[논문]Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation

Zheng, Hong Xing; Chen, De Jing; Zu, Yue Xin; Wang, En Zhu; Qi, Shan Shan

doi:10.3390/ijms21155303

Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation 원문보기

International journal of molecular sciences, v.21 no.15, 2020년, pp.5303 -

Zheng, Hong Xing (College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China) , Chen, De Jing (cdjslg@126.com (D.J.C.)) , Zu, Yue Xin (jjlin1306@163.com (Y.X.Z.)) , Wang, En Zhu (a371505153@163.com (E.Z.W.)) , Qi, Shan Shan (College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China)

Abstract ▼ AI-Helper

Chondroitin sulfate (CS) has antioxidative, anti-inflammatory, anti-osteoarthritic and hypoglycemic effects. However, whether it has antidiabetic osteoporosis effects has not been reported. Therefore, in this study, we established a STZ-induced diabetic rat model; CS (500 mg kg−1 d−1) was orally administrated for eight weeks to study its preventive effects on diabetic osteoporosis. The results showed that eight weeks of CS treatment improved the symptoms of diabetes; the CS-treated group has increased body weight, decreased water or food intake, decreased blood glucose, increased bone-mineral density, repaired bone morphology and decreased femoral osteoclasts and tibia adipocytes numbers. After CS treatment, bone histomorphometric parameters returned to normal, the levels of serum inflammatory cytokines (IL-1β, IL-6 and TNF-α) decreased significantly, serum SOD, GPX and CAT activities increased and MDA level increased. In the CS-treated group, the levels of serum ALP, CTX-1, TRACP 5b, osteocalcin and RANKL decreased and the serum RUNX 2 and OPG levels increased. Bone immunohistochemistry results showed that CS can effectively increase the expression of OPG and RUNX2 and reduce the expression of RANKL in diabetic rats. All of these indicate that CS could prevent STZ induced diabetic osteoporosis—mainly through decreasing blood glucose, antioxidative stress, anti-inflammation and regulation of OPG/RANKL expression. CS can therefore effectively prevent bone loss caused by diabetes.

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참고문헌 (50)

1. Sumida Y. Shima T. Mitsumoto Y. Katayama T. Umemura A. Yamaguchi K. Itoh Y. Yoneda M. Okanoue T. Epidemiology, pathogenesis, and diagnostic strategy of diabetic liver disease in Japan Int. J. Mol. Sci. 2020 21 4337 10.3390/ijms21124337 32570776

상세보기
2. Montagnani A. Gonnelli S. Alessandri M. Nuti R. Osteoporosis and risk of fracture in patients with diabetes: An update Aging Clin. Exp. Res. 2011 23 84 90 10.1007/BF03351073 21743287

상세보기
3. Qi S.S. He J. Han H. Zheng H.X. Hu Q.Y. Jiang H. Li X.S. Anthocyanin-Rich extract from black rice (Oryza sativa L. Japonica) ameliorates diabetic osteoporosis in rats Food Funct. 2019 10 5350 5360 10.1039/C9FO00681H 31393485

상세보기
4. Qi S.S. He J. Zheng H.X. Lan S.Q. Icariin prevents diabetes-induced bone loss in rats by reducing blood glucose and suppressing bone turnover Molecules 2019 24 1871 10.3390/molecules24101871 31096652

상세보기
5. Napoli N. Chandran M. Pierroz D. Abrahamsen B. Schwartz A.V. Ferrari S.L. Mechanisms of diabetes mellitus-induced bone fragility Nat. Rev. Endocrinol. 2017 13 208 219 10.1038/nrendo.2016.153 27658727

상세보기
6. Rathinavelu S. Guidry-Elizondo C. Banu J. Molecular modulation of osteoblasts and osteoclasts in type 2 diabetes J. Diabetes Res. 2018 2018 6354787 10.1155/2018/6354787 30525054

상세보기
7. Kalyanaraman H. Schwaerzer G. Ramdani G. Castillo F. Scott B.T. Dillmann W. Sah R.L. Casteel D.E. Pilz R.B. Protein kinase G activation reverses oxidative stress and restores osteoblast function and bone formation in male mice with type 1 diabetes Diabetes 2018 67 607 623 10.2337/db17-0965 29301852

상세보기
8. Guo C.J. Xie J.J. Hong R.H. Pan H.S. Zhang F.G. Liang Y.M. Puerarin alleviates streptozotocin (STZ)-induced osteoporosis in rats through suppressing inflammation and apoptosis via HDAC1/HDAC3 signaling Biomed. Pharmacother. 2019 115 108570 10.1016/j.biopha.2019.01.031 31054509

상세보기
9. Asadipooya K. Uy E.M. Advanced glycation end products (AGEs), receptor for AGEs, diabetes, and bone: Review of the literature J. Endocr. Soc. 2019 3 1799 1818 10.1210/js.2019-00160 31528827

상세보기
10. Kanazawa I. Interaction between bone and glucose metabolism Endocr. J. 2017 64 1043 1053 10.1507/endocrj.EJ17-0323 28966224

상세보기
11. Loi F. Cordova L.A. Pajarinen J. Lin T.H. Yao Z. Goodman S.B. Inflammation, fracture and bone repair Bone 2016 86 119 130 10.1016/j.bone.2016.02.020 26946132

상세보기
12. Rao S.S. Hu Y. Xie P.L. Omentin-1 prevents inflammation-induced osteoporosis by down-regulating the pro-inflammatory cytokines Bone Res. 2018 6 9 21 10.1038/s41413-018-0012-0 29619269

상세보기
13. Ginaldi L. De Martinis M. Osteoimmunology and beyond Curr. Med. Chem. 2016 23 3754 3774 10.2174/0929867323666160907162546 27604089

상세보기
14. De Martinis M. Sirufo M.M. Nocelli C. Fontanella L. Ginaldi L. Hyperhomocysteinemia is associated with inflammation, bone resorption, vitamin B12 and folate deficiency and MTHFR C677T polymorphism in postmenopausal women with decreased bone mineral density Int. J. Environ. Res. Public Health 2020 17 4260 10.3390/ijerph17124260 32549258

상세보기
15. Katayama Y. Naitoh M. Kubota H. Yamawaki S. Aya R. Ishiko T. Morimoto N. Chondroitin sulfate promotes the proliferation of keloid fibroblasts through activation of the integrin and protein kinase B pathways Int. J. Mol. Sci. 2020 21 1955 10.3390/ijms21061955 32182995

상세보기
16. Rani A. Patel S. Goyal A. Chondroitin sulfate (CS) lyases: Structure, function and application in therapeutics Curr. Protein Pept. Sci. 2018 19 22 33 10.2174/1389203718666170102112805 28049392

상세보기
17. Ju C. Hou L. Sun F. Zhang Z. Gao H. Wang L. Wang D. Lv Y. Zhao X. Anti-Oxidation and anti-apoptotic effects of chondroitin sulfate on 6-hydroxydopamine-induced injury through the up-regulation of Nrf2 and inhibition of mitochondria-mediated pathway Neurochem. Res. 2015 40 1509 1519 10.1007/s11064-015-1628-8 26033682

상세보기
18. Nagano F. Mizuno T. Mizumoto S. Yoshioka K. Takahashi K. Tsuboi N. Maruyama S. Yamada S. Nagamatsu T. Chondroitin sulfate protects vascular endothelial cells from toxicities of extracellular histones Eur. J. Pharmacol. 2018 826 48 55 10.1016/j.ejphar.2018.02.043 29501579

상세보기
19. Motoab M. Takamizawac N. Shibuyad T. Nakamurab A. Katsurayaa K. Katsuyuki K. Anti-Diabetic effects of chondroitin sulfate on normal and type 2 diabetic mice J. Funct. Foods 2018 40 336 340

상세보기
20. Gomes C.L.R. Leao C.L. Venturotti C. Barreira A.L. Guimaraes G. Fonseca R.S. Mourao P.A.S. Delgado A.G. Takiya C.M. Leite M. The protective role of fucosylated chondroitin sulfate, a distinct glycosaminoglycan, in a murine model of streptozotocin-induced diabetic nephropathy PLoS ONE 2014 9 e106929 10.1371/journal.pone.0106929 25192337

상세보기
21. Hu S. Chang Y. He M. Wang J. Wang Y. Xue C. Fucosylated chondroitin sulfate from sea cucumber improves insulin sensitivity via activation of PI3K/PKB pathway J. Food Sci. 2014 79 1424 1429 10.1111/1750-3841.12465 25041539

상세보기
22. Carnovali M. Luzi L. Banfi G. Mariotti M. Chronic hyperglycemia affects bone metabolism in adult zebrafish scale model Endocrine 2016 54 808 817 10.1007/s12020-016-1106-3 27696252

상세보기
23. Jiang N. Xia W. Assessment of bone quality in patients with diabetes mellitus Osteoporos. Int. 2018 29 1721 1736 10.1007/s00198-018-4532-7 29736760

상세보기
24. Yamamoto M. Sugimoto T. Advanced glycation end products, diabetes, and bone strength Curr. Osteoporos. Rep. 2016 14 320 326 10.1007/s11914-016-0332-1 27704396

상세보기
25. De Martinis M. Ginaldi L. Sirufo M.M. Pioggia G. Calapai G. Gangemi S. Mannucci C. Alarmins in osteoporosis, RAGE, IL-1, and IL-33 pathways: A literature review Medicina (Kaunas) 2020 56 138 10.3390/medicina56030138 32204562

상세보기
26. Karstoft K. Pedersen B.K. Exercise and type 2 diabetes: Focus on metabolism and inflammation Immunol. Cell Biol. 2016 94 146 150 10.1038/icb.2015.101 26568029

상세보기
27. Marahleh A. Kitaura H. Ohori F. Fujita K. Kawabata T. Kozawa O. Otsuka T. TNF-α directly enhances osteocyte RANKL expression and promotes osteoclast formation Front Immunol. 2019 10 2925 10.3389/fimmu.2019.02925 31921183

상세보기
28. Wang T. He C. TNF-α and IL-6: The link between immune and bone system Curr. Drug Targets 2020 21 213 227 10.2174/1389450120666190821161259 31433756

상세보기
29. Shiratori T. Kyumoto-Nakamura Y. Kukita A. IL-1β induces pathologically activated osteoclasts bearing extremely high levels of resorbing activity: A possible pathological subpopulation of osteoclasts, accompanied by suppressed expression of kindlin-3 and talin-1 J. Immunol. 2018 200 218 228 10.4049/jimmunol.1602035 29141864

상세보기
30. Bacevic M. Brkovic B. Albert A. Rompen E. Radermecker R.P. Lambert F. Does oxidative stress play a role in altered characteristics of diabetic bone? A systematic review Calcif. Tissue Int. 2017 101 553 563 10.1007/s00223-017-0327-7 29063963

상세보기
31. Cheng Y.Z. Yang S.L. Wang J.Y. Irbesartan attenuates advanced glycation end products-mediated damage in diabetes-associated osteoporosis through the AGEs/RAGE pathway Life Sci. 2018 205 184 192 10.1016/j.lfs.2018.04.042 29702126

상세보기
32. Starup-Linde J. Eriksen S.A. Lykkeboe S. Handberg A. Vestergaard P. Biochemical markers of bone turnover in diabetes patients-A meta-analysis, and a methodological study on the effects of glucose on bone markers Osteoporos. Int. 2014 25 1697 1708 10.1007/s00198-014-2676-7 24676844

상세보기
33. Zheng H. Qi S.S. Chen C. Salidroside improves bone histomorphology and prevents bone loss in ovariectomized diabetic rats by upregulating the OPG/RANKL ratio Molecules 2018 23 2398 10.3390/molecules23092398 30235836

상세보기
34. Kovacs B. Vajda E. Nagy E.E. Regulatory effects and interactions of the Wnt and OPG-RANKL-RANK signaling at the bone-cartilage interface in osteoarthritis Int. J. Mol. Sci. 2019 20 4653 10.3390/ijms20184653

상세보기
35. Chen X. Wang Z. Duan N. Zhu G. Schwarz E.M. Xie C. Osteoblast-Osteoclast interactions Connect Tissue Res. 2018 59 99 107 10.1080/03008207.2017.1290085 28324674

상세보기
36. Conte A. Ghiraldini B. Casarin R.C. Impact of type 2 diabetes on the gene expression of bone-related factors at sites receiving dental implants Int. J. Oral Maxillofac. Surg. 2015 44 1302 1308 10.1016/j.ijom.2015.06.001 26112994

상세보기
37. Qi S.S. He J. Zheng H. Chen C. Jiang H. Lan S. Zinc supplementation increased bone mineral density, improves bone histomorphology, and prevents bone loss in diabetic rat Biol. Trace Elem. Res. 2020 194 493 501 10.1007/s12011-019-01810-7 31363990

상세보기
38. Yin Q. Wang J. Fu Q. Gu S. Rui Y. CircRUNX2 through has-miR-203 regulates RUNX2 to prevent osteoporosis J. Cell Mol. Med. 2018 22 6112 6121 10.1111/jcmm.13888 30324718

상세보기
39. Zhang X. Chen K. Wei B. Liu X. Lei Z. Bai X. Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway Chem. Biol. Interact. 2016 256 188 197 10.1016/j.cbi.2016.07.003 27387537

상세보기
40. Adil M. Khan R.A. Kalam A. Venkata S.K. Kandhare A.D. Ghosh P. Sharma M. Effect of anti-diabetic drugs on bone metabolism: Evidence from preclinical and clinical studies Pharmacol. Rep. 2017 69 1328 1340 10.1016/j.pharep.2017.05.008 29132091

상세보기
41. Wang C. Meng H. Wang X. Zhao C. Peng J. Wang Y. Differentiation of bone marrow mesenchymal stem cells in osteoblasts and adipocytes and its role in treatment of osteoporosis Med. Sci. Monit. 2016 22 226 233 10.12659/MSM.897044 26795027

상세보기
42. Rivoira M. Rodriguez V. Picotto G. Battaglino R. Naringin prevents bone loss in a rat model of type 1 Diabetes mellitus Arch. Biochem. Biophys. 2018 637 56 63 10.1016/j.abb.2017.12.001 29208404

상세보기
43. Piccinin M.A. Khan Z.A. Pathophysiological role of enhanced bone marrow adipogenesis in diabetic complications Adipocytes 2014 3 263 272 10.4161/adip.32215 26317050

상세보기
44. Lin J.T. Ji B.Y. Zhang Y.C. Role of metformin on osteoblast differentiation in type 2 diabetes Biomed. Res. Int. 2019 2019 9203934 31886264

상세보기
45. Bahrambeigi S. Yousefi B. Rahimi M. Shafiei-Irannejad V. Metformin, an old antidiabetic drug with new potentials in bone disorders Biomed. Pharmacother. 2019 109 1593 1601 10.1016/j.biopha.2018.11.032 30551413

상세보기
46. Zheng L. Shen X. Ye J. Xie Y. Yan S. Metformin alleviates hyperglycemia-induced apoptosis and differentiation suppression in osteoblasts through inhibiting the TLR4 signaling pathway Life Sci. 2019 216 29 38 10.1016/j.lfs.2018.11.008 30414431

상세보기
47. Rani A. Baruah R. Goyal A. Physicochemical, antioxidant and biocompatible properties of chondroitin sulphate isolated from chicken keel bone for potential biomedical applications Carbohydr. Polym. 2017 159 11 19 10.1016/j.carbpol.2016.12.015 28038739

상세보기
48. Zou Z. Wei M. Fang J. Dai W. Sun T. Liu Q. Gong G. Liu Y. Song S. Ma F. Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities Int. J. Biol. Macromol. 2020 152 1047 1055 10.1016/j.ijbiomac.2019.10.192 31751707

상세보기
49. De Martinis M. Sirufo M.M. Ginaldi L. Osteoporosis: Current and emerging therapies targeted to immunological checkpoints Curr. Med. Chem. 2019 10.2174/0929867326666190730113123

상세보기
50. Revell P.A. Histomorphometry of bone J. Clin. Pathol. 1983 36 1323 1331 10.1136/jcp.36.12.1323 6361070

상세보기

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Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation 원문보기

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