[논문]Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles

Bok, Moonjeong; Zhao, Zhi-Jun; Hwang, Soon Hyoung; Kang, Hyeok-Joong; Jeon, Sohee; Ko, Jiwoo; Jeong, Jiwon; Song, Young Seok; Lim, Eunju; Jeong, Jun-Ho

doi:10.3390/pharmaceutics12100954

[해외논문] Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles 원문보기

Pharmaceutics, v.12 no.10, 2020년, pp.954 -

Bok, Moonjeong (Nano-Convergence Mechanical Systems Research Division, Korea Institute of Machinery and Materials, Daejeon 34103, Korea) , Zhao, Zhi-Jun (bokmj@kimm.re.kr (M.B.)) , Hwang, Soon Hyoung (zhaozhijun@kimm.re.kr (Z.-J.Z.)) , Kang, Hyeok-Joong (soon814@kimm.re.kr (S.H.H.)) , Jeon, Sohee (kanghj@kimm.re.kr (H.-J.K.)) , Ko, Jiwoo (sjeon@kimm.re.kr (S.J.)) , Jeong, Jiwon (jjiwoo@kimm.re.kr (J.K.)) , Song, Young Seok (Nano-Convergence Mechanical Systems Research Division, Korea Institute of Machinery and Materials, Daejeon 34103, Korea) , Lim, Eunju (bokmj@kimm.re.kr (M.B.)) , Jeong, Jun-Ho (zhaozhijun@kimm.re.kr (Z.-J.Z.))

Abstract ▼ AI-Helper

Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 μm) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform.

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