[논문]Heterologous activation of the Hevea PEP16 promoter in the rubber-producing laticiferous tissues of Taraxacum kok-saghyz

Ganesh, Irisappan; Choi, Sang Chul; Bae, Sung Woo; Park, Jong-Chan; Ryu, Stephen Beungtae

doi:10.1038/s41598-020-67328-4

[해외논문] Heterologous activation of the Hevea PEP16 promoter in the rubber-producing laticiferous tissues of Taraxacum kok-saghyz 원문보기

Scientific reports, v.10, 2020년, pp.10844 -

Ganesh, Irisappan (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea) , Choi, Sang Chul (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea) , Bae, Sung Woo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea) , Park, Jong-Chan (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea) , Ryu, Stephen Beungtae (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea)

Abstract ▼ AI-Helper

Hevea brasiliensis, the most abundant rubber crop, is used widely for the commercial production of natural rubber. To reduce the risk of a shortage in the supply of natural rubber that may arise from a single major rubber crop, rubber dandelion (Taraxacum kok-saghyz) has been developed as an alternative rubber-producing crop by using a transgenic approach. However, it is necessary to identify a suitable promoter for the transfer of rubber biosynthesis-related genes to the species. In this study, the promoter region of H. brasiliensis PEP16, which was isolated as a potentially important component in rubber biosynthesis, was sequenced and a pPEP16::GUS fusion construct was introduced into T. kok-saghyz. Histological and fluorometric studies using transgenic T. kok-saghyz plants indicated that the HbPEP16 promoter was highly activated in a laticiferous tissue-specific manner under normal growth conditions and that promoter activation was tightly regulated by various hormones and external signals. These findings suggested that the HbPEP16 promoter may be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of T. kok-saghyz.

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