[논문]Nickel-induced proteins in human HaCaT keratinocytes: annexin II and phosphoglycerate kinase

doi:10.1016/S0300-483X(00)00369-3

[해외논문] Nickel-induced proteins in human HaCaT keratinocytes: annexin II and phosphoglycerate kinase

Toxicology, v.159 no.1/2, 2001년, pp.33 - 41

Acevedo, Fernando (National Institute for Working Life, S-17184 Solna, Sweden) , Serra, Miguel Ángel (Environment Institute, Joint Research Centre, Ispra, Italy) , Ermolli, Monica (Environment Institute, Joint Research Centre, Ispra, Italy) , Clerici, Libero (Environment Institute, Joint Research Centre, Ispra, Italy) , Vesterberg, Olof (National Institute for Working Life, S-17184 Solna, Sweden)

Abstract ▼ AI-Helper

AbstractIt has been established in previous in vitro experiments with human HaCaT keratinocytes that nickel becomes cytotoxic at concentrations higher than 100 μM and that it is accumulated mainly in the cytosolic fraction (Ermolli et al., 2000). The aim of this work was to search possible biomarkers of metal insult, i.e. nickel-binding proteins or proteins differentially expressed in the cytosolic fraction of nickel-exposed cells (up to 1 mM nickel) as compared to controls. Cytosolic proteins were studied by isoelectric focusing (IEF) and two-dimensional gel electrophoresis (2-DE). Separation by IEF revealed nickel-induced changes in the abundance of cytosolic proteins as visualised with nickel-nitrilo-triacetic-alkaline phosphatase (Ni-NTA-AP) in blots. The cytosolic fraction of cells incubated with nickel, at concentrations over 100 μM, showed nickel binding components which were absent or present in significantly lower amounts in control cells. These proteins had isoelectric points (pIs) 6.9, 7.7 and 8.5. After 2-DE silver- and protein staining significantly increased abundance of four proteins was observed. Their pI values corresponded to those of the nickel binding ones seen after IEF. A protein with pI 6.9 had a molecular weight estimated to 38 kDa, two proteins with pI around 7.7 showed molecular weights of 57 and 22 kDa, respectively and another protein with pI of 8.5 had a molecular weight of 33 kDa. The increased abundance of these components, both in IEF experiments and in 2-DE, correlated with the nickel concentration in the culture media. N-terminal amino acid sequencing and database search allowed identification of one a protein as phosphoglycerate kinase and another one as annexin II. The involvement of these proteins in cellular functions and their possible implications in the mechanism of nickel toxicity in keratinocytes are discussed. Some of these proteins may be biomarker candidates for effects of nickel exposure in human keratinocytes.

Keyword

참고문헌 (50)

Contact Dermat. Barker 26 145 1992 10.1111/j.1600-0536.1992.tb00283.x Role of keratinocytes in allergic contact dermatitis

상세보기
J. Dermatol. Sci. Bastian 6 225 1993 10.1016/0923-1811(93)90043-O Localization of annexins in normal and diseased human skin

상세보기
Philos. Trans. R. Soc. Lond. B. Biol. Sci. Blake 293 93 1981 10.1098/rstb.1981.0063 Phosphoglycerate kinase

상세보기
Anal. Biochem. Blake 136 175 1984 10.1016/0003-2697(84)90320-8 A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots

상세보기
Arch. Dermatol. Res. Boehncke 287 68 1994 10.1007/BF00370721 Differential expression of heat shock protein 70 (HSP70) and heat shock cognate protein 70 (HSC70) in human epidermis

상세보기
J. Cell Biol. Boukamp 106 761 1988 10.1083/jcb.106.3.761 Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line

상세보기
Acta Derm. Venereol. Brosin 77 26 1997 10.2340/0001555577026028 Use of XTT-assay to assess the cytotoxicity of different surfactants and metal salts in human keratinocytes (HaCaT). A feasible method for in vitro testing of skin irritants

상세보기
Toxicology Corsini 142 203 2000 10.1016/S0300-483X(99)00145-6 Epidermal cytokines in experimental contact dermatitis

상세보기
Sci. Total Environ. Costa 148 191 1994 10.1016/0048-9697(94)90396-4 Molecular mechanisms of nickel carcinogenesis

상세보기
Biochim. Biophys. Acta DalleDonne 1426 32 1999 10.1016/S0304-4165(98)00120-2 The assembly of Ni2+-actin: some peculiarities

상세보기
J. Neurol. Sci. de la Monte 128 122 1995 10.1016/0022-510X(94)00218-D Modulation of p36 gene expression in human neuronal cells

상세보기
Proc. Natl. Acad. Sci. USA Enk 89 1398 1992 10.1073/pnas.89.4.1398 Early molecular events in the induction phase of contact sensitivity

상세보기
10.1016/S0300-483X(00)00373-5 Ermolli, M., Menné, C., Pozzi, G., Serra, M.A., Clerici, L., 2000. Nickel, cobalt and chromium-induced cytotoxicity and intracellular accumulation in humna HaCaT keratinocytes. Toxicology (submitted).

상세보기
Fullerton, A., Hoelgaard, A., Menné, T., 1992. Topical nickel salts: the influence of counterion and vehicle on skin permeation and patch test response. In: Nieboer, E., Nriagu, J.O. (Eds.), Nickel and Human Health - Current Perspectives, Vol. 25 in the Wiley Series in Advance in Environmental Sciences and Technology - Jerome O. Nriagu Series Editor. Wiley, New York, pp. 211-222.
Mol. Toxicol. Gajjar 1 513 1987 Irritancy testing in cultured keratinocytes
J. Invest. Dermatol. Goebeler 100 759 1993 10.1111/1523-1747.ep12476328 Nickel chloride and cobalt chloride, two common contact sensitizers, directly induce expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial leukocyte adhesion molecule (ELAM-1) by endothelial cells

상세보기
Am. J. Contact Dermat. Grabbe 7 238 1996 10.1016/S1046-199X(96)90057-3 Immunoregulatory mechanisms involved in elicitation of allergic contact hypersensitivity

상세보기
J. Clin. Invest. Grabbe 98 1158 1996 10.1172/JCI118899 Dissection of antigenic and irritative effects of epicutaneously applied haptens in mice. Evidence that not the antigenic component but nonspecific proinflammatory effects of haptens determine the concentration-dependent elicitation of allergic contact dermatitis

상세보기
Contact Dermatitis Kimber 23 325 1990 10.1111/j.1600-0536.1990.tb05166.x Contact sensitization of mice to nickel sulphate and potassium dichromate

상세보기
Eur. J. Biochem. Larsson-Raznikiewicz 17 183 1970 10.1111/j.1432-1033.1970.tb01152.x The phosphoglycerate kinase reaction and its metal ion specificity

상세보기
Biochem. Biophys. Res. Commun. Lee 258 592 1999 10.1006/bbrc.1999.0692 Nickel(II) acetate-treated Chinese hamster ovary cells differentially express Vimentin, hSNF2H homologue, and H ferritin

상세보기
J. Biol. Chem. Li 271 21262 1996 10.1074/jbc.271.35.21262 Induction of phosphoglycerate kinase 1 gene expression by hypoxia. Roles of Arnt and HIF1alpha

상세보기
Res. Commun. Chem. Pathol. Pharmacol. Lin 65 275 1989 Protein blotting method for detection of nickel-binding proteins
Int. Immunol. Lisby 7 343 1995 10.1093/intimm/7.3.343 Nickel and skin irritants up-regulate tumor necrosis factor-alpha mRNA in keratinocytes by different but potentially synergistic mechanisms

상세보기
Br. J. Dermatol. Little 134 199 1996 10.1111/j.1365-2133.1996.tb07602.x Chromium- and nickel-induced cytotoxicity in normal and transformed human keratinocytes: an investigation of pharmacological approaches to the prevention of Cr(VI)-induced cytotoxicity

상세보기
Br. J. Dermatol. Little 138 45 1998 10.1046/j.1365-2133.1998.02025.x The participation of proliferative keratinocytes in the preimmune response to sensitizing agents

상세보기
Arch. Dermatol. Res. Ma 288 596 1996 10.1007/BF02505262 Annexins I and II show differences in subcellular localization and differentiation-related changes in human epidermal keratinocytes

상세보기
J. Cell Sci. Ma 107 1973 1994 10.1242/jcs.107.7.1973 Immunocytochemical detection of extracellular annexin II in cultured human skin keratinocytes and isolation of annexin II isoforms enriched in the extracellular pool

상세보기
Dermatol. Clin. McKenzie 8 649 1990 10.1016/S0733-8635(18)30452-2 Keratinocyte cytokines and growth factors. Functions in skin immunity and homeostasis

상세보기
Menné, T., 1992. Nickel contact hypersensitivity. In: Nieboer, E., Nriagu, J.O. (Eds.), Nickel and Human Health - Current Perspectives, Vol. 25 in the Wiley Series in Advance in Environmental Sciences and Technology - Jerome O. Nriagu Series Editor. Wiley, New York, pp. 193-200.
Ann. Clin. Lab. Sci. Menné 26 133 1996 Prevention of nickel allergy by regulation of specific exposures

상세보기
Nicklin, S., Nielsen, G.D., 1992. Nickel and the Immune System: Current Concepts. In: Nieboer, E., Nriagu, J.O. (Eds.), Nickel and Human Health - Current Perspectives, Vol. 25 in the Wiley Series in Advance in Environmental Sciences and Technology - Jerome O. Nriagu Series Editor. Wiley, New York, pp. 239-260.
J. Invest. Dermatol. Munz 108 307 1997 10.1111/1523-1747.ep12286470 Differential expression of the calpactin I subunits annexin II and p11 in cultured keratinocytes and during wound repair

상세보기
J. Cell Physiol. Murata 180 105 1999 10.1002/(SICI)1097-4652(199907)180:1<105::AID-JCP12>3.0.CO;2-5 Differential metal response and regulation of human heavy metal-inducible genes

상세보기
Electrophoresis Nielsen 15 666 1994 10.1002/elps.1150150194 Studies of serum protein complexes with nickel using crossed immunoelectrophoresis

상세보기
Ann. Clin. Lab. Sci. Oskarsson 9 47 1979 An autoradiographic study on the distribution of 63NiCl2 in mice

상세보기
Skin Pharmacol. Pape 4 205 1991 10.1159/000210950 In vitro methods for the assessment of primary local effects of topically applied preparations

상세보기
Br. J. Dermatol. Picardo 122 729 1990 10.1111/j.1365-2133.1990.tb06259.x Nickel-keratinocyte interaction: a possible role in sensitization

상세보기
Biochim. Biophys. Acta Raynal 1197 63 1994 10.1016/0304-4157(94)90019-1 Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins

상세보기
Acta Derm. Venereol. Sainte-Marie 78 169 1998 10.1080/000155598441459 Comparative study of the in vitro inflammatory activity of three nickel salts on keratinocytes

상세보기
Toxicol. Appl. Pharmacol. Salnikow 160 127 1999 10.1006/taap.1999.8759 Role of Ca(2+) in the regulation of nickel-inducible Cap43 gene expression

상세보기
Cancer Res. Salnikow 60 38 2000 Carcinogenic nickel induces genes involved with hypoxic stress

상세보기
Scheynius 4 1998 Allergic Contact Dermatitis - The Molecular Basis Immunological aspects
J. Biol. Chem. Semenza 269 23757 1994 10.1016/S0021-9258(17)31580-6 Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1

상세보기
Carcinogenesis Shiao 19 1203 1998 10.1093/carcin/19.7.1203 Cell cycle arrest, apoptosis and p53 expression in nickel(II) acetate-treated Chinese hamster ovary cells

상세보기
J. Virol. Sisk 68 766 1994 10.1128/JVI.68.2.766-775.1994 High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells

상세보기
Gene Vellucci 166 213 1995 10.1016/0378-1119(95)00543-9 Cloning of putative growth regulatory genes from primary human keratinocytes by subtractive hybridization

상세보기
Biochim. Biophys. Acta Walsh 999 64 1989 10.1016/0167-4838(89)90031-9 Glycolytic enzyme interactions with tubulin and microtubules

상세보기
Westermeier 1997 Electrophoresis in Practice
Cancer Res. Zhou 58 2182 1998 Cap43, a novel gene specifically induced by Ni2+ compounds

상세보기

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증