[논문]Transcriptome and translatome profiles of Streptomyces species in different growth phases

Kim, Woori; Hwang, Soonkyu; Lee, Namil; Lee, Yongjae; Cho, Suhyung; Palsson, Bernhard; Cho, Byung-Kwan

doi:10.1038/s41597-020-0476-9

[해외논문] Transcriptome and translatome profiles of Streptomyces species in different growth phases 원문보기

Scientific data, v.7 = v.7 no.1, 2020년, pp.138 -

Kim, Woori (Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea) , Hwang, Soonkyu (Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea) , Lee, Namil (Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea) , Lee, Yongjae (Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea) , Cho, Suhyung (Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea) , Palsson, Bernhard (Department of Bioengineering, University of California San Diego, La Jolla, CA 92093 USA) , Cho, Byung-Kwan (Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea)

Abstract ▼ AI-Helper

Streptomyces are efficient producers of various bioactive compounds, which are mostly synthesized by their secondary metabolite biosynthetic gene clusters (smBGCs). The smBGCs are tightly controlled by complex regulatory systems at transcriptional and translational levels to effectively utilize precursors that are supplied by primary metabolism. Thus, dynamic changes in gene expression in response to cellular status at both the transcriptional and translational levels should be elucidated to directly reflect protein levels, rapid downstream responses, and cellular energy costs. In this study, RNA-Seq and ribosome profiling were performed for five industrially important Streptomyces species at different growth phases, for the deep sequencing of total mRNA, and only those mRNA fragments that are protected by translating ribosomes, respectively. Herein, 12.0 to 763.8 million raw reads were sufficiently obtained with high quality of more than 80% for the Phred score Q30 and high reproducibility. These data provide a comprehensive understanding of the transcriptional and translational landscape across the Streptomyces species and contribute to facilitating the rational engineering of secondary metabolite production.Measurement(s)transcriptome • translation • translatomeTechnology Type(s)RNA sequencing • Ribo-SeqFactor Type(s)Growth phasesSample Characteristic - OrganismStreptomyces avermitilis • Streptomyces clavuligerus • Streptomyces lividans • Streptomyces venezuelae • Streptomyces tsukubensisMachine-accessible metadata file describing the reported data: 10.6084/m9.figshare.12045603

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