[논문]On-site extraction and purification of bacterial nucleic acids from blood samples using an unpowered microfluidic device

Kim, Chi Hyun; Park, Juhwan; Kim, Soo Jee; Ko, Dae-Hyun; Lee, Song Ha; Lee, Seok Jae; Park, Je-Kyun; Lee, Moon-Keun

doi:10.1016/j.snb.2020.128346

[해외논문] On-site extraction and purification of bacterial nucleic acids from blood samples using an unpowered microfluidic device

Sensors and actuators. B, Chemical, v.320, 2020년, pp.128346 -

Kim, Chi Hyun (Nanobio Application Team, National Nanofab Center (NNFC)) , Park, Juhwan (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Kim, Soo Jee (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Ko, Dae-Hyun (Department of Laboratory Medicine, University of Ulsan and Asan Medical Center) , Lee, Song Ha (Nanobio Application Team, National Nanofab Center (NNFC)) , Lee, Seok Jae (Nanobio Application Team, National Nanofab Center (NNFC)) , Park, Je-Kyun (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Lee, Moon-Keun (Nanobio Application Team, National Nanofab Center (NNFC))

Abstract ▼ AI-Helper

Abstract Molecular diagnostics is a powerful technique for detecting pathogens with high sensitivity, but it has complicated sample preparation procedures that rely not only on a manual operation by experts but also on bulky equipment. Here, we report the use of an unpowered, microfluidic sample preparation device in on-site molecular diagnosis without the necessity of expertise and electrical devices. The device is assembled with two units, a plasma separation chip, and a nucleic acid extraction and purification chip. As a model pathogen, Escherichia coli (E. coli) O157:H7 was first separated from the blood sample using the unpowered plasma separation chip, and then the DNA was extracted and purified from the separated bacteria by using the finter-actuated nucleic acid extraction and purification chip. 103 to 108 CFU/mL of E. coli O157:H7 was infiltered from the blood sample with ∼1% of permeation efficiency. The extracted DNA was successfully purified and confirmed by gel electrophoresis after the polymerase chain reaction. This unpowered sample pretreatment method is precise and reliable, even at low concentrations of down to 103 CFU/mL of E. coli in the blood sample. Highlights Unpowered sample pretreatment method was devised based on the membrane filter and the finger-actuated microfluidic unit. Using unpowered plasma separation chip, the plasma was separated and collected successfully from blood sample without electrical device. The entire nucleic acid extractionwas conducted simply by pressing the buttons on unpowered nucleic acid extraction chip. A manual sample preparation method was efficient even at low concentrations of 103 CFU/mL of E. coli in the blood samples. Graphical abstract [DISPLAY OMISSION]

Keyword

참고문헌 (38)

Landsverk 19 2013 Next Generation Sequencing: Translation to Clinical Diagnostics Clinical molecular diagnostic techniques: a brief review
Annu. Rev. Med. Tsalik 69 379 2018 10.1146/annurev-med-052716-030320 New molecular diagnostic approaches to bacterial infections and antibacterial resistance

상세보기
Biomed Res. Int. Ali 2017 10.1155/2017/9306564 Current nucleic acid extraction methods and their implications to point-of-care diagnostics

상세보기
Drug Discov. Today Rahman 17 1199 2012 10.1016/j.drudis.2012.07.001 Nucleic acid sample preparation for in vitro molecular diagnosis: from conventional techniques to biotechnology

상세보기
Thatcher 35 2018 Principles and Applications of Molecular Diagnostics Nucleic acid isolation
Lab Chip Price 9 2484 2009 10.1039/b907652m Nucleic acid extraction techniques and application to the microchip

상세보기
PLoS One Katevatis 12 2017 10.1371/journal.pone.0176848 Low concentration DNA extraction and recovery using a silica solid phase

상세보기
Angew. Chem. Int. Ed. Reinholt 53 13988 2014 10.1002/anie.201309580 Microfluidic isolation of nucleic acids

상세보기
Chem. Rev. Zhao 115 12491 2015 10.1021/acs.chemrev.5b00428 Isothermal amplification of nucleic acids

상세보기
Anal. Bioanal. Chem. Martzy 411 1695 2019 10.1007/s00216-018-1553-1 Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis

상세보기
Lab Chip Shin 14 359 2014 10.1039/C3LC51035B Solid phase nucleic acid extraction technique in a microfluidic chip using a novel non-chaotropic agent: dimethyl adipimidate

상세보기
Lab Chip Jung 13 3383 2013 10.1039/c3lc50266j A microbead-incorporated centrifugal sample pretreatment microdevice

상세보기
Lab Chip Brassard 19 1941 2019 10.1039/C9LC00276F Extraction of nucleic acids from blood: unveiling the potential of active pneumatic pumping in centrifugal microfluidics for integration and automation of sample preparation processes

상세보기
Lab Chip Campos 18 3459 2018 10.1039/C8LC00716K Microfluidic-based solid phase extraction of cell free DNA

상세보기
Lab Chip den Dulk 13 106 2013 10.1039/C2LC40929A Magneto-capillary valve for integrated purification and enrichment of nucleic acids and proteins

상세보기
Lab Chip Tian 18 3436 2018 10.1039/C8LC00700D Label-free isolation of rare tumor cells from untreated whole blood by interfacial viscoelastic microfluidics

상세보기
Anal. Chem. Zhang 94 106 2017 Point-of-care-testing of nucleic acids by microfluidics, TrAC-Trend
Lab Chip Oh 16 1917 2016 10.1039/C6LC00326E Fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device

상세보기
Sens. Actuators B Chem. Liu 270 371 2018 10.1016/j.snb.2018.05.044 A sample-to-answer labdisc platform integrated novel membrane-resistance valves for detection of highly pathogenic avian influenza viruses

상세보기
Anal. Chem. Wu 83 3336 2011 10.1021/ac103129e Integrated glass microdevice for nucleic acid purification, loop-mediated isothermal amplification, and online detection

상세보기
Sens. Actuators B Chem. Chen 283 472 2019 10.1016/j.snb.2018.12.060 Fully integrated nucleic acid pretreatment, amplification, and detection on a paper-chip for identifying EGFR mutations in lung cancer cells

상세보기
Anal. Chem. Rodriguez 87 7872 2015 10.1021/acs.analchem.5b01594 Paper-based RNA extraction, in situ isothermal amplification, and lateral flow detection for low-cost, rapid diagnosis of Influenza A (H1N1) from clinical specimens

상세보기
Lab Chip Rodriguez 16 753 2016 10.1039/C5LC01392E A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples

상세보기
Lab Chip Berry 11 1747 2011 10.1039/c1lc00004g One-step purification of nucleic acid for gene expression analysis via immiscible filtration assisted by surface tension (IFAST)

상세보기
Lab Chip Mosley 16 2108 2016 10.1039/C6LC00228E Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

상세보기
Lab Chip Olanrewaju 18 2323 2018 10.1039/C8LC00458G Capillary microfluidics in microchannels: from microfluidic networks to capillaric circuits

상세보기
Lab Chip Xu 15 3962 2015 10.1039/C5LC00716J Vacuum-driven power-free microfluidics utilizing the gas solubility or permeability of polydimethylsiloxane (PDMS)

상세보기
PLoS One Korir 10 2015 10.1371/journal.pone.0115993 Punch card programmable microfluidics

상세보기
Lab Chip Zhang 18 610 2018 10.1039/C7LC01234A Hand-powered centrifugal microfluidic platform inspired by the spinning top for sample-to-answer diagnostics of nucleic acids

상세보기
Lab Chip Iwai 14 3790 2014 10.1039/C4LC00500G Finger-powered microfluidic systems using multilayer soft lithography and injection molding processes

상세보기
Lab Chip Glynn 14 2844 2014 10.1039/C4LC00264D Rapid, low-cost and instrument-free CD4+ cell counting for HIV diagnostics in resource-poor settings

상세보기
Lab Chip Begolo 14 4616 2014 10.1039/C4LC00910J The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications

상세보기
Lab Chip Park 18 1215 2018 10.1039/C7LC01128H Finger-actuated microfluidic device for the blood cross-matching test

상세보기
Anal. Chem. Park 91 11636 2019 10.1021/acs.analchem.9b02129 Finger-actuated microfluidic display for smart blood typing

상세보기
Lab Chip Park 19 2973 2019 10.1039/C9LC00422J Integrated microfluidic pumps and valves operated by finger actuation

상세보기
Micromachines Park 10 174 2019 10.3390/mi10030174 Finger-actuated microfluidic concentration gradient generator compatible with a microplate

상세보기
Infect. Immun. Dahan 72 5452 2004 10.1128/IAI.72.9.5452-5459.2004 Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes

상세보기
AIMS Microbiol. Trunk 4 140 2018 10.3934/microbiol.2018.1.140 Bacterial autoaggregation

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

원문 URL 링크

DOI : 10.1016/j.snb.2020.128346
Elsevier : 저널 > 논문

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

저작권 관리 안내

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증