[논문]Serial optical coherence microscopy for label-free volumetric histopathology

Min, Eunjung; Ban, Sungbea; Lee, Junwon; Vavilin, Andrey; Baek, Songyee; Jung, Sunwoo; Ahn, Yujin; Park, Kibeom; Shin, Sungwon; Han, SoHyun; Cho, Hyungjoon; Lee-Kwon, Whaseon; Kim, Jeehyun; Lee, C. Justin; Jung, Woonggyu

doi:10.1038/s41598-020-63460-3

[해외논문] Serial optical coherence microscopy for label-free volumetric histopathology 원문보기

Scientific reports, v.10, 2020년, pp.6711 -

Min, Eunjung (Max Planck Institute for Biological Cybernetics, 72076 Tü) , Ban, Sungbea (bingen, Germany) , Lee, Junwon (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Vavilin, Andrey (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Baek, Songyee (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Jung, Sunwoo (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Ahn, Yujin (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Park, Kibeom (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Shin, Sungwon (Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919 Republic of Korea) , Han, SoHyun (Dep) , Cho, Hyungjoon , Lee-Kwon, Whaseon , Kim, Jeehyun , Lee, C. Justin , Jung, Woonggyu

Abstract ▼ AI-Helper

The observation of histopathology using optical microscope is an essential procedure for examination of tissue biopsies or surgically excised specimens in biological and clinical laboratories. However, slide-based microscopic pathology is not suitable for visualizing the large-scale tissue and native 3D organ structure due to its sampling limitation and shallow imaging depth. Here, we demonstrate serial optical coherence microscopy (SOCM) technique that offers label-free, high-throughput, and large-volume imaging of ex vivo mouse organs. A 3D histopathology of whole mouse brain and kidney including blood vessel structure is reconstructed by deep tissue optical imaging in serial sectioning techniques. Our results demonstrate that SOCM has unique advantages as it can visualize both native 3D structures and quantitative regional volume without introduction of any contrast agents.

참고문헌 (36)

1. Gillett C Springall R Barnes D Hanby A Multiple tissue core arrays in histopathology research: a validation study J. Pathol. 2000 192 549 553 10.1002/1096-9896(2000)9999:9999<::AID-PATH721>3.0.CO;2-0 11113875

상세보기
2. Roberts N Toward routine use of 3D histopathology as a research tool Am. J. Pathol. 2012 180 1835 1842 10.1016/j.ajpath.2012.01.033 22490922

상세보기
3. Magee, D. et al . Histopathology in 3D: From three-dimensional reconstruction to multi-stain and multi-modal analysis. J. Pathol. Inform . 6 (2015).
4. Pezacki JP Chemical contrast for imaging living systems: molecular vibrations drive CARS microscopy Nat. Chem. Biol. 2011 7 137 10.1038/nchembio.525 21321552

상세보기
5. Farrar MJ Wise FW Fetcho JR Schaffer CB In vivo imaging of myelin in the vertebrate central nervous system using third harmonic generation microscopy Biophys. J. 2011 100 1362 1371 10.1016/j.bpj.2011.01.031 21354410

상세보기
6. Schain AJ Hill RA Grutzendler J Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy Nat. Med. 2014 20 443 10.1038/nm.3495 24681598

상세보기
7. Sridharan S Macias V Tangella K Kajdacsy-Balla A Popescu G Prediction of prostate cancer recurrence using quantitative phase imaging Sci. Rep. 2015 5 9976 10.1038/srep09976 25975368

상세보기
8. Lu, F.-K. et al . Label-free neurosurgical pathology with stimulated Raman imaging. Cancer Res., canres . 0270.2016 (2016).
9. Tu H Stain-free histopathology by programmable supercontinuum pulses Nat. Photonics 2016 10 534 10.1038/nphoton.2016.94 27668009

상세보기
10. Wong TT Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy Nat. Commun. 2017 8 1386 10.1038/s41467-017-01649-3 29123109

상세보기
11. Li A Micro-optical sectioning tomography to obtain a high-resolution atlas of the mouse brain Science 2010 330 1404 1408 10.1126/science.1191776 21051596

상세보기
12. Gong H Continuously tracing brain-wide long-distance axonal projections in mice at a one-micron voxel resolution NeuroImage 2013 74 87 98 10.1016/j.neuroimage.2013.02.005 23416252

상세보기
13. Ragan T Serial two-photon tomography for automated ex vivo mouse brain imaging Nat. Methods 2012 9 255 10.1038/nmeth.1854 22245809

상세보기
14. Dodt H-U Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain Nat. Methods 2007 4 331 10.1038/nmeth1036 17384643

상세보기
15. Susaki EA Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis Cell 2014 157 726 739 10.1016/j.cell.2014.03.042 24746791

상세보기
16. Tainaka K Whole-body imaging with single-cell resolution by tissue decolorization Cell 2014 159 911 924 10.1016/j.cell.2014.10.034 25417165

상세보기
17. Renier N iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging Cell 2014 159 896 910 10.1016/j.cell.2014.10.010 25417164

상세보기
18. Belle M A simple method for 3D analysis of immunolabeled axonal tracts in a transparent nervous system Cell reports 2014 9 1191 1201 10.1016/j.celrep.2014.10.037 25456121

상세보기
19. Tomer R Ye L Hsueh B Deisseroth K Advanced CLARITY for rapid and high-resolution imaging of intact tissues Nat. Protoc. 2014 9 1682 10.1038/nprot.2014.123 24945384

상세보기
20. Richardson DS Lichtman JW Clarifying tissue clearing Cell 2015 162 246 257 10.1016/j.cell.2015.06.067 26186186

상세보기
21. Azaripour A A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue Prog. Histochem. Cytochem. 2016 51 9 23 10.1016/j.proghi.2016.04.001 27142295

상세보기
22. Huang D Optical coherence tomography Science 1991 254 1178 1181 10.1126/science.1957169 1957169

상세보기
23. Merkle CW Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo Opt. Lett. 2018 43 198 201 10.1364/OL.43.000198 29328237

상세보기
24. Wang H Polarization sensitive optical coherence microscopy for brain imaging Opt. Lett. 2016 41 2213 2216 10.1364/OL.41.002213 27176965

상세보기
25. Min E Wide-field optical coherence microscopy of the mouse brain slice Opt. Lett. 2015 40 4420 4423 10.1364/OL.40.004420 26421546

상세보기
26. Srinivasan VJ Radhakrishnan H Jiang JY Barry S Cable AE Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast Opt. Express 2012 20 2220 2239 10.1364/OE.20.002220 22330462

상세보기
27. Jafri MS Optical coherence tomography in the diagnosis and treatment of neurological disorders JBO 2005 10 051603 10.1117/1.2116967

상세보기
28. Hariri LP Mino-Kenudson M Mark EJ Suter MJ In vivo optical coherence tomography: the role of the pathologist Arch. Pathol. Lab. Med. 2012 136 1492 1501 10.5858/arpa.2012-0252-SA 23194041

상세보기
29. Assayag O Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography NeuroImage: clinical 2013 2 549 557 10.1016/j.nicl.2013.04.005 24179806

상세보기
30. Iyer JS Micro-optical coherence tomography of the mammalian cochlea Sci. Rep. 2016 6 33288 10.1038/srep33288 27633610

상세보기
31. Leahy C Radhakrishnan H Srinivasan VJ Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast Biomed. Opt. Express 2013 4 1978 1990 10.1364/BOE.4.001978 24156058

상세보기
32. Ucero AC Unilateral ureteral obstruction: beyond obstruction Int. Urol. Nephrol. 2014 46 765 776 10.1007/s11255-013-0520-1 24072452

상세보기
33. Evans RW The quality of life of patients with end-stage renal disease New Engl. J. Med. 1985 312 553 559 10.1056/NEJM198502283120905 3918267

상세보기
34. Hiorns MP Imaging of the urinary tract: the role of CT and MRI Pediatr. Nephrol. 2011 26 59 68 10.1007/s00467-010-1645-4 20924611

상세보기
35. Garcia-Sanz A Rodriguez-Barbero A Bentley MD Ritman EL Romero JC Three-dimensional microcomputed tomography of renal vasculature in rats Hypertension 1998 31 440 444 10.1161/01.HYP.31.1.440 9453342

상세보기
36. Eddy AA Molecular insights into renal interstitial fibrosis J. Am. Soc. Nephrol. 1996 7 2495 2508 8989727

상세보기

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