[논문]Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

Lee, Wang Sik; Kang, Taejoon; Kwak, Kyung Jin; Park, Kyoungsook; Yi, So Yeon; Lee, Ui Jin; Shin, Yong Beom; Jeong, Jinyoung

doi:10.1186/s40580-020-00223-w

[해외논문] Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase 원문보기

Nano convergence, v.7, 2020년, pp.13 -

Lee, Wang Sik (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Kang, Taejoon (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Kwak, Kyung Jin (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Park, Kyoungsook (Dept. of General Education, Daejeon Health Institute of Technology, 21 Chungjeon-ro, Dong-gu, Daejeon, 34504 Republic of Korea) , Yi, So Yeon (BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Lee, Ui Jin (BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Shin, Yong Beom (Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113 Republic of Korea) , Jeong, Jinyoung (Env)

Abstract ▼ AI-Helper

This work reports on a rapid diagnostic platform for the detection of Plasmodium falciparum lactate dehydrogenase (PfLDH), a representative malaria biomarker, using a microfluidic microplate-based immunoassay. In this study, the microfluidic microplate made it possible to diagnose PfLDH with a small volume of sample (only 5 μL) and short time (< 90 min) compared to conventional immunoassays such as enzyme-linked immunosorbent assay (ELISA). Moreover, the diagnostic performance of PfLDH showed high sensitivity, specificity, and selectivity (i.e., 0.025 pg/μL in phosphate-buffered saline and 1 pg/μL in human serum). The microfluidic-based microplate sensing platform has the potential to adapt simple, rapid, and accurate diagnoses to the practical detection of malaria.

Keyword

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