[논문]Remodeling of Adhesion Network within Cancer Spheroids via Cell-Polymer Interaction

Cho, Youngbin; Yu, Seung Jung; Kim, Jiwon; Ko, Ung Hyun; Park, Eun Young; Choung, Jin Seung; Choi, Goro; Kim, Daehyun; Lee, Eunjung; Im, Sung Gap; Shin, Jennifer H.

doi:10.1021/acsbiomaterials.0c00977

[해외논문] Remodeling of Adhesion Network within Cancer Spheroids via Cell-Polymer Interaction

ACS biomaterials science & engineering, v.6 no.10, 2020년, pp.5632 - 5644

Cho, Youngbin (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Republic of Korea) , Yu, Seung Jung (KAIST Institute for the NanoCentury, Department of Chemical and Biomolecular Engineering , KAIST , 291, Daehak-ro , Daejeon 34141 , Republic of Korea) , Kim, Jiwon (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Republic of Korea) , Ko, Ung Hyun (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Republic of Korea) , Park, Eun Young (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Republic of Korea) , Choung, Jin Seung (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Republic of Korea Republic o) , Choi, Goro , Kim, Daehyun , Lee, Eunjung , Im, Sung Gap , Shin, Jennifer H.

Abstract ▼ AI-Helper

3D spheroids are considered as the improved in vitro model to mimic the distinct arrangements of the cells in vivo. To date, low-attachment surfaces have been most widely used to induce the spontaneous aggregation of cells in suspension by simply tuning the relative strength of the cell-cell adhesion over cell-substrate adhesion. However, aggregating cancer cells into 3D clusters should mean more than just adjoining the cells in the physical proximity. The tumor cell functionality is strongly affected by the adhesion networks between cancer cells and extracellular matrix (ECM). Here, we performed an in-depth analysis of how the nonmetastatic breast cancer cells (MCF7) can be transformed to gain invasive phenotypes through compact aggregation into 3D spheroids on a functional polymer film surface, poly(2,4,6,8-tetravinyl-2,4,6,8-tetramethyl cyclotetrasiloxane) (pV4D4). By comparing the adhesion networks and invasion dynamics between 3D spheroids cultured on the pV4D4 surface with those cultured on conventional ultra-low-attachment (ULA) dishes, we report that only spheroids on the pV4D4 display active and sporadic cell-surface binding activities via dynamic protrusions, which correlates strongly with an increase in integrin β1. Moreover, localized laminin expression at the core of the pV4D4-cultured spheroids confirms the prominence of the intimate integrin-laminin interactions prompted by the exposure to pV4D4. This study suggests that structurally and functionally dissimilar 3D spheroids can be generated from the same type of cells on the surfaces of different physicochemical properties without any chemical treatment or genetic manipulation.[FIG OMISSION]

Keyword

참고문헌 (61)

Pampaloni, Francesco, Reynaud, Emmanuel G., Stelzer, Ernst H. K.. The third dimension bridges the gap between cell culture and live tissue. Nature reviews. Molecular cell biology, vol.8, no.10, 839-845.

상세보기
Edmondson, Rasheena, Broglie, Jessica Jenkins, Adcock, Audrey F., Yang, Liju. Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors. Assay and drug development technologies, vol.12, no.4, 207-218.

상세보기
Lin, Ruei‐Zhen, Chang, Hwan‐You. Recent advances in three‐dimensional multicellular spheroid culture for biomedical research. Biotechnology journal, vol.3, no.9, 1172-1184.

상세보기
Weiswald, Louis-Bastien, Bellet, Dominique, Dangles-Marie, Virginie. Spherical Cancer Models in Tumor Biology ¹. Neoplasia : an international journal for oncology research, vol.17, no.1, 1-15.

상세보기
Zhong, Xiaoling, Rescorla, Frederick J.. Cell surface adhesion molecules and adhesion-initiated signaling: Understanding of anoikis resistance mechanisms and therapeutic opportunities. Cellular signalling, vol.24, no.2, 393-401.

상세보기
Egeblad, Mikala, Nakasone, Elizabeth S., Werb, Zena. Tumors as Organs: Complex Tissues that Interface with the Entire Organism. Developmental cell, vol.18, no.6, 884-901.

상세보기
Wiseman, Bryony S., Werb, Zena. Stromal Effects on Mammary Gland Development and Breast Cancer. Science, vol.296, no.5570, 1046-1049.

상세보기
Rijal, G., Li, W.. 3D scaffolds in breast cancer research. Biomaterials, vol.81, 135-156.

상세보기
10.1002/(SICI)1096-9896(199705)182:1<36::AID-PATH802>3.0.CO;2-W

상세보기
Ioachim, E, Charchanti, A, Briasoulis, E, Karavasilis, V, Tsanou, H, Arvanitis, D.L, Agnantis, N.J, Pavlidis, N. Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression. European journal of cancer, vol.38, no.18, 2362-2370.

상세보기
White, Donald E., Kurpios, Natasza A., Zuo, Dongmei, Hassell, John A., Blaess, Sandra, Mueller, Ulrich, Muller, William J.. Targeted disruption of β1-integrin in a transgenic mouse model of human breast cancer reveals an essential role in mammary tumor induction. Cancer cell, vol.6, no.2, 159-170.

상세보기
Yao, Evelyn S., Zhang, Hui, Chen, Yunn-Yi, Lee, Brian, Chew, Karen, Moore, Dan, Park, Catherine. Increased β1 Integrin Is Associated with Decreased Survival in Invasive Breast Cancer. Cancer research : the official organ of the American Association for Cancer Research, Inc, vol.67, no.2, 659-664.

상세보기
Sethi, Tariq, Rintoul, Robert C., Moore, Sarah M., MacKinnon, Alison C., Salter, Donald, Choo, Chin, Chilvers, Edwin R., Dransfield, Ian, Donnelly, Seamas C., Strieter, Robert, Haslett, Christopher. Extracellular matrix proteins protect small cell lung cancer cells against apoptosis: A mechanism for small cell lung cancer growth and drug resistance in vivo. Nature medicine, vol.5, no.6, 662-668.

상세보기
Weaver, V.M., Petersen, O.W., Wang, F., Larabell, C.A., Briand, P., Damsky, C., Bissell, M.J.. Reversion of the Malignant Phenotype of Human Breast Cells in Three-Dimensional Culture and In Vivo by Integrin Blocking Antibodies. The Journal of cell biology, vol.137, no.1, 231-245.

상세보기
Wang, Fei, Weaver, Valerie M., Petersen, Ole W., Larabell, Carolyn A., Dedhar, Shoukat, Briand, Per, Lupu, Ruth, Bissell, Mina J.. Reciprocal interactions between β1-integrin and epidermal growth factor receptor in three-dimensional basement membrane breast cultures: A different perspective in epithelial biology. Proceedings of the National Academy of Sciences of the United States of America, vol.95, no.25, 14821-14826.

상세보기
Cancer. Res. Bissell M. J. 1757 59 1999

상세보기
Canel, Marta, Serrels, Alan, Frame, Margaret C., Brunton, Valerie G.. E-cadherin–integrin crosstalk in cancer invasion and metastasis. Journal of cell science, vol.126, no.2, 393-401.

상세보기
Kim, Jong Bin. Three-dimensional tissue culture models in cancer biology. Seminars in cancer biology, vol.15, no.5, 365-377.

상세보기
Cui, X., Hartanto, Y., Zhang, H.. Advances in multicellular spheroids formation. Journal of the Royal Society, Interface, vol.14, no.127, 20160877-20160877.

상세보기
Zhang, W., Li, C., Baguley, B.C., Zhou, F., Zhou, W., Shaw, J.P., Wang, Z., Wu, Z., Liu, J.. Optimization of the formation of embedded multicellular spheroids of MCF-7 cells: How to reliably produce a biomimetic 3D model. Analytical biochemistry, vol.515, 47-54.

상세보기
Li, Yunfeng, Kumacheva, Eugenia. Hydrogel microenvironments for cancer spheroid growth and drug screening. Science advances, vol.4, no.4, eaas8998-.

상세보기
Ivascu, Andrea, Kubbies, Manfred. Rapid Generation of Single-Tumor Spheroids for High-Throughput Cell Function and Toxicity Analysis. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening, vol.11, no.8, 922-932.

상세보기
10.7150/jca.2.458
Froehlich, Karolin, Haeger, Jan-Dirk, Heger, Julia, Pastuschek, Jana, Photini, Stella Mary, Yan, Lupp, Amelie, Pfarrer, Christiane, Mrowka, Ralf, Schleußner, Ekkehard, Markert, Udo R., Schmidt, André. Generation of Multicellular Breast Cancer Tumor Spheroids: Comparison of Different Protocols. Journal of mammary gland biology and neoplasia, vol.21, no.3, 89-98.

상세보기
Yoon, Hee Hun, Bhang, Suk Ho, Kim, Taeho, Yu, Taekyung, Hyeon, Taeghwan, Kim, Byung‐Soo. Dual Roles of Graphene Oxide in Chondrogenic Differentiation of Adult Stem Cells: Cell‐Adhesion Substrate and Growth Factor‐Delivery Carrier. Advanced functional materials, vol.24, no.41, 6455-6464.

상세보기
Park, Jooyeon, Kim, Yong Sook, Ryu, Seungmi, Kang, Wan Seok, Park, Subeom, Han, Jin, Jeong, Hae Chang, Hong, Byung Hee, Ahn, Youngkeun, Kim, Byung‐Soo. Graphene Potentiates the Myocardial Repair Efficacy of Mesenchymal Stem Cells by Stimulating the Expression of Angiogenic Growth Factors and Gap Junction Protein. Advanced functional materials, vol.25, no.17, 2590-2600.

상세보기
Nguyen-Ngoc, Kim-Vy, Cheung, Kevin J., Brenot, Audrey, Shamir, Eliah R., Gray, Ryan S., Hines, William C., Yaswen, Paul, Werb, Zena, Ewald, Andrew J.. ECM microenvironment regulates collective migration and local dissemination in normal and malignant mammary epithelium. Proceedings of the National Academy of Sciences of the United States of America, vol.109, no.39,

상세보기
Carey, Shawn P., Martin, Karen E., Reinhart-King, Cynthia A.. Three-dimensional collagen matrix induces a mechanosensitive invasive epithelial phenotype. Scientific reports, vol.7, 42088-.

상세보기
Costa, Elisabete C., de Melo‐Diogo, Duarte, Moreira, André F., Carvalho, Marco P., Correia, Ilídio J.. Spheroids Formation on Non‐Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches. Biotechnology journal, vol.13, no.1, 1700417-.

상세보기
Breslin, S., O'Driscoll, L.. Three-dimensional cell culture: the missing link in drug discovery. Drug discovery today, vol.18, no.5, 240-249.

상세보기
Choi, Minsuk, Yu, Seung J., Choi, Yoonjung, Lee, Hak R., Lee, Eunbeol, Lee, Eunjung, Lee, Yumi, Song, Junhyuk, Son, Jin G., Lee, Tae G., Kim, Jin Y., Kang, Sukmo, Baek, Jieung, Lee, Daeyoup, Im, Sung G., Jon, Sangyong. Polymer Thin Film–Induced Tumor Spheroids Acquire Cancer Stem Cell–like Properties. Cancer research : the official organ of the American Association for Cancer Research, Inc, vol.78, no.24, 6890-6902.

상세보기
Zanoni, Michele, Piccinini, Filippo, Arienti, Chiara, Zamagni, Alice, Santi, Spartaco, Polico, Rolando, Bevilacqua, Alessandro, Tesei, Anna. 3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained. Scientific reports, vol.6, 19103-.

상세보기
Amano, Mutsuki, Nakayama, Masanori, Kaibuchi, Kozo. Rho-Kinase/ROCK: A Key Regulator of the Cytoskeleton and Cell Polarity. Cytoskeleton, vol.67, no.9, 545-554.

상세보기
Robinson, Elizabeth E., Zazzali, Kathleen M., Corbett, Siobhan A., Foty, Ramsey A.. α5β1 integrin mediates strong tissue cohesion. Journal of cell science, vol.116, no.2, 377-386.

상세보기
Casey, Rachael C., Burleson, Kathryn M., Skubitz, Keith M., Pambuccian, Stefan E., Oegema Jr., Theodore R., Ruff Jr., Laura E., Skubitz Jr., Amy P.N.. β1-Integrins Regulate the Formation and Adhesion of Ovarian Carcinoma Multicellular Spheroids. The American journal of pathology, vol.159, no.6, 2071-2080.

상세보기
10.3892/ijo.31.6.1403
Kenny, P.A., Lee, G.Y., Myers, C.A., Neve, R.M., Semeiks, J.R., Spellman, P.T., Lorenz, K., Lee, E.H., Barcellos-Hoff, M.H., Petersen, O.W., Gray, J.W., Bissell, M.J.. The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression. Molecular Oncology, vol.1, no.1, 84-96.

상세보기
Naba, Alexandra, Clauser, Karl R, Lamar, John M, Carr, Steven A, Hynes, Richard O, Fuchs, Elaine. Extracellular matrix signatures of human mammary carcinoma identify novel metastasis promoters. eLife, vol.3, e01308-.

상세보기
Paszek, Matthew J., Zahir, Nastaran, Johnson, Kandice R., Lakins, Johnathon N., Rozenberg, Gabriela I., Gefen, Amit, Reinhart-King, Cynthia A., Margulies, Susan S., Dembo, Micah, Boettiger, David, Hammer, Daniel A., Weaver, Valerie M.. Tensional homeostasis and the malignant phenotype. Cancer cell, vol.8, no.3, 241-254.

상세보기
Mendez, Melissa G., Kojima, Shin‐Ichiro, Goldman, Robert D.. Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol.24, no.6, 1838-1851.

상세보기
Ivaska, Johanna, Vuoriluoto, Karoliina, Huovinen, Tuomas, Izawa, Ichiro, Inagaki, Masaki, Parker, Peter J. PKCɛ-mediated phosphorylation of vimentin controls integrin recycling and motility. The EMBO journal, vol.24, no.22, 3834-3845.

상세보기
Liu, Ching-Yi, Lin, Hsi-Hui, Tang, Ming-Jer, Wang, Yang-Kao. Vimentin contributes to epithelial-mesenchymal transition cancer cell mechanics by mediating cytoskeletal organization and focal adhesion maturation. Oncotarget, vol.6, no.18, 15966-15983.

상세보기
Friedl, P., Alexander, S.. Cancer Invasion and the Microenvironment: Plasticity and Reciprocity. Cell, vol.147, no.5, 992-1009.

상세보기
Bissell, Mina J., Radisky, Derek. Putting tumours in context. Nature reviews. Cancer, vol.1, no.1, 46-54.

상세보기
Mol. Cancer Res. Porter D. 362 1 2003

상세보기
Chin, Koei, de Solorzano, Carlos Ortiz, Knowles, David, Jones, Arthur, Chou, William, Rodriguez, Enrique Garcia, Kuo, Wen-Lin, Ljung, Britt-Marie, Chew, Karen, Myambo, Kenneth, Miranda, Monica, Krig, Sheryl, Garbe, James, Stampfer, Martha, Yaswen, Paul, Gray, Joe W, Lockett, Stephen J. In situ analyses of genome instability in breast cancer. Nature genetics, vol.36, no.9, 984-988.

상세보기
Ma, Xiao-Jun, Salunga, Ranelle, Tuggle, J. Todd, Gaudet, Justin, Enright, Edward, McQuary, Philip, Payette, Terry, Pistone, Maria, Stecker, Kimberly, Zhang, Brian M., Zhou, Yi-Xiong, Varnholt, Heike, Smith, Barbara, Gadd, Michelle, Chatfield, Erica, Kessler, Jessica, Baer, Thomas M., Erlander, Mark G., Sgroi, Dennis C.. Gene expression profiles of human breast cancer progression. Proceedings of the National Academy of Sciences of the United States of America, vol.100, no.10, 5974-5979.

상세보기
Hwang, E. Shelley, DeVries, Sandy, Chew, Karen L., Moore II, Dan H., Kerlikowske, Karla, Thor, Ann, Ljung, Britt-Marie, Waldman, Frederic M.. Patterns of Chromosomal Alterations in Breast Ductal Carcinoma In situ. Clinical Cancer research : an official journal of the American Association for Cancer Research, vol.10, no.15, 5160-5167.

상세보기
Lahlou, Hicham, Muller, William J. β1-integrins signaling and mammary tumor progression in transgenic mouse models: implications for human breast cancer. Breast cancer research : BCR, vol.13, no.6, 229-229.

상세보기
Park, Catherine C., Zhang, Hui, Pallavicini, Maria, Gray, Joe W., Baehner, Frederick, Park, Chong J., Bissell, Mina J.. β1 Integrin Inhibitory Antibody Induces Apoptosis of Breast Cancer Cells, Inhibits Growth, and Distinguishes Malignant from Normal Phenotype in Three Dimensional Cultures and In vivo. Cancer research : the official organ of the American Association for Cancer Research, Inc, vol.66, no.3, 1526-1535.

상세보기
Guo, Wenjun, Giancotti, Filippo G.. Integrin signalling during tumour progression. Nature reviews. Molecular cell biology, vol.5, no.10, 816-826.

상세보기
Cabodi, Sara, del Pilar Camacho-Leal, Maria, Di Stefano, Paola, Defilippi, Paola. Integrin signalling adaptors: not only figurants in the cancer story. Nature reviews. Cancer, vol.10, no.12, 858-870.

상세보기
Khan, Zareen, Marshall, John F.. The role of integrins in TGFβ activation in the tumour stroma. Cell and tissue research, vol.365, no.3, 657-673.

상세보기
Sawai, H, Okada, Y, Funahashi, H, Matsuo, Y, Takahashi, H, Takeyama, H, Manabe, T. Integrin-linked kinase activity is associated with interleukin-1α-induced progressive behavior of pancreatic cancer and poor patient survival. Oncogene, vol.25, no.23, 3237-3246.

상세보기
Neve, Richard M., Chin, Koei, Fridlyand, Jane, Yeh, Jennifer, Baehner, Frederick L., Fevr, Tea, Clark, Laura, Bayani, Nora, Coppe, Jean-Philippe, Tong, Frances, Speed, Terry, Spellman, Paul T., DeVries, Sandy, Lapuk, Anna, Wang, Nick J., Kuo, Wen-Lin, Stilwell, Jackie L., Pinkel, Daniel, Albertson, Donna G., Waldman, Frederic M., McCormick, Frank, Dickson, Robert B., Johnson, Michael D., Lippman, Marc, Ethier, Stephen, Gazdar, Adi, Gray, Joe W.. A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. Cancer cell, vol.10, no.6, 515-527.

상세보기
O'Brien, Katie M., Cole, Stephen R., Tse, Chiu-Kit, Perou, Charles M., Carey, Lisa A., Foulkes, William D., Dressler, Lynn G., Geradts, Joseph, Millikan, Robert C.. Intrinsic Breast Tumor Subtypes, Race, and Long-Term Survival in the Carolina Breast Cancer Study. Clinical Cancer research : an official journal of the American Association for Cancer Research, vol.16, no.24, 6100-6110.

상세보기
Mei, Yi, Cai, Dongyan, Dai, Xiaofeng. Modulating cancer stemness provides luminal a breast cancer cells with HER2 positive-like features. Journal of Cancer, vol.11, no.5, 1162-1169.

상세보기
Wang, Kefeng, Zhou, Changchun, Hong, Youliang, Zhang, Xingdong. A review of protein adsorption on bioceramics. Interface focus, vol.2, no.3, 259-277.

상세보기
Strathearn, K.; Rothenberg, M. E. Corning ultra-low attachment surface promotes spheroid formation in MCF-7 human breast cancer cell line ; Maine Corning Inc, 2012.
Recek, Nina, Jaganjac, Morana, Kolar, Metod, Milkovic, Lidija, Mozetič, Miran, Stana-Kleinschek, Karin, Vesel, Alenka. Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates. Molecules a journal of synthetic chemistry and natural product chemistry, vol.18, no.10, 12441-12463.

상세보기
Lee, Yu Bin, Kim, Eun Mi, Byun, Hayeon, Chang, Hyung-kwan, Jeong, Kwanghee, Aman, Zachary M., Choi, Yu Suk, Park, Jungyul, Shin, Heungsoo. Engineering spheroids potentiating cell-cell and cell-ECM interactions by self-assembly of stem cell microlayer. Biomaterials, vol.165, 105-120.

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증