS.. Proceedings of the National Academy of Sciences of the United States of America, vol.88, no.23, 10907-10911.

상세보기
Lejeune, P, Danchin, A. Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.. Proceedings of the National Academy of Sciences of the United States of America, vol.87, no.1, 360-363.

상세보기
López-Solanilla, Emilia, García-Olmedo, Francisco, Rodríguez-Palenzuela, Pablo. Inactivation of the sapA to sapF Locus of Erwinia chrysanthemi Reveals Common Features in Plant and Animal Bacterial Pathogenesis. The Plant cell, vol.10, no.6, 917-924.

상세보기
J. Biol. Chem. Lucht J. M. 6578 269 1994 10.1016/S0021-9258(17)37411-2

상세보기
Maurelli, A T, Sansonetti, P J. Identification of a chromosomal gene controlling temperature-regulated expression of Shigella virulence.. Proceedings of the National Academy of Sciences of the United States of America, vol.85, no.8, 2820-2824.

상세보기
Miller, J. H. 1972. Experiment in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, U.S.A.
Moran, Francis, Nasuno, Seiichi, Starr, Mortimer P.. Extracellular and intracellular polygalacturonic acid trans-eliminases of Erwinia carotovora. Archives of biochemistry and biophysics, vol.123, no.2, 298-306.

상세보기
J. Bacteriol. Mukherjee A. 3629 180 1998 10.1128/JB.180.14.3629-3634.1998

상세보기
Mulholland, Vincent, Hinton, Jay C. D., Sidebotham, Julie, Toth, Ian K., Hyman, Lisabeth J., Perombelon, Michel C. M., Reeves, Philip J., Salmond, George P. C.. A pleiotropic reduced virulence (Rvi⁻) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens. Molecular microbiology, vol.9, no.2, 343-356.

상세보기
Nasser, W., Reverchon, S., Condemine, G., Robert-Baudouy, J.. Specific Interactions of Erwinia chrysanthemi KdgR Repressor with Different Operators of Genes Involved in Pectinolysis. Journal of molecular biology, vol.236, no.2, 427-440.

상세보기
Nasser, William, Robert‐Baudouy, Janine, Reverchon, Sylvie. Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes. Molecular microbiology, vol.26, no.5, 1071-1082.

상세보기
Nasser, William, Bouillant, Marie Louise, Salmond, George, Reverchon, Sylvie. Characterization of the Erwinia chrysanthemi expI-expR locus directing the synthesis of two N‐acyl‐homoserine lactone signal molecules. Molecular microbiology, vol.29, no.6, 1391-1405.

상세보기
Nomura, Kinya, Nasser, William, Kawagishi, Hirokazu, Tsuyumu, Shinji. The pir gene of Erwinia chrysanthemi EC16 regulates hyperinduction of pectate lyase virulence genes in response to plant signals. Proceedings of the National Academy of Sciences of the United States of America, vol.95, no.24, 14034-14039.

상세보기
Praillet, Thierry, Nasser, William, Robert‐Baudouy, Janine, Reverchon, Sylvie. Purification and functional characterization of PecS, a regulator of virulence‐factor synthesis in Erwinia chrysanthemi. Molecular microbiology, vol.20, no.2, 391-402.

상세보기
Resibois, A., Colet, M., Faelen, M., Schoonejans, E., Toussaint, A.. &phis;EC2, a new generalized transducing phage of Erwinia chrysanthemi. Virology, vol.137, no.1, 102-112.

상세보기
Reverchon, S, Expert, D, Robert-Baudouy, J, Nasser, W. The cyclic AMP receptor protein is the main activator of pectinolysis genes in Erwinia chrysanthemi. Journal of bacteriology, vol.179, no.11, 3500-3508.

상세보기
Reverchon, S., Nasser, W., Robert‐Baudouy, J.. Characterization of kdgR, a gene of Erwinia chrysanthemi that regulates pectin degradation. Molecular microbiology, vol.5, no.9, 2203-2216.

상세보기
Reverchon, Sylvie, Nasser, William, Robert‐Baudouy, Janine. pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi. Molecular microbiology, vol.11, no.6, 1127-1139.

상세보기
Reverchon, Sylvie, Bouillant, Marie Louise, Salmond, George, Nasser, William. Integration of the quorum‐sensing system in the regulatory networks controlling virulence factor synthesis in Erwinia chrysanthemi. Molecular microbiology, vol.29, no.6, 1407-1418.

상세보기
J. Bacteriol. Roeder D. L. 51 164 1985 10.1128/jb.164.1.51-56.1985

상세보기
Sauvage, Christel. Differential Regulation by Iron ofErwinia chrysanthemiPectate Lyases: Pathogenicity of Iron Transport Regulatory (cbr)Mutants. Molecular plant-microbe interactions : MPMI, vol.7, no.1, 71-.

상세보기
Schoonejans, E, Expert, D, Toussaint, A. Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants. Journal of bacteriology, vol.169, no.9, 4011-4017.

상세보기
Sledjeski, D, Gottesman, S. A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli.. Proceedings of the National Academy of Sciences of the United States of America, vol.92, no.6, 2003-2007.

상세보기
Surgey, N, Robert-Baudouy, J, Condemine, G. The Erwinia chrysanthemi pecT gene regulates pectinase gene expression. Journal of bacteriology, vol.178, no.6, 1593-1599.

상세보기
Appl. Environ. Microbiol. Teather R. M. 777 43 1982 10.1128/aem.43.4.777-780.1982

상세보기
Ueguchi, Chiharu, Kakeda, Minoru, Mizuno, Takeshi. Autoregulatory expression of the Escherichia coli hns gene encoding a nucleoid protein: H-NS functions as a repressor of its own transcription. Molecular & general genetics : MGG, vol.236, no.2, 171-178.

상세보기
Ussery, D.W., Hinton, J.C.D., Jorji, B.J.A.M., Granum, P.E., Seirafi, A., Stephen, R.J., Tupper, A.E., Berridge, G., Sidebotham, J.M., Higgins, C.F.. The chromatin-associated protein H-NS. Biochimie, vol.76, no.10, 968-980.

상세보기
Zhang, A, Belfort, M. Nucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein.. Nucleic acids research, vol.20, no.24, 6735-.

상세보기

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