[논문]Vitamin E reduces spasms caused by prenatal stress by lowering calpain expression

Kwon, Hyeok Hee; Lee, Jin-Seok; Park, Hyewon; Shin, Juhee; Yin, Yuhua; Shin, Nara; Shin, Hyo Jung; Hwang, Jeong-Ah; Kim, Dong Woon; Kang, Joon Won

doi:10.1016/j.yebeh.2020.107609

[해외논문] Vitamin E reduces spasms caused by prenatal stress by lowering calpain expression

Epilepsy & behavior : E&B, v.114 pt.A, 2021년, pp.107609 -

Kwon, Hyeok Hee (Department of Medical Science, School of Medicine, Chungnam National University) , Lee, Jin-Seok (Liver and Immunology Research Center, Oriental Medical College of Daejeon University) , Park, Hyewon (Department of Medical Science, School of Medicine, Chungnam National University) , Shin, Juhee (Department of Medical Science, School of Medicine, Chungnam National University) , Yin, Yuhua (Department of Medical Science, School of Medicine, Chungnam National University) , Shin, Nara (Department of Medical Science, School of Medicine, Chungnam National University) , Shin, Hyo Jung (Department of Medical Science, School of Medicine, Chungnam National University) , Hwang, Jeong-Ah (Department of Medical Science, School of Medicine, Chungnam National University) , Kim, Dong Woon (Department of Medical Science, School of Me) , Kang, Joon Won

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Abstract Background Prenatal stress increases the susceptibility of infants to seizures and is known to be associated with oxidative stress. Recent studies suggest that vitamin E has beneficial effects in various neurological diseases due to its antioxidant properties. In this study, we investigated the relationship between prenatal stress and vitamin E treatment on N-methyl-D-aspartate (NMDA)-induced spasms. Methods We used pregnant female Sprague Dawley rats and induced prenatal stress with an injection of betamethasone on G15. They were then treated orally with 200 mg/kg vitamin E or saline twice a day from G15–G21. On postnatal day 15, NMDA was administered to trigger spasms in offspring. The total number of spasms and latency to the first spasm were recorded. We also measured oxidative stress in the medial cortex using western blot, and calpain activity, thiobarbituric acid reactive substances (TBARS), glutathione (GSH)/GSH/glutathione disulfide (GSSG), superoxide dismutase (SOD) activity, catalase activity, and nitric oxide (NO) assays. Results We observed that rats treated with vitamin E while exposed to prenatal stress demonstrated reduced total number and frequency of spasms. Expression of glutamate decarboxylase 67 (GAD67) and K+/Cl− co-transporter (KCC2) were reduced after prenatal stress; this recovered in the vitamin E treated group. Further, expression of calpain 2 was decreased and various markers of oxidative stress (malondialdehyde (MDA), GSH/GSSG, SOD, catalase, and NO) were reduced in the vitamin E treated group. Conclusions Our results provide evidence that vitamin E lowers oxidative stress and decreases seizure susceptibility in rat offspring exposed to prenatal stress. Given the well-known safety profile of vitamin E, these results indicate its potential as a strategy for preventing seizures. Highlights Vitamin E reduced susceptibility to N-methyl-D-aspartate (NMDA)-induced spasms in prenatally stressed rats. Vitamin E rescued K+/Cl− co-transporter (KCC2), glutamate decarboxylase (GAD67), and calpain expressions in prenatally stressed rats. Dihydroethidium (DHE), malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), glutathione (GSH)/GSH/glutathione disulfide (GSSG), and catalase -mediated reactive oxygen species (ROS) decreased in the treated rats. Graphical abstract [DISPLAY OMISSION]

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참고문헌 (34)

Pediatrics Aaberg 139 e20163908 2017 10.1542/peds.2016-3908 Incidence and prevalence of childhood epilepsy: a nationwide cohort study

상세보기
Neurosci Biobehav Rev Glover 35 17 2010 10.1016/j.neubiorev.2009.11.008 Prenatal stress and the programming of the HPA axis

상세보기
CNS Neurosci Ther Cepeda 21 71 2015 10.1111/cns.12370 Special issue: basic mechanisms of pediatric epilepsy

상세보기
Neurochem Int Baek 97 57 2016 10.1016/j.neuint.2016.05.006 Altered expression of KCC2 in GABAergic interneuron contributes prenatal stress-induced epileptic spasms in infant rat

상세보기
Korean J Pediatr Kwon 61 150 2018 10.3345/kjp.2018.61.5.150 Long-term prenatal stress increases susceptibility of N-methyl-D-aspartic acid-induced spasms in infant rats

원문보기 상세보기
Int J Dev Neurosci Tavassoli 31 790 2013 10.1016/j.ijdevneu.2013.09.010 Effect of prenatal stress on density of NMDA receptors in rat brain

상세보기
Exp Neurobiol Kwon 28 529 2019 10.5607/en.2019.28.4.529 Calpain-2 as a treatment target in prenatal stress-induced epileptic spasms in infant rats

상세보기
Neurochem Res Song 34 739 2009 10.1007/s11064-008-9838-y Prenatal stress causes oxidative damage to mitochondrial DNA in hippocampus of offspring rats

상세보기
10.1111/j.1528-1167.2011.03357.x Yum MS, Chachua T, Veliskova J, Velisek L. Prenatal stress promotes development of spasms in infant rats. Epilepsia 2012;53:e46-9. https://doi.org/10.1111/j.1528-1167.2011.03357.x.

상세보기
10.1093/ajcn/84.6.1442 Scholl TO, Chen X, Sims M, Stein TP. Vitamin E: maternal concentrations are associated with fetal growth. Am J Clin Nutr 2006;84:1442-8. https://doi.org/10.1093/ajcn/84.6.1442.

상세보기
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Ambrogini 1865 1098 2019 10.1016/j.bbadis.2019.01.026 Excitotoxicity, neuroinflammation and oxidant stress as molecular bases of epileptogenesis and epilepsy-derived neurodegeneration: the role of vitamin E

상세보기
Adv Biomed Res Mehvari 5 36 2016 10.4103/2277-9175.178780 Effects of Vitamin E on seizure frequency, electroencephalogram findings, and oxidative stress status of refractory epileptic patients

상세보기
10.1111/j.1528-1167.2011.03220.x Chachua T, Yum MS, Veliskova J, Velisek L. Validation of the rat model of cryptogenic infantile spasms. Epilepsia 2011;52:1666-77. https://doi.org/10.1111/j.1528-1167.2011.03220.x.

상세보기
10.1002/ana.21082 Velisek L, Jehle K, Asche S, Veliskova J. Model of infantile spasms induced by N-methyl-D-aspartic acid in prenatally impaired brain. Ann Neurol 2007;61:109-19. https://doi.org/10.1002/ana.2108213.

상세보기
Antioxid Redox Signal Shin 30 1601 2019 10.1089/ars.2017.7280 Uncoupled endothelial nitric oxide synthase enhances p-tau in chronic traumatic encephalopathy mouse model

상세보기
10.1016/S0014-5793(02)02934-4 Tseng HC, Zhou Y, Shen Y, Tsai LH. A survey of Cdk5 activator p35 and p25 levels in Alzheimer's disease brains. FEBS Lett 2002;523:58-62. https://doi.org/10.1016/s0014-5793(02)02934-4.

상세보기
10.1111/epi.16305 Osborne JP, Edwards SW, Dietrich Alber F, Hancock E, Johnson AL, Kennedy CR, et al. participating investigators. The underlying etiology of infantile spasms (West syndrome): Information from the International Collaborative Infantile Spasms Study (ICISS). Epilepsia 2019;60:1861-9. https://doi.org/10.1111/epi.16305.

상세보기
10.3390/antiox9040313 Espinos C, Galindo MI, Garcia-Gimeno MA, Ibanez-Cabellos JS, Martinez-Rubio D, Millan JM, et al. Oxidative stress, a crossroad between rare diseases and neurodegeneration. Antioxidants (Basel) 2020;9:313. https://doi.org/10.3390/antiox9040313.

상세보기
Free Radical Biol Med Jiang 72 76 2014 10.1016/j.freeradbiomed.2014.03.035 Natural forms of vitamin E: metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy

상세보기
10.1002/14651858.CD004069.pub3 Rumbold A, Ota E, Hori H, Miyazaki C, Crowther CA. Vitamin E supplementation in pregnancy. Cochrane Database Syst Rev 2015;9:CD004069. https://doi.org/10.1002/14651858.CD004069.pub3.

상세보기
10.1093/ajcn/48.3.612 Bendich A, Machlin LJ. Safety of oral intake of vitamin E. Am J Clin Nutr 1988;48:612-9. https://doi.org/10.1093/ajcn/48.3.612.

상세보기
Physiol Behav Alzoubi 206 200 2019 10.1016/j.physbeh.2019.04.011 Vitamin E modifies high-fat diet-induced reduction of seizure threshold in rats: Role of oxidative stress

상세보기
Neurophysiology Yorulmaz 42 349 2011 10.1007/s11062-011-9168-6 Effect of vitamin E on blood-brain barrier permeability in aged rats with PTZ-induced convulsions

상세보기
J Neurosci Chamma 33 15488 2013 10.1523/JNEUROSCI.5889-12.2013 Activity-dependent regulation of the K/Cl transporter KCC2 membrane diffusion, clustering, and function in hippocampal neurons

상세보기
J Neurosci Wright 37 5447 2017 10.1523/JNEUROSCI.2164-16.2017 Neuronal chloride regulation via KCC2 is modulated through a GABA B receptor protein complex

상세보기
CNS Neurosci Ther Khazipov 21 83 2015 10.1111/cns.12353 Depolarizing GABA and developmental epilepsies

상세보기
10.1111/j.1460-9568.2005.04084.x Lee H, Chen CX, Liu YJ, Aizenman E, Kandler K. KCC2 expression in immature rat cortical neurons is sufficient to switch the polarity of GABA responses. Eur J Neurosci 2005;21:2593-9. https://doi.org/10.1111/j.1460-9568.2005.04084.x.

상세보기
J Neurosci Puskarjov 32 11356 2012 10.1523/JNEUROSCI.6265-11.2012 Activity-dependent cleavage of the K-Cl cotransporter KCC2 mediated by calcium-activated protease calpain

상세보기
Neuroscience Yu 178 13 2011 10.1016/j.neuroscience.2011.01.026 Calpain-mediated regulation of stargazin in adult rat brain

상세보기
Mol Neurobiol Zadran 42 143 2010 10.1007/s12035-010-8145-1 Regulation of Calpain-2 in neurons: implications for synaptic plasticity

상세보기
Nat Neurosci Lee 14 736 2011 10.1038/nn.2806 NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents

상세보기
10.3390/ijms21062109 Su BC, Li CC, Horng JL, Chen JY. Calcium-dependent calpain activation-mediated mitochondrial dysfunction and oxidative stress are required for cytotoxicity of epinecidin-1 in human synovial sarcoma SW982 cells. Int J Mol Sci 2020;21:2109. https://doi.org/10.3390/ijms21062109

상세보기
Seizure Feng 20 395 2011 10.1016/j.seizure.2011.01.010 Overexpression of mu-calpain in the anterior temporal neocortex of patients with intractable epilepsy correlates with clinicopathological characteristics

상세보기
Brain Res Ray 852 326 2000 10.1016/S0006-8993(99)02148-4 Oxidative stress and Ca2+ influx upregulate calpain and induce apoptosis in PC12 cells

상세보기

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