[논문]Electrochemical Immunoassay for Determination of Glycated Albumin using Nanozymes

Choi, Hyun; Son, Seong Eun; Hur, Won; Tran, Van-Khue; Lee, Han; Been; Park, Yosep; Han, Do Kyoung; Seong, Gi Hun

doi:10.1038/s41598-020-66446-3

[해외논문] Electrochemical Immunoassay for Determination of Glycated Albumin using Nanozymes 원문보기

Scientific reports, v.10, 2020년, pp.9513 -

Choi, Hyun (Department of Bionano Engineering, Hanyang University, Ansan, 426 –) , Son, Seong Eun (791 South Korea) , Hur, Won (Department of Bionano Engineering, Hanyang University, Ansan, 426 –) , Tran, Van-Khue (791 South Korea) , Lee, Han Been (Department of Bionano Engineering, Hanyang University, Ansan, 426 –) , Park, Yosep (791 South Korea) , Han, Do Kyoung (Department of Bionano Engineering, Hanyang University, Ansan, 426 –) , Seong, Gi Hun (791 South Korea)

Abstract ▼ AI-Helper

We developed a new nanozyme-based electrochemical immunoassay method for the monitoring of glycated albumin (GA) known to reflect short-term glycaemic levels. For this study, we synthesized urchin-like Pt nanozymes (uPtNZs) and applied them to colorimetric and electrochemical assays for sensitive determination of GA in total human serum albumin (tHSA) using 3,3′,5,5′-tetramethylbenzidine (TMB) and thionine as substrates, respectively. The uPtNZs showed peroxidase-mimic activity in the presence of hydrogen peroxide. Boronic acid (BA)-agarose bead was used to capture GA through specific cis-diol interactions. uPtNZs were modified with GA antibody (GA-Ab) to form sandwich complexes with GA/BA-agarose bead. The amount of Ab-uPtNZ/GA/BA-agarose bead complex increased with increasing percentage of GA in 50 mg/mL tHSA. The colorimetric assay exhibited linearity from 0.02 to 10% (10 µg/mL – 5 mg/mL) GA with an LOD of 0.02% (9.2 µg/mL). For electrochemical assay, GA was detected from 0.01 to 20% (5 µg/mL – 10 mg/mL) with an LOD of 0.008% (3.8 µg/mL). The recovery values of measured GA in human plasma samples were from 106 to 107%. These results indicate that electrochemical assay using uPtNZs is a promising method for determining GA.

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DOI : 10.1038/s41598-020-66446-3 [무료]
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