[논문]Normal-incidence type solution immersed silicon (SIS) biosensor for ultra-sensitive, label-free detection of cardiac troponin I

Kim, Dong Hyung; Chegal, Won; Cho, Yong Jai; Won O, Sang; Van Le, Long; Diware, Mangesh S.; Paek, Se-Hwan; Kim, Young Dong; Cho, Hyun Mo

doi:10.1016/j.bios.2020.112525

[해외논문] Normal-incidence type solution immersed silicon (SIS) biosensor for ultra-sensitive, label-free detection of cardiac troponin I

Biosensors & bioelectronics, v.168, 2020년, pp.112525 -

Kim, Dong Hyung (Division of Interdisciplinary Materials Measurement Institute, Korea Research Institute of Standards and Science) , Chegal, Won (Division of Advanced Instrumentation Institute, Korea Research Institute of Standards and Science) , Cho, Yong Jai (Division of Advanced Instrumentation Institute, Korea Research Institute of Standards and Science) , Won O, Sang (Division of Interdisciplinary Materials Measurement Institute, Korea Research Institute of Standards and Science) , Van Le, Long (Division of Interdisciplinary Materials Measurement Institute, Korea Research Institute of Standards and Science) , Diware, Mangesh S. (CeNSCMR and Institute of Applied Physics, Department of Physics and Astronomy, Seoul National University) , Paek, Se-Hwan (Department of Bio-Microsystem Technology, Korea University) , Kim, Young Dong (Department of Physics, Kyung Hee University South Korea<) , Cho, Hyun Mo

Abstract ▼ AI-Helper

Abstract Early diagnosis of acute myocardial infarction (AMI) significantly reduce the mortality rate and can be achieved via high-sensitive detection of AMI specific cardiac troponin I (cTnI) biomarker. Here, we present normal-incident type solution-immersed silicon (NI–SIS) ellipsometric biosensor, designed for ultra-high sensitive, high-throughput, label-free detection of the target protein. The NI–SIS sensors are equipped with a specially designed prism that maintains the angle of incidence close to the Brewster angle during operation, which significantly reduces SIS noise signals induced by the refractive index fluctuations of the surrounding medium, improves the signal-to-noise ratio, in-results lowers the detection limit. We applied NI–SIS biosensor for ultra-sensitive detection of cTnI biomarkers in human serum. The optimized sensor chip fabrication and detection operation procedures are proposed. The wide linear concentration ranges of fg/mL to ng/mL is achieved with the detection limit of 22.0 fg/mL of cTnI. The analytical correlation was assessed by linear regression analysis with the results of the Pathfast reference system. These impressive biosensing capabilities of NI–SIS technology have huge potentials for accurate detection of target species in different application areas, such as diagnosis, drug discovery, and food contaminations. Highlights We investigated ultra-sensitive, label-free detection techniques using normal-incidence type solution immersed silicon (NI–SIS) biosensor. The NI–SIS sensor was equipped with a specially designed prism that maintains the angle of incidence close to the Brewster angle during operation. The SiO2 layer thickness over the sensor chip was optimized from experiments and simulations to minimize local reflective index changes inducing noisy signals. We could measure the detection limit of 22.0 fg/mL for cardiac troponin I in human serum.

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