[논문]Phenotype analysis of cytochrome P450 2C19 in Chinese subjects with mephenytoin S/R enantiomeric ratio in urine measured by chiral GC

Yao,, T. W.; Zeng, S.; Wang, T. W.; Chen, S. Q.

doi:10.1002/bmc.19

Phenotype analysis of cytochrome P450 2C19 in Chinese subjects with mephenytoin S/R enantiomeric ratio in urine measured by chiral GC

Biomedical chromatography : BMC, v.15 no.1, 2001년, pp.9 - 13

Yao,, T. W. (College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, People's Republic of China) , Zeng, S. (College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, People's Republic of China) , Wang, T. W. (College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, People's Republic of China) , Chen, S. Q. (College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, People's Republic of China)

Abstract ▼ AI-Helper

A chiral gas chromatographic method with FID was developed for the determination of S- and R-mephenytoin in human urine. The assay is linear from 25 to 800 ng/mL for each enantiomer and the limit of detection and limit of quantitation were 12 and 25ng/mL for each enantiomer, respectively. The method affords average recoveries of 74.41 ± 3.93% and 73.78 ± 3.02% for S- and R-mephenytoin, respectively. The method allows the phenotype study of CYP2C19 in Chinese subjects. The phenotype pattern of 90 Chinese volunteers was determined, in which 26 volunteers received phenotyping and genotyping tests. The results of phenotype analysis showed that the interindividual variation was marked. The mephenytoin S/R enantiomeric ratios in urine of 11 volunteers were ≥0.95 and identified as poor metabolizers. The frequency of poor metabolizers was 12.2% in the Chinese subjects tested. A good relationship between phenotype and genotype analysis of CYP2C19 was observed. Copyright © 2001 John Wiley & Sons, Ltd.Abbreviations used:EM ; extensive metabolizer MP ; mephenytoin PM ; Poor metabolizer

참고문헌 (14)

Baumann, P., Jonzier-Perey, M.. GC and GC-MS procedures for simultaneous phenotyping with dextromethorphan and mephenytoin. Clinica chimica acta, vol.171, no.2, 211-222.

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Bertilsson, Leif, Lou, Ya-Qing, Du, Yun-Long, Liu, Yin, Kuang, Tang-Yun, Liao, Xia-Mau, Wang, Ke-Yi, Reviriego, Jesús, Iselius, Lennart, Sjöqvist, Folke. Pronounced differences between native Chinese and Swedish populations in the polymorphic hydroxylations of debrisoquin and S-mephenytoin. Clinical pharmacology and therapeutics, vol.51, no.4, 388-397.

상세보기
Molecular Pharmacology De Morais S. M. F 594 46 1994

상세보기
Journal of Biological Chemistry De Morais S. M. F 15419 269 1994 10.1016/S0021-9258(17)40694-6

상세보기
Ferguson R. J. De Morais S. M. F. Benhamou S. Bouchardy C. Blaisdell J. Wilkinson G. R. Linko P. Dayer P. andGoldstein J. A.1996.Presented at ISSX San Diego CA.
Acta Pharmaceutica Sinica Kuang T. Y 307 28 1993
Journal of Pharmacology and Experimental Therapy Kupfer A 193 218 1981
Rendic, Slobodan, Carlo, Frederick J. Di. Human Cytochrome P450 Enzymes: A Status Report Summarizing Their Reactions, Substrates, Inducers, and Inhibitors. Drug metabolism reviews, vol.29, no.1, 413-580.

상세보기
Wilkinson, G.R., Peter Guengerich, F., Branch, R.A.. Genetic polymorphism of S-mephenytoin hydroxylation. Pharmacology & therapeutics, vol.43, no.1, 53-76.

상세보기
Journal of Pharmacology and Experimental Therapy Xiao Z. S 604 281 1997
Chinese Journal of Modern Applied Pharmacy Yao T. W 37 15 1998
Acta Pharmaceutica Sinica Yao T. W 338 34 1999
Zeng, S, Mao, H.Q. Stereoselective determination of fenfluramine enantiomers in rat liver microsomal incubates. Journal of chromatography. B, Biomedical sciences and applications, vol.727, no.1, 107-112.

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10.1002/(SICI)1099-0801(199902)13:1<33::AID-BMC809>3.0.CO;2-B

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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Phenotype analysis of cytochrome P450 2C19 in Chinese subjects with mephenytoin S/R enantiomeric ratio in urine measured by chiral GC

Abstract ▼ AI-Helper

참고문헌 (14)

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