[논문]Identification, Purification, and Characterization of Iminodiacetate Oxidase from the EDTA-Degrading Bacterium BNC1

Liu, Yong; Louie, Tai Man; Payne, Jason; Bohuslavek, Jan; Bolton Jr., Harvey; Xun, Luying

doi:10.1128/aem.67.2.696-701.2001

[해외논문] Identification, Purification, and Characterization of Iminodiacetate Oxidase from the EDTA-Degrading Bacterium BNC1 원문보기

Applied and environmental microbiology, v.67 no.2, 2001년, pp.696 - 701

Liu, Yong (<) , Louie, Tai Man (!--label omitted: 1-->) , Payne, Jason (School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234,1 and) , Bohuslavek, Jan (<) , Bolton Jr., Harvey (!--label omitted: 1-->) , Xun, Luying (School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234,1 and)

Abstract ▼ AI-Helper

ABSTRACT Microbial degradation of synthetic chelating agents, such as EDTA and nitrilotriacetate (NTA), may help immobilizing radionuclides and heavy metals in the environment. The EDTA- and NTA-degrading bacterium BNC1 uses EDTA monooxygenase to oxidize NTA to iminodiacetate (IDA) and EDTA to ethylenediaminediacetate (EDDA). IDA- and EDDA-degrading enzymes have not been purified and characterized to date. In this report, an IDA oxidase was purified to apparent homogeneity from strain BNC1 by using a combination of eight purification steps. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a single protein band of 40 kDa, and by using size exclusion chromatography, we estimated the native enzyme to be a homodimer. Flavin adenine dinucleotide was determined as its prosthetic group. The purified enzyme oxidized IDA to glycine and glyoxylate with the consumption of O 2 . The temperature and pH optima for IDA oxidation were 35°C and 8, respectively. The apparent K m for IDA was 4.0 mM with a kcat of 5.3 s −1 . When the N-terminal amino acid sequence was determined, it matched exactly with that encoded by a previously sequenced hypothetical oxidase gene of BNC1. The gene was expressed in Escherichia coli , and the gene product as a C-terminal fusion with a His tag was purified by a one-step nickel affinity chromatography. The purified fusion protein had essentially the same enzymatic activity and properties as the native IDA oxidase. IDA oxidase also oxidized EDDA to ethylenediamine and glyoxylate. Thus, IDA oxidase is likely the second enzyme in both NTA and EDTA degradation pathways in strain BNC1.

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