[논문]Ex VivoExpansion of Early and Late Megakaryocyte Progenitors

Lefebvre, Phil; Winter, Jane N.; Meng, Yuru; Cohen, Isaac

doi:10.1089/152581600750062363

Ex VivoExpansion of Early and Late Megakaryocyte Progenitors

Journal of hematotherapy & stem cell research, v.9 no.6, 2000년, pp.913 - 921

Lefebvre, Phil (Department of Cell and Molecular Biology, and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611.) , Winter, Jane N. (Division of Hematology) , Meng, Yuru (Department of Cell and Molecular Biology, and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611.) , Cohen, Isaac (Department of Cell and Molecular Biology, and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611.)

Abstract ▼ AI-Helper

Our goal is to produce ex vivo-expanded human megakaryocytes (MK) cells from peripheral blood progenitor cell (PBPC) harvests for use in supplementing conventional autografts. In this paper we show the megakaryocytopoietic productivity of small-scale in vitro serum-free cultures of human CD34+ cells containing MK growth and development factor (MGDF) and stem cell factor (Kit ligand; SCF) +/- granulocyte colony-stimulating factor (G-CSF). Cultures were characterized after 3, 6, 9, and 13 days by flow cytometry and clonogenic assays. CD34+ cells expanded 5.2- and 3.4-fold, and produced 2.2 and 2.4 CD34+/41(+) cells per seeded CD34+ cell after 6 and 9 days in culture, respectively. None were detected at day 13. CD41+ cells expanded exponentially over 13 days. Colony-forming unit-megakaryocyte (CFU-MK) also expanded exponentially, but the proportion of the most primitive CFU-MK dropped from 45% to 1.5% and to <1% after 6 and 9 days, respectively. G-CSF increased total cell expansion, but decreased CD41+ frequency, yielding no gain in MK production. We also found that PB CD34+ cells cultured for 3-6 days are richer in primitive MK progenitors, while those cultured for 9-13 days have greater numbers of more differentiated MKs. Overall, the combination of MGDF+SCF proved sufficient for expanding CD34+/CD41+ cells. As the stage of ex vivo MK differentiation most conducive to optimal platelet production in vivo is not known, we are planning a clinical trial to determine the efficacy of ex vivo-expanded MKs on platelet recovery in relation to MK maturity.

참고문헌 (34)

Blood Kessinger A 723 71 1988 10.1182/blood.V71.3.723.723

상세보기
Bone Marrow Transplant Williams SF 129 5 1990

상세보기
Webb, Iain J., Anderson, Kenneth C.. Risks, Costs, and Alternatives to Platelet Transfusions. Leukemia & lymphoma, vol.34, no.1, 71-84.

상세보기
Bartley, T.D., Bogenberger, J., Hunt, P., Li, Y.-S., Lu, H.S., Martin, F., Chang, M.-S., Samal, B., Nichol, J.L., Swift, S., Johnson, M.J., Hsu, R.-Y., Parker, V.P., Suggs, S., Skrine, J.D., Merewether, L.A., Clogston, C., Hsu, E., Hokom, M.M., Hornkohl, A., Choi, E., Pangelinan, M., Sun, Y., Mar, V., McNinch, J., Simonet, L., Jacobsen, F., Xie, C., Shutter, J., Chute, H., Basu, R., Selander, L., Trollinger, D., Sieu, L., Padilla, D., Trail, G., Elliott, G., Izumi, R., Covey, T., Crouse, J., Garcia, A., Xu, W., Del Castillo, J., Biron, J., Cole, S., Hu, M.C.-T., Pacifici, R., Ponting, I., Saris, C., Wen, D., Yung, Y.P., Lin, H., Rosselman, R.A.. Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor MpI. Cell, vol.77, no.7, 1117-1124.

상세보기
Blood Hokom MM 4486 86 1995 10.1182/blood.V86.12.4486.bloodjournal86124486

상세보기
Lok, Si, Foster, Donald C.. The structure, biology and potential therapeutic applications of recombinant thrombopoietin. Stem cells^®, vol.12, no.6, 586-598.

상세보기
Blood Beveridge R 580a 90 1997
Blood Bolwell B 171a 90 1997
Blood Glaspy J 580a 90 1997 10.1182/blood.V90.8.2939

상세보기
Blood Nash R 262a 90 1997
Blood Linker C 682a 92 1998
Exp Hematol Takamatsu Y 8 23 1995
Blood Dercksen MW 3771 86 1995 10.1182/blood.V86.10.3771.bloodjournal86103771

상세보기
Feng, R, Shimazaki, C, Inaba, T, Takahashi, R, Hirai, H, Kikuta, T, Sumikuma, T, Yamagata, N, Ashihara, E, Fujita, N, Nakagawa, M. CD34⁺/CD41a⁺ cells best predict platelet recovery after autologous peripheral blood stem cell transplantation. Bone marrow transplantation, vol.21, no.12, 1217-1222.

상세보기
Blood Ratajczak MZ 353 91 1998 10.1182/blood.V91.1.353

상세보기
Blood Debili N 2516 86 1995 10.1182/blood.V86.7.2516.2516

상세보기
Lefebvre, P., Winter, J. N., Rademaker, A. W., Goolsby, C., Cohen, I.. In Vitro Production of Megakaryocytes from PIXY321 versus GM‐CSF‐Mobilized Peripheral Blood Progenitor Cells. Stem cells^®, vol.15, no.2, 112-118.

상세보기
Blood Williams JL 4118 91 1998 10.1182/blood.V91.11.4118

상세보기
Blood Bertolini F 2679 89 1997 10.1182/blood.V89.8.2679

상세보기
Reiffers, Josy, Cailliot, Christian, Dazey, Bernard, Attal, Michel, Caraux, Jean, Boiron, Jean-Michel. Abrogation of post-myeloablative chemotherapy neutropenia by ex-vivo expanded autologous CD34-positive cells. The Lancet, vol.354, no.9184, 1092-1093.

상세보기
Blood McNiece I 3001 96 2000 10.1182/blood.V96.9.3001

상세보기
Miltenyi, Stefan, Müller, Werner, Weichel, Walter, Radbruch, Andreas. High gradient magnetic cell separation with MACS. Cytometry, vol.11, no.2, 231-238.

상세보기
Blood Jackson CW 768 63 1984 10.1182/blood.V63.4.768.768

상세보기
Blood Lefebvre P 647a 92 1998 10.1182/blood.V92.2.647

상세보기
Exp Hematol Koizumi K 1140 26 1998

상세보기
CANCELAS, JOSÉ ANTONIO, QUEROL, SERGIO, CANALS, CARME, BERTRAN, JOAN, LIMON, ANA, AMILL, BEGOÑA, PETRIZ, JORDI, GARCIA-LOPEZ, JOAN. CD34+ Cell Positive Selection from Mobilized Peripheral Blood by an Indirect Immunomagnetic Method: Effect of the Type of Mobilization and Assessment of Tumor Depletion Ability. Journal of hematotherapy, vol.4, no.6, 531-538.

상세보기
COLTER, MARK, JONES, MARK, HEIMFELD, SHELLY. CD34+ Progenitor Cell Selection: Clinical Transplantation, Tumor Cell Purging, Gene Therapy, Ex Vivo Expansion, and Cord Blood Processing. Journal of hematotherapy, vol.5, no.2, 179-184.

상세보기
Blood Gazitt Y 381 86 1995 10.1182/blood.V86.1.381.bloodjournal861381

상세보기
Blood Tricot G 4489 91 1998 10.1182/blood.V91.12.4489

상세보기
Blood Ebbe S 20 26 1965 10.1182/blood.V26.1.20.20

상세보기
Blood Levine RF 1122 60 1982 10.1182/blood.V60.5.1122.1122

상세보기
Blood Guerriero R 3725 86 1995 10.1182/blood.V86.10.3725.bloodjournal86103725

상세보기
Blood Choi ES 402 85 1995 10.1182/blood.V85.2.402.402

상세보기
Blood Lacombe F 347b 94 1999

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Ex VivoExpansion of Early and Late Megakaryocyte Progenitors

Abstract ▼ AI-Helper

참고문헌 (34)

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