[논문]Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency

Choi, In Young; Lim, HoTae; Huynh, Alex; Schofield, James; Cho, Hyeon Jin; Lee, Hosuk; Andersen, Peter; Shin, Joo Heon; Heo, Won Do; Hyun, Sang-Hwan; Kim, Yong Jun; Oh, Yohan; Kim, Hyesoo; Lee, Gabsang

doi:10.1016/j.biomaterials.2020.120222

[해외논문] Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency 원문보기

Biomaterials, v.269, 2021년, pp.120222 -

Choi, In Young (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Lim, HoTae (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Huynh, Alex (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Schofield, James (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Cho, Hyeon Jin (Lieber Institute for Brain Development, Johns Hopkins Medical Campus) , Lee, Hosuk (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Andersen, Peter (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Shin, Joo Heon (Lieber Institute for Brain Development, Johns Hopkins Medical Campus) , Heo, Won Do (Center for Cognition and Sociality, Institute for Basic Science) , Hyun, Sang-Hwan (Institute for Cell Engineering, Johns Hopkins University School of Medicine) , Kim, Yong Jun (De) , Oh, Yohan , Kim, Hyesoo , Lee, Gabsang

Abstract ▼ AI-Helper

Abstract Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture.

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