Gardner, T.A.
(University of Virginia Health Sciences Center, Charlottesville, VA)
,
Ko, S.-C.
(University of Virginia Health Sciences Center, Charlottesville, VA)
,
Yang, L.
(University of Virginia Health Sciences Center, Charlottesville, VA)
,
Cadwell, J.J.S.
(FiberCell Systems, New Market, MD, USA)
,
Chung, L.W.K.
(University of Virginia Health Sciences Center, Charlottesville, VA)
,
Kao, C.
(University of Virginia Health Sciences Center, Charlottesville, VA)
A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are requ...
A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.
A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.
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