[논문]Production of semi-biosynthetic nepetalactone in yeast

Billingsley, John M; Anguiano, Jose L; Tang, Yi

doi:10.1007/s10295-019-02199-x

[해외논문] Production of semi-biosynthetic nepetalactone in yeast 원문보기

Journal of industrial microbiology & biotechnology, v.46 no.9/10, 2019년, pp.1365 - 1370

Billingsley, John M (grid.19006.3e 0000 0000 9632 6718 Department of Chemical and Biomolecular Engineering University of California 90095 Los Angeles CA USA) , Anguiano, Jose L (grid.19006.3e 0000 0000 9632 6718 Department of Chemical and Biomolecular Engineering University of California 90095 Los Angeles CA USA) , Tang, Yi (grid.19006.3e 0000 0000 9632 6718 Department of Chemical and Biomolecular Engineering University of California 90095 Los Angeles CA USA)

Abstract ▼ AI-Helper

AbstractMicrobial-based production of natural products provides a promising alternative to synthetic production and isolation from the native producer. The recently discovered NEPS1 cyclase/oxidase completes the biosynthetic pathway to nepetalactone, a biologically relevant iridoid known as both an insect repellent and cat attractant. In this work, we employ yeast-based whole-cell biocatalysis to produce semi-biosynthetic nepetalactone from a low-cost precursor via a four-step enzymatic process. The dependence of product yield on bioprocess parameters ranging from induction of gene expression to substrate loading was investigated. Subsequent factorial design and response surface methodology optimization approach enabled a 5.8-fold increase in nepetalactone titer to 153 mg/L. Our study provides insights into strategies for operating plasmid-based bioconversion of a fed substrate and sets the stage for scalable, microbial synthesis of nepetalactone.

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[해외논문] Production of semi-biosynthetic nepetalactone in yeast 원문보기

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