[논문]Ex VivoExpansion of Early and Late Megakaryocyte Progenitors

Lefebvre, Phil; Winter, Jane N.; Meng, Yuru; Cohen, Isaac

doi:10.1089/152581600750062363

[해외논문] Ex VivoExpansion of Early and Late Megakaryocyte Progenitors

Journal of hematotherapy & stem cell research, v.9 no.6, 2000년, pp.913 - 921

Lefebvre, Phil (Department of Cell and Molecular Biology, and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611.) , Winter, Jane N. (Division of Hematology) , Meng, Yuru (Department of Cell and Molecular Biology, and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611.) , Cohen, Isaac (Department of Cell and Molecular Biology, and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611.)

Abstract ▼ AI-Helper

Our goal is to produce ex vivo-expanded human megakaryocytes (MK) cells from peripheral blood progenitor cell (PBPC) harvests for use in supplementing conventional autografts. In this paper we show the megakaryocytopoietic productivity of small-scale in vitro serum-free cultures of human CD34+ cells containing MK growth and development factor (MGDF) and stem cell factor (Kit ligand; SCF) +/- granulocyte colony-stimulating factor (G-CSF). Cultures were characterized after 3, 6, 9, and 13 days by flow cytometry and clonogenic assays. CD34+ cells expanded 5.2- and 3.4-fold, and produced 2.2 and 2.4 CD34+/41(+) cells per seeded CD34+ cell after 6 and 9 days in culture, respectively. None were detected at day 13. CD41+ cells expanded exponentially over 13 days. Colony-forming unit-megakaryocyte (CFU-MK) also expanded exponentially, but the proportion of the most primitive CFU-MK dropped from 45% to 1.5% and to <1% after 6 and 9 days, respectively. G-CSF increased total cell expansion, but decreased CD41+ frequency, yielding no gain in MK production. We also found that PB CD34+ cells cultured for 3-6 days are richer in primitive MK progenitors, while those cultured for 9-13 days have greater numbers of more differentiated MKs. Overall, the combination of MGDF+SCF proved sufficient for expanding CD34+/CD41+ cells. As the stage of ex vivo MK differentiation most conducive to optimal platelet production in vivo is not known, we are planning a clinical trial to determine the efficacy of ex vivo-expanded MKs on platelet recovery in relation to MK maturity.

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DOI : 10.1089/152581600750062363
OCLC ECO : 저널
Mary Ann Liebert, Inc. : 저널> 권호 > 논문

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