[논문]Foxp3 plasmid-encapsulated PLGA nanoparticles attenuate pain behavior in rats with spinal nerve ligation

Shin, Juhee; Yin, Yuhua; Kim, Do Kyung; Lee, Sun Yeul; Lee, Wonhyung; Kang, Joon Won; Kim, Dong Woon; Hong, Jinpyo

doi:10.1016/j.nano.2019.02.023

Foxp3 plasmid-encapsulated PLGA nanoparticles attenuate pain behavior in rats with spinal nerve ligation

Nanomedicine : nanotechnology, biology and medicine, v.18, 2019년, pp.90 - 100

Shin, Juhee (Department of Medical Science, Chungnam National University School of Medicine) , Yin, Yuhua (Department of Medical Science, Chungnam National University School of Medicine) , Kim, Do Kyung (Department of Anatomy, College of Medicine, Konyang University) , Lee, Sun Yeul (Department of Anesthesia and Pain Medicine, Chungnam National University Hospital) , Lee, Wonhyung (Department of Anesthesia and Pain Medicine, Chungnam National University Hospital) , Kang, Joon Won (Department of Medical Science, Chungnam National University School of Medicine) , Kim, Dong Woon (Department of Medical Science, Chungnam National University School of Medicine) , Hong, Jinpyo (Department of Anatomy, Brain Research Institute, Chungnam National University School of Medicine)

Abstract ▼ AI-Helper

Abstract Microglia play a critical role in neuropathic pain. Since upregulated Foxp3 in microglia enhances tissue repair by resolving neuroinflammation in excitotoxin-induced neuronal death, it may attenuate neuropathic pain in a similar manner. Therefore, this study tests whether Foxp3 introduced with poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles (Foxp3 NPs) can alleviate neuropathic pain by inhibiting microglia activity. The prepared Foxp3 NPs had an anti-inflammatory effect on lipopolysaccharide-stimulated BV2 cells in vitro, and localized to spinal microglia in vivo. Further, the Foxp3 NPs significantly attenuated pain behavior induced by spinal nerve ligation in rats for 7 days by suppressing microglial activity, followed by the downregulation of pro-nociceptive genes and the upregulation of anti-nociceptive genes in the spinal dorsal horn. Collectively, these data suggest that Foxp3 NPs effectively relieve neuropathic pain in animals by reducing microglia activity and subsequent modulation of neuroinflammation, and may be of therapeutic value in the treatment of neuropathic pain. Graphical Abstract Neuropathic pain was induced by lumbar 5 spinal nerve ligation in rats. NC or Foxp3 plasmids-encapsulated PLGA nanoparticles (NC NPs or Foxp3 NPs) were then administered intrathecally into the spinal subarachnoid space of rats with neuropathic pain. Pain behavior in von Frey filaments tests was significantly attenuated in rats with the Foxp3 NPs compared with the NC NPs. [DISPLAY OMISSION]

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Foxp3 plasmid-encapsulated PLGA nanoparticles attenuate pain behavior in rats with spinal nerve ligation

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Foxp3 plasmid-encapsulated PLGA nanoparticles attenuate pain behavior in rats with spinal nerve ligation

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