[논문]The Green Fluorescent Protein Gene Functions as a Reporter of Gene Expression in Phanerochaete chrysosporium

Ma, Biao; Mayfield, Mary B.; Gold, Michael H.

doi:10.1128/aem.67.2.948-955.2001

The Green Fluorescent Protein Gene Functions as a Reporter of Gene Expression in Phanerochaete chrysosporium 원문보기

Applied and environmental microbiology, v.67 no.2, 2001년, pp.948 - 955

Ma, Biao (<) , Mayfield, Mary B. (!--label omitted: 1-->) , Gold, Michael H. (Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, Beaverton, Oregon 97006-8921)

Abstract ▼ AI-Helper

ABSTRACT The enhanced green fluorescent protein (GFP) gene ( egfp ) was used as a reporter of gene expression driven by the glyceraldehyde- p -dehydrogenase ( gpd ) gene promoter and the manganese peroxidase isozyme 1 ( mnp1 ) gene promoter in Phanerochaete chrysosporium . Four different constructs were prepared. pUGGM3′ and pUGiGM3′ contain the P. chrysosporium gpd promoter fused upstream of the egfp coding region, and pUMGM3′ and pUMiGM3′ contain the P. chrysosporium mnp1 promoter fused upstream of the egfp gene. In all constructs, the egfp gene was followed by the mnp1 gene 3′ untranslated region. In pUGGM3′ and pUMGM3′, the promoters were fused directly with egfp , whereas in pUGiGM3′ and pUMiGM3′, following the promoters, the first exon (6 bp), the first intron (55 bp), and part of the second exon (9 bp) of the gpd gene were inserted at the 5′ end of the egfp gene. All constructs were ligated into a plasmid containing the ura1 gene of Schizophyllum commune as a selectable marker and were used to transform a Ural1 auxotrophic strain of P. chrysosporium to prototrophy. Crude cell extracts were examined for GFP fluorescence, and where appropriate, the extracellular fluid was examined for MnP activity. The transformants containing a construct with an intron 5′ of the egfp gene (pUGiGM3′ and pUMiGM3′) exhibited maximal fluorescence under the appropriate conditions. The transformants containing constructs with no introns exhibited minimal or no fluorescence. Northern (RNA) blots indicated that the insertion of a 5′ intron resulted in more egfp RNA than was found in transformants carrying an intronless egfp . These results suggest that the presence of a 5′ intron affects the expression of the egfp gene in P. chrysosporium . The expression of GFP in the transformants carrying pUMiGM3′ paralled the expression of endogenous mnp with respect to nitrogen and Mn levels, suggesting that this construct will be useful in studying cis -acting elements in the mnp1 gene promoter.

참고문헌 (51)

Akileswaran, Lakshmi, Alic, Margaret, Clark, Edith K., Hornick, Jason L., Gold, Michael H.. Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Current genetics, vol.23, no.4, 351-356.

상세보기
Alic, Margaret, Akileswaran, Lakshmi, Gold, Michael H. Characterization of the gene encoding manganese peroxidase isozyme 3 from Phanerochaete chrysosporium. Biochimica et biophysica acta, Protein structure and molecular enzymology, vol.1338, no.1, 1-7.

상세보기
Alic, Margaret, Kornegay, Janet R., Pribnow, David, Gold, Michael H.. Transformation by Complementation of an Adenine Auxotroph of the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Applied and environmental microbiology, vol.55, no.2, 406-411.

상세보기
Alic, Margaret, Letzring, Celia, Gold, Michael H.. Mating System and Basidiospore Formation in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Applied and environmental microbiology, vol.53, no.7, 1464-1469.

상세보기
Alic, Margaret, Mayfield, Mary B., Akileswaran, Lakshmi, Gold, Michael H.. Homologous transformation of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Current genetics, vol.19, no.6, 491-494.

상세보기
Bao, W., Fukushima, Y., Jensen, K.A., Moen, M.A., Hammel, K.E.. Oxidative degradation of non-phenolic lignin during lipid peroxidation by fungal manganese peroxidase. FEBS letters, vol.354, no.3, 297-300.

상세보기
Brown, J A, Alic, M, Gold, M H. Manganese peroxidase gene transcription in Phanerochaete chrysosporium: activation by manganese. Journal of bacteriology, vol.173, no.13, 4101-4106.

상세보기
Brown, J A, Glenn, J K, Gold, M H. Manganese regulates expression of manganese peroxidase by Phanerochaete chrysosporium. Journal of bacteriology, vol.172, no.6, 3125-3130.

상세보기
Brown, Julie A., Li, Dan, Alic, Margaret, Gold, Michael H.. Heat Shock Induction of Manganese Peroxidase Gene Transcription in Phanerochaete chrysosporium. Applied and environmental microbiology, vol.59, no.12, 4295-4299.

상세보기
Bumpus, John A., Aust, Steven D.. Biodegradation of environmental pollutants by the white rot fungus Phanerochaete chrysosporium: Involvement of the lignin degrading system. BioEssays, vol.6, no.4, 166-170.

상세보기
Cormack, Brendan. Green fluorescent protein as a reporter of transcription and protein localization in fungi. Current opinion in microbiology, vol.1, no.4, 406-410.

상세보기
Cubitt, Andrew B., Heim, Roger, Adams, Stephen R., Boyd, Aileen E., Gross, Larry A., Tsien, Roger Y.. Understanding, improving and using green fluorescent proteins. Trends in biochemical sciences, vol.20, no.11, 448-455.

상세보기
Cullen, Daniel. Recent advances on the molecular genetics of ligninolytic fungi. Journal of biotechnology, vol.53, no.2, 273-289.

상세보기
10.1007/978-3-642-46687-8 Eriksson K.- E. L. Blanchette R. A. Ander P. Microbial and enzymatic degradation of wood and wood components. 1990 Springer-Verlag Berlin Germany
Gettemy, Jessica M., Ma, Biao, Alic, Margaret, Gold, Michael H.. Reverse Transcription-PCR Analysis of the Regulation of the Manganese Peroxidase Gene Family. Applied and environmental microbiology, vol.64, no.2, 569-574.

상세보기
Glenn, Jeffrey K., Gold, Michael H.. Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Archives of biochemistry and biophysics, vol.242, no.2, 329-341.

상세보기
Godfrey, B J, Akileswaran, L, Gold, M H. A reporter gene construct for studying the regulation of manganese peroxidase gene expression. Applied and environmental microbiology, vol.60, no.4, 1353-1358.

상세보기
Godfrey, B.J., Mayfield, M.B., Brown, J.A., Gold, M.H.. Characterization of a gene encoding a manganese peroxidase from Phanerochaete chrysosporium. Gene, vol.93, no.1, 119-124.

상세보기
Gold, M H, Alic, M. Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Microbiological reviews, vol.57, no.3, 605-622.

상세보기
10.1021/bk-1989-0389.ch009
Met. Ions Biol. Syst. Gold M. H. 558 37 1999 Manganese peroxidases. Gold M. H. Youngs H. L. Sollewijn Gelpke M. D. Manganese peroxidases. Met. Ions Biol. Syst. 37 1999 558 586
Met. Ions Biol. syst. Hammel K. E. 41 28 1992 Oxidation of aromatic pollutants by lignin-degrading fungi and their extracellular peroxidases. Hammel K. E. Oxidation of aromatic pollutants by lignin-degrading fungi and their extracellular peroxidases. Met. Ions Biol. syst. 28 1992 41 60
Harmsen, Martin C., Schuren, Frank H. J., Moukha, Serge M., van Zuilen, Carin M., Punt, Peter J., Wessels, Joseph G. H.. Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus. Current genetics, vol.22, no.6, 447-454.

상세보기
Harvey, P.J., Schoemaker, H.E., Bowen, R.M., Palmer, J.M.. Single-electron transfer processes and the reaction mechanism of enzymic degradation of lignin. FEBS letters, vol.183, no.1, 13-16.

상세보기
10.1093/nar/16.21.9893
Henriksen, Anne L. Santerre, Even, Sergine, Müller, Christian, Punt, Peter J., van den Hondel, Cees A. M. J. J., Nielsen, Jens. Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein. Microbiology, vol.145, no.3, 729-734.

상세보기
Huang, M T, Gorman, C M. Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA.. Nucleic acids research, vol.18, no.4, 937-947.

상세보기
Kersten, P J, Kirk, T K. Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium. Journal of bacteriology, vol.169, no.5, 2195-2201.

상세보기
Kersten, P J, Tien, M, Kalyanaraman, B, Kirk, T K. The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes.. The Journal of biological chemistry, vol.260, no.5, 2609-2612.

상세보기
Kirk, T K, Farrell, R L. Enzymatic "Combustion": The Microbial Degradation of Lignin. Annual review of microbiology, vol.41, 465-501.

상세보기
Kirk, T. K., Schultz, E., Connors, W. J., Lorenz, L. F., Zeikus, J. G.. Influence of culture parameters on lignin metabolism byPhanerochaete chrysosporium. Archives of microbiology, vol.117, no.3, 277-285.

상세보기
Li, D, Alic, M, Brown, J A, Gold, M H. Regulation of manganese peroxidase gene transcription by hydrogen peroxide, chemical stress, and molecular oxygen. Applied and environmental microbiology, vol.61, no.1, 341-345.

상세보기
Li, D, Alic, M, Gold, M H. Nitrogen regulation of lignin peroxidase gene transcription. Applied and environmental microbiology, vol.60, no.9, 3447-3449.

상세보기
Lugones, Luis G., Scholtmeijer, Karin, Klootwijk, Riko, Wessels, Joseph G. H.. Introns are necessary for mRNA accumulation in Schizophyllum commune. Molecular microbiology, vol.32, no.4, 681-689.

상세보기
Matsumoto, Ken, Wassarman, Karen Montzka, Wolffe, Alan P.. Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA. The EMBO journal, vol.17, no.7, 2107-2121.

상세보기
Mayfield, M B, Kishi, K, Alic, M, Gold, M H. Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium. Applied and environmental microbiology, vol.60, no.12, 4303-4309.

상세보기
Naylor, Louise H. Reporter gene technology: the future looks bright. Biochemical pharmacology, vol.58, no.5, 749-757.

상세보기
Pease, E A, Andrawis, A, Tien, M. Manganese-dependent Peroxidase from Phanerochaete chrysosporium. The Journal of biological chemistry, vol.264, no.23, 13531-13535.

상세보기
Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G., Cormier, M.J.. Primary structure of the Aequorea victoria green-fluorescent protein. Gene, vol.111, no.2, 229-233.

상세보기
Pribnow, D, Mayfield, M B, Nipper, V J, Brown, J A, Gold, M H. Characterization of a cDNA Encoding a Manganese Peroxidase, from the Lignin-degrading Basidiomycete Phanerochaete Chrysosporium. The Journal of biological chemistry, vol.264, no.9, 5036-5040.

상세보기
Raeder, Ute, Broda, Paul. Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level. Current genetics, vol.8, no.7, 499-506.

상세보기
Reddy, G.B., Gold, M.H.. A Two-Component Tetrachlorohydroquinone Reductive Dehalogenase System from the Lignin-Degrading BasidiomycetePhanerochaete chrysosporium. Biochemical and biophysical research communications, vol.257, no.3, 901-905.

상세보기
Reddy, G. Vijay Bhasker, Sollewijn Gelpke, Maarten D., Gold, Michael H.. Degradation of 2,4,6-Trichlorophenol by Phanerochaete chrysosporium : Involvement of Reductive Dechlorination. Journal of bacteriology, vol.180, no.19, 5159-5164.

상세보기
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., Klenk, D.C.. Measurement of protein using bicinchoninic acid. Analytical biochemistry, vol.150, no.1, 76-85.

상세보기
Sollewijn Gelpke, Maarten D., Mayfield-Gambill, Mary, Lin Cereghino, Geoffrey P., Gold, Michael H.. Homologous Expression of Recombinant Lignin Peroxidase in Phanerochaete chrysosporium. Applied and environmental microbiology, vol.65, no.4, 1670-1674.

상세보기
Mol. Gen. Genet. Spellig T. 503 252 1996 Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Spellig T. Bottin A. Kahmann R. Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Mol. Gen. Genet. 252 1996 503 509

상세보기
Sundaramoorthy, M, Kishi, K, Gold, M H, Poulos, T L. The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution. The Journal of biological chemistry, vol.269, no.52, 32759-32767.

상세보기
Sundaramoorthy, Munirathinam, Kishi, Katsuyuki, Gold, Michael H., Poulos, Thomas L.. Crystal Structures of Substrate Binding Site Mutants of Manganese Peroxidase. The Journal of biological chemistry, vol.272, no.28, 17574-17580.

상세보기
Tien, Ming, Tu, Chen-Pei D.. Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporium. Nature, vol.326, no.6112, 520-523.

상세보기
Wariishi, H, Valli, K, Renganathan, V, Gold, M H. Thiol-mediated Oxidation of Nonphenolic Lignin Model Compounds by Manganese Peroxidase of Phanerochaete chrysosporium. The Journal of biological chemistry, vol.264, no.24, 14185-14191.

상세보기
Wariishi, H, Valli, K, Gold, M.H.. Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators.. The Journal of biological chemistry, vol.267, no.33, 23688-23695.

상세보기

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The Green Fluorescent Protein Gene Functions as a Reporter of Gene Expression in Phanerochaete chrysosporium 원문보기

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