[논문]High-performance portable graphene field-effect transistor device for detecting Gram-positive and -negative bacteria

Kim, Kyung Ho; Park, Seon Joo; Park, Chul Soon; Seo, Sung Eun; Lee, Jiyeon; Kim, Jinyeong; Lee, Seung Hwan; Lee, Soohyun; Kim, Jun-Seob; Ryu, Choong-Min; Yong, Dongeun; Yoon, Hyeonseok; Song, Hyun Seok; Lee, Sang Hun; Kwon, Oh Seok

doi:10.1016/j.bios.2020.112514

[해외논문] High-performance portable graphene field-effect transistor device for detecting Gram-positive and -negative bacteria

Biosensors & bioelectronics, v.167, 2020년, pp.112514 -

Kim, Kyung Ho (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Park, Seon Joo (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Park, Chul Soon (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Seo, Sung Eun (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Lee, Jiyeon (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Kim, Jinyeong (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Lee, Seung Hwan (Department of Bionano Engineering, Hanyang University) , Lee, Soohyun (Infectious Disease Research Center, Korea Research Institute of Bio) , Kim, Jun-Seob , Ryu, Choong-Min , Yong, Dongeun , Yoon, Hyeonseok , Song, Hyun Seok , Lee, Sang Hun , Kwon, Oh Seok

Abstract ▼ AI-Helper

Abstract Current techniques for Gram-typing and for diagnosing a pathogen at the early infection stage rely on Gram stains, cultures, Enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and gene microarrays, which are labor-intensive and time-consuming approaches. In addition, a delayed or imprecise diagnosis of clinical pathogenic bacteria leads to a life-threatening emergency or overuse of antibiotics and a high-rate occurrence of antimicrobial-resistance microbes. Herein, we report high-performance antibiotics (as bioprobes) conjugated graphene micropattern field-effect transistors (ABX-GMFETs) to facilitate on-site Gram-typing and help in the detection of the presence or absence of Gram-negative and -positive bacteria in the samples. The ABX-GMFET platform, which consists of recognition probes and GM transistors conjugated with novel interfacing chemical compounds, was integrated into the microfluidics to minimize the required human intervention and facilitate automation. The mechanism of binding of ABX-GMFET was based on a charge or chemical moiety interaction between the bioprobes and target bacteria. Subsequently, ABX-GMFETs exhibited unprecedented high sensitivity with a limit of detection (LOD) of 100 CFU/mL (1–9 CFU/mL), real-time target specificity. Highlights Serious side-effects in the excess of antibiotics have been issued. Nanotechnology to discriminate gram-positive and -negative bacteria are needed. On-site high-performance bacteria detection by graphene electronics were demonstrated. Novel interfacial chemicals for immobilizing bioreceptors on the graphene were synthesized. Graphene portable devices at the on-site were designed and applied.

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참고문헌 (52)

Chem. Rev. Allen 110 132 2010 10.1021/cr900070d

상세보기
ACS Nano An 7 10563 2013 10.1021/nn402702w

상세보기
Mater. Today Avouris 15 86 2012 10.1016/S1369-7021(12)70044-5

상세보기
Solid State Commun. Bolotin 146 351 2008 10.1016/j.ssc.2008.02.024

상세보기
Nanoscale Chang 5 3620 2013 10.1039/c3nr00141e

상세보기
ACS Appl. Mater. Interfaces Chen 6 3805 2014 10.1021/am500364f

상세보기
Pept. Res. Cuervo 1 81 1988
J. Dev. Econ. Currie 110 39 2014 10.1016/j.jdeveco.2014.05.006

상세보기
Appl. Environ. Microbiol. Dickson 55 832 1989 10.1128/AEM.55.4.832-836.1989

상세보기
Nat. Nanotechnol. Frank 5 487 2010 10.1038/nnano.2010.89

상세보기
Chem. Chem. Phys. Fujimoto 15 8983 2013 10.1039/c3cp50755f

상세보기
Biosens. Bioelectron. Garcia-Aljaro 25 2309 2010 10.1016/j.bios.2010.03.021

상세보기
Front. Bioeng. Biotechnol. Ghosh 6 1 2018 10.3389/fbioe.2018.00029

상세보기
Front. Microbiol. Goncalves 7 1 2016 10.3389/fmicb.2016.00013

상세보기
Biomaterials Hu 187 47 2018 10.1016/j.biomaterials.2018.09.019

상세보기
ACS Appl. Mater. Interfaces Im 9 10768 2017 10.1021/acsami.7b01163

상세보기
AAPS PharmSciTech Johnson 7 E33 2006 10.1208/pt070105

상세보기
Proc. Natl. Acad. Sci. Unit. States Am. Kennedy 105 1327 2008 10.1073/pnas.0710217105

상세보기
Sci. Rep. Kiraly 6 1 2016 10.1038/srep35455

상세보기
Adv. Mater. Kwon 25 4177 2013 10.1002/adma.201301523

상세보기
Nano Lett. Kwon 15 6559 2015 10.1021/acs.nanolett.5b02286

상세보기
Chem. Rev. Kwon 1 36 2019 10.1021/acs.chemrev.8b00159

상세보기
ACS Nano Lee 5 4373 2011 10.1021/nn103056s

상세보기
Int. J. Food Microbiol. Li 53 185 1999 10.1016/S0168-1605(99)00159-2

상세보기
Angew. Chem. Int. Ed. Li 55 8049 2016 10.1002/anie.201600205

상세보기
Nat. Commun. Liu 5 1 2014
Am. J. Obstet. Gynecol. Moscicki 157 65 1987 10.1016/S0002-9378(87)80347-2

상세보기
Adv. Mater. Technol. Moudgil 5 1 2020 10.1002/admt.201900615

상세보기
Anal. Chem. Oh 91 12181 2019 10.1021/acs.analchem.9b01068

상세보기
Exp. Ther. Med. Ouyang 9 1857 2015 10.3892/etm.2015.2315

상세보기
Nat. Nanotechnol. Pampaloni 13 755 2018 10.1038/s41565-018-0163-6

상세보기
Nano Lett. Park 12 5082 2012 10.1021/nl301714x

상세보기
ACS Nano Park 11 5950 2017 10.1021/acsnano.7b01722

상세보기
ACS Nano Ping 10 8700 2016 10.1021/acsnano.6b04110

상세보기
J. Nanosci. Nanotechnol. Pourasl 17 601 2017 10.1166/jnn.2017.12537

상세보기
Am. J. Obstet. Gynecol. Romero 159 114 1988 10.1016/0002-9378(88)90503-0

상세보기
Antimicrob. Agents Chemother. Rybak 42 721 1998 10.1128/AAC.42.3.721

상세보기
Nat. Commun. Seo 8 14217 2017 10.1038/ncomms14217

상세보기
Biochim. Biophys. Acta Biomembr. Shai 142 55 1999 10.1016/S0005-2736(99)00200-X

상세보기
Biosens. Bioelectron. Singh 136 23 2019 10.1016/j.bios.2019.04.029

상세보기
Nature Smith 561 189 2018 10.1038/s41586-018-0483-6

상세보기
Nano Lett. Song 13 172 2013 10.1021/nl3038147

상세보기
Curr. Microbiol. Soni 56 93 2008 10.1007/s00284-007-9046-z

상세보기
Int. J. Pharm. Takacsnovak 89 261 1993 10.1016/0378-5173(93)90252-B

상세보기
Biosens. Bioelectron. Thakur 110 16 2018 10.1016/j.bios.2018.03.014

상세보기
Nat. Immunol. Ugolini 19 386 2018 10.1038/s41590-018-0068-4

상세보기
Biosens. Bioelectron. Vaisocherova-Lisalova 80 84 2016 10.1016/j.bios.2016.01.040

상세보기
Sci. Rep. Wakshlak 5 1 2015 10.1038/srep09555

상세보기
Science Walsh 261 308 1993 10.1126/science.8392747

상세보기
Sensor. Actuator. B Chem. Wang 270 72 2018 10.1016/j.snb.2018.04.162

상세보기
Adv. Healthc. Mater. Wu 6 1 2017
Zasloff 389 1971

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[해외논문] High-performance portable graphene field-effect transistor device for detecting Gram-positive and -negative bacteria

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[해외논문] High-performance portable graphene field-effect transistor device for detecting Gram-positive and -negative bacteria

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