[논문]Visualization of unstained homo/heterogeneous DNA nanostructures by low-voltage scanning transmission electron microscopy

Gang, Geun Won; Shin, Jihoon; Kim, Young Heon; Ha, Tai Hwan; Ogawa, Takashi

doi:10.1038/s41598-020-61751-3

[해외논문] Visualization of unstained homo/heterogeneous DNA nanostructures by low-voltage scanning transmission electron microscopy 원문보기

Scientific reports, v.10, 2020년, pp.4868 -

Gang, Geun Won (Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), Daejeon, 34113 Republic of Korea) , Shin, Jihoon (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea) , Kim, Young Heon (Division of Industrial Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon, 34113 Republic of Korea) , Ha, Tai Hwan (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea) , Ogawa, Takashi (Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), Daejeon, 34113 Republic of Korea)

Abstract ▼ AI-Helper

Three-dimensional (3D) homo/heterogeneous DNA nanostructures were studied with low-voltage scanning transmission electron microscopy (LV-STEM). Four types of 3D DNA nanostructures were designed and fabricated by the origami method including newly proposed protocols. The low-energy electron probe and optimized dark-field STEM detector enabled individual unstained DNA nanostructures to be clearly imaged by the single acquisition without the averaging process. For the vertically stacked double structures, assembled through modified single-stranded domains, and the structures containing a square opening (i.e., a hole) in the center, the LV-STEM successfully reveals the vertical information of these 3D structures as the contrast differences compared to the reference. For the heterogeneous structures, the LV-STEM visualized both regions of the functionalized gold nanoparticles and the DNA base structure with distinct contrasts. This study introduces a straightforward method to fabricate stackable DNA nanostructures or nanoparticles by replacing a relatively small number of incumbent DNA strands, which could realize the simple and sophisticated fabrication of higher-order 3D DNA homo/hetero nanostructures. Together with these design techniques of DNA nanostructures, this study has demonstrated that the LV-STEM is the swift and simple method for visualizing the 3D DNA nanostructures and certifying the fabricated products as the specified design, which is applicable to various research fields on soft materials including DNA nanotechnology.

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