[논문]Characterization of grouper nervous necrosis virus (GNNV)

Chi, S C; Lo, B J; Lin, S C

doi:10.1046/j.1365-2761.2001.00256.x

[해외논문] Characterization of grouper nervous necrosis virus (GNNV) 원문보기

Journal of fish diseases, v.24 no.1, 2001년, pp.3 - 13

Chi, S C , Lo, B J , Lin, S C

Abstract ▼ AI-Helper

Grouper nervous necrosis virus (GNNV) was isolated from moribund grouper larvae, Epinephelus sp., using a fish cell line GF‐1. The present study describes the biochemical and biophysical properties of GNNV and the expression of GNNV in diseased grouper larvae. Viral protein was detectable in most of the GNNV‐infected GF‐1 cells by the fluorescent antibody technique (FAT) after 12 h post‐infection (p.i.), although no cytopathic effect (CPE) appeared at that time. Clear CPE developed on the third day, and complete disintegration of the monolayer occurred over the subsequent two days. The infectivity of GNNV can be blocked following treatment at 60 °C for 1 h. GNNV was sensitive to pH 3 and pH 10-12 with a 4 log10 drop in infectivity. Purified GNNV was analysed by SDS-PAGE, and then stained with periodic acid silver. The positive staining indicated that its two capsid proteins were glycoproteins. Genomic RNAs of GNNV were extracted from purified virions and analysed. The molecular weights of genomic RNAs were 1.02 × 106 and 0.50 × 106 Da. The T2 region of the coat protein gene of GNNV was amplified by polymerase chain reaction (PCR), and the multiple alignment of the T2 sequence of two GNNV isolates with four genotypes of fish nodaviruses revealed that these two isolates (GNNV9410 and GNNV9508) belong to the red‐spotted grouper nervous necrosis virus (RGNNV) genotype. The tissue distribution of GNNV in naturally infected grouper larvae was investigated by in situ hybridization using a dig‐labelled probe, which showed that GNNV was not only detected in the brain and retina, but also in the gill, skeletal muscle, liver, pyloric gland, intestine and blood cells in the heart.

참고문헌 (20)

Arimoto, Misao, Sato, Jun, Maruyama, Keigo, Mimura, Gen, Furusawa, Iwao. Effect of chemical and physical treatments on the inactivation of striped jack nervous necrosis virus (SJNNV). Aquaculture, vol.143, no.1, 15-22.

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Le Breton, A, Grisez, L, Sweetman, J, Ollevier, F. Viral nervous necrosis (VNN) associated with mass mortalities in cage‐reared sea bass,Dicentrarchus labrax (L.). Journal of fish diseases, vol.20, no.2, 145-151.

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CAPD Fisheries Series no. 18, Reports on Fish Disease Research Chi S.C. 59 18 1997 The investigation of viral disease among cultured groupers in southern Taiwan
Chi, S C, Lo, C F, Kou, G H, Chang, P S, Peng, S E, Chen, S N. Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery‐reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel). Journal of fish diseases, vol.20, no.3, 185-193.

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ChiS.C.&LoB.J.(1998)Preliminary investigation of the infection pathway of grouper nervous necrosis virus.Research and Application of Biotechnology in Aquaculture 156-166.
Decostere, A., Haesebrouck, F., Turnbull, J. F., Charlier, G.. Influence of water quality and temperature on adhesion of high and low virulence Flavobacterium columnare strains to isolated gill arches. Journal of fish diseases, vol.22, no.1, 1-11.

상세보기
Chi, Shau-Chi, Lin, Su-Ching, Su, Huei-Meei, Hu, Wei-Wen. Temperature effect on nervous necrosis virus infection in grouper cell line and in grouper larvae. Virus research : an international journal of molecular and cellular virology, vol.63, no.1, 107-114.

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Comps, M., Trindade, M., Delsert, Cl.. Investigation of fish encephalitis viruses (FEV) expression in marine fishes using DIG-labelled probes. Aquaculture, vol.143, no.2, 113-121.

상세보기
Dubray, Gérard, Bezard, Guy. A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. Analytical biochemistry, vol.119, no.2, 325-329.

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Frerichs, G. N., Rodger, H. D., Peric, Z.. Cell Culture Isolation of Piscine Neuropathy Nodavirus from Juvenile Sea Bass, Dicentrarchus labrax. The Journal of general virology, vol.77, no.9, 2067-2071.

상세보기
Grotmol, S, Bergh, Ø, Totland, GK. Transmission of viral encephalopathy and retinopathy (VER) to yolk-sac larvae of the Atlantic halibut Hippoglossus hippoglossus:occurrence of nodavirus in various organs and a possible route of infection. Diseases of aquatic organisms, vol.36, 95-106.

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Grotmol, S, Totland, GK, Thorud, K, Hjeltnes, BK. Vacuolating encephalopathy and retinopathy associated with a nodavirus-like agent:a probable cause of mass mortality of cultured larval and juvenile Atlantic halibut Hippoglossus hippoglossus. Diseases of aquatic organisms, vol.29, 85-97.

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Mori, K.-I., Nakai, T., Muroga, K., Arimoto, M., Mushiake, K., Furusawa, I.. Properties of a new virus belonging to nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis. Virology, vol.187, no.1, 368-371.

상세보기
Munday, B.L., Langdon, J.S., Hyatt, A., Humphrey, J.D.. Mass mortality associated with a viral-induced vacuolating encephalopathy and retinopathy of larval and juvenile barramundi, Lates calcarifer Bloch. Aquaculture, vol.103, no.3, 197-211.

상세보기
Munday, B. L., Nakai, T.. Special topic review: Nodaviruses as pathogens in larval and juvenile marine finfish. World journal of microbiology & biotechnology, vol.13, no.4, 375-381.

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Proceedings of the International Symposium on Biotechnology in Aquaculture. Asian Fisheries Society Special Publication Nakai T. 147 10 1995 Viral nervous necrosis of larval and juvenile marine fish
Nguyen, HD, Nakai, T, Muroga, K. Progression of striped jack nervous necrosis virus (SJNNV) infection in naturally and experimentally infected striped jack Pseudocaranx dentex larvae. Diseases of aquatic organisms, vol.24, 99-105.

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Nishizawa, T., Mori, K.-i., Furuhashi, M., Nakai, T., Furusawa, I., Muroga, K.. Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. The Journal of general virology, vol.76, no.7, 1563-1569.

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Nishizawa, T, Mori, K, Nakai, T, Furusawa, I, Muroga, K. Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Diseases of aquatic organisms, vol.18, 103-107.

상세보기
Nishizawa, T, Furuhashi, M, Nagai, T, Nakai, T, Muroga, K. Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Applied and environmental microbiology, vol.63, no.4, 1633-1636.

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DOI : 10.1046/j.1365-2761.2001.00256.x
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