[논문]Paeonia lactiflora extract suppresses cisplatin-induced muscle wasting via downregulation of muscle-specific ubiquitin E3 ligases, NF-κB signaling, and cytokine levels

Jang, Jaewoong; Lee, Hyunji; Song, Jaewon; Bae, Taehyun; Park, Minwoo; Kwon, Young V.; Lee, Doheon; Yoon, Yoosik

doi:10.1016/j.jep.2020.113403

[해외논문] Paeonia lactiflora extract suppresses cisplatin-induced muscle wasting via downregulation of muscle-specific ubiquitin E3 ligases, NF-κB signaling, and cytokine levels

Journal of ethnopharmacology, v.266, 2021년, pp.113403 -

Jang, Jaewoong (Department of Microbiology, Chung-Ang University College of Medicine) , Lee, Hyunji (Department of Microbiology, Chung-Ang University College of Medicine) , Song, Jaewon (Department of Microbiology, Chung-Ang University College of Medicine) , Bae, Taehyun (Department of Microbiology, Chung-Ang University College of Medicine) , Park, Minwoo (Research Center, EBIOGEN Inc.) , Kwon, Young V. (Department of Biochemistry, University of Washington) , Lee, Doheon (Department of Bio and Brain Engineering, KAIST) , Yoon, Yoosik (Department of Microbiology, Chung-Ang University College of Medicine)

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Abstract Ethnopharmacological relevance The dried root of Paeonia lactiflora Pall. (Radix Paeoniae) has been traditionally used to treat various inflammatory diseases in many Asian countries. Aim of the study Cisplatin is a broad-spectrum anticancer drug used in diverse types of cancer. However, muscle wasting is a common side effect of cisplatin chemotherapy. This study aimed to elucidate the effects of an ethanol extract of the root of Paeonia lactiflora Pall. (Radix Paeoniae, RP) on cisplatin-induced muscle wasting along with its molecular mechanism. Material and methods C57BL/6 mice were intraperitoneally injected with cisplatin and orally treated with RP. Megestrol acetate was used as a comparator drug. Skeletal muscle mass was measured as the weight of gastrocnemius and quadriceps muscles, and skeletal muscle function was measured by treadmill running time and grip strength. Skeletal muscle tissues were analyzed by RNAseq, western blotting, ELISA, and immunofluorescence microscopy. Results In mice treated with cisplatin, skeletal muscle mass and skeletal muscle function were significantly reduced. However, oral administration of RP significantly restored skeletal muscle mass and function in the cisplatin-treated mice. In the skeletal muscle tissues of the cisplatin-treated mice, RP downregulated NF-κB signaling and cytokine levels. RP also downregulated muscle-specific ubiquitin E3 ligases, resulting in the restoration of myosin heavy chain (MyHC) and myoblast determination protein (MyoD), which play crucial roles in muscle contraction and muscle differentiation, respectively. Conclusion RP restored skeletal muscle function and mass in cisplatin-treated mice by restoring the muscle levels of MyHC and MyoD proteins via downregulation of muscle-specific ubiquitin E3 ligases as well as muscle NF-κB signaling and cytokine levels. Highlights Paeonia lactiflora extract restores muscle mass/function in cisplatin-treated mice. It attenuates NF-κB signaling and cytokine levels in skeletal muscle. It also downregulates muscle-specific ubiquitin E3 ligases, MuRF1 and MAFbx. It restores myosin heavy chain and MyoD in skeletal muscle. It may be used to develop therapeutics against chemotherapy-induced muscle wasting. Graphical abstract [DISPLAY OMISSION]

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J. Ethnopharmacol. Bae 246 2020 10.1016/j.jep.2019.112222 Paeonia lactiflora root extract suppresses cancer cachexia by down-regulating muscular NF-kappaB signalling and muscle-specific E3 ubiquitin ligases in cancer-bearing mice

상세보기
Cochrane Database Syst. Rev. Berenstein 2005 2005 10.1002/14651858.CD004310.pub2 Megestrol acetate for the treatment of anorexia-cachexia syndrome

상세보기
Am. J. Physiol. Endocrinol. Metab. Bodine 307 E469 2014 10.1152/ajpendo.00204.2014 Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1

상세보기
Science Bodine 294 1704 2001 10.1126/science.1065874 Identification of ubiquitin ligases required for skeletal muscle atrophy

상세보기
Cell Cai 119 285 2004 10.1016/j.cell.2004.09.027 IKKbeta/NF-kappaB activation causes severe muscle wasting in mice

상세보기
Bio Protoc Castro 7 2017 10.21769/BioProtoc.2237 Evaluation of muscle performance in mice by treadmill exhaustion test and whole-limb grip strength Assay

상세보기
Integr. Canc. Ther. Chen 15 40 2016 10.1177/1534735415596424 Meta-analysis of oxaliplatin-based chemotherapy combined with traditional medicines for colorectal cancer: contributions of specific plants to tumor response

상세보기
Eur. J. Transl. Myol. Coletti 28 7587 2018 10.4081/ejtm.2018.7587 Chemotherapy-induced muscle wasting: an update

상세보기
Int. J. Mol. Sci. Conte 21 1242 2020 10.3390/ijms21041242 Cisplatin-induced skeletal muscle dysfunction: mechanisms and counteracting therapeutic strategies

상세보기
J. Cachexia Sarcopenia Muscle Daly 9 315 2018 10.1002/jcsm.12267 Loss of skeletal muscle during systemic chemotherapy is prognostic of poor survival in patients with foregut cancer

상세보기
Curr. Opin. Clin. Nutr. Metab. Care Frost 8 255 2005 10.1097/01.mco.0000165003.16578.2d Skeletal muscle cytokines: regulation by pathogen-associated molecules and catabolic hormones

상세보기
Front. Pharmacol. He 2 10 2011 10.3389/fphar.2011.00010 Anti-inflammatory and immunomodulatory effects of paeonia lactiflora pall., a traditional Chinese herbal medicine

상세보기
Curr. Opin. Clin. Nutr. Metab. Care Jagoe 4 183 2001 10.1097/00075197-200105000-00003 What do we really know about the ubiquitin-proteasome pathway in muscle atrophy?

상세보기
Sci. Rep. Jang 7 41612 2017 10.1038/srep41612 LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974

상세보기
J. Ethnopharmacol. Liu 140 614 2012 10.1016/j.jep.2012.01.047 Old formula, new Rx: the journey of PHY906 as cancer adjuvant therapy

상세보기
Signal. Transduct. Target Ther. Liu 2 2017 10.1038/sigtrans.2017.23 NF-kappaB signaling in inflammation

상세보기
Cochrane Database Syst. Rev. Ruiz Garcia 2013 2013 Megestrol acetate for treatment of anorexia-cachexia syndrome

상세보기
Toxicol. Appl. Pharmacol. Sakai 278 190 2014 10.1016/j.taap.2014.05.001 Mechanisms of cisplatin-induced muscle atrophy

상세보기
Annu. Rev. Pharmacol. Toxicol. Schwartz 49 73 2009 10.1146/annurev.pharmtox.051208.165340 Targeting proteins for destruction by the ubiquitin system: implications for human pathobiology

상세보기
Nutr. Canc. Shadfar 63 749 2011 10.1080/01635581.2011.563032 Oral resveratrol therapy inhibits cancer-induced skeletal muscle and cardiac atrophy in vivo

상세보기
Proc. Natl. Acad. Sci. U.S.A. Subramanian 102 15545 2005 10.1073/pnas.0506580102 Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles

상세보기
Nucleic Acids Res. Szklarczyk 45 D362 2017 10.1093/nar/gkw937 The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible

상세보기

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[해외논문] Paeonia lactiflora extract suppresses cisplatin-induced muscle wasting via downregulation of muscle-specific ubiquitin E3 ligases, NF-κB signaling, and cytokine levels

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[해외논문] Paeonia lactiflora extract suppresses cisplatin-induced muscle wasting via downregulation of muscle-specific ubiquitin E3 ligases, NF-κB signaling, and cytokine levels

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