[논문]Pushbutton-activated microfluidic dropenser for droplet digital PCR

Park, Juhwan; Lee, Kyoung G.; Han, Dong Hyun; Lee, Ji-Soo; Lee, Seok Jae; Park, Je-Kyun

doi:10.1016/j.bios.2021.113159

[해외논문] Pushbutton-activated microfluidic dropenser for droplet digital PCR

Biosensors & bioelectronics, v.181, 2021년, pp.113159 -

Park, Juhwan (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Lee, Kyoung G. (Nanobio Application Team, National Nanofab Center (NNFC)) , Han, Dong Hyun (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Lee, Ji-Soo (TNS Co., Ltd.) , Lee, Seok Jae (Nanobio Application Team, National Nanofab Center (NNFC)) , Park, Je-Kyun (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST))

Abstract ▼ AI-Helper

Abstract Here, we report a portable microfluidic device to generate and dispense droplets simply operated by pushbutton for droplet digital polymerase chain reaction (ddPCR), which is named pushbutton-activated microfluidic dropenser (droplet dispenser) (PAMD). After loading the PCR mixtures and the droplet generation oil to PAMD, digitized PCR mixtures are prepared in PCR tubes after the actuation of a pushbutton. Multiple droplet generation units are simultaneously operated by a single pushbutton, and the size of droplets is controllable by adjusting the geometry of the droplet generation channel. To examine the performance of PAMD, digitized PCR mixtures containing genomic DNA of Escherichia coli (E. coli) O157:H7 prepared by PAMD were assessed by a fluorescence signal analyzer after PCR with a thermal cycler. As a result, PAMD can produce analytical droplets for ddPCR as much as a conventional droplet generator even though any external equipment is not required. Highlights A pushbutton-activated microfluidic device was developed for droplet digital PCR. Monodisperse droplets were generated by adjusting the geometry of the microchannel. Conventional droplet generator can be replaceable with comparable performance. Genomic DNA of Escherichia coli O157:H7 was detected using a proposed device.

Keyword

참고문헌 (32)

PloS One Alteri 15 1 2020 10.1371/journal.pone.0236311 Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients

상세보기
Lab Chip Ball 16 4436 2016 10.1039/C6LC01104G A simple check valve for microfluidic point of care diagnostics

상세보기
Methods Chen 5 984 2018 A hand-held, power-free microfluidic device for monodisperse droplet generation
Lab Chip Chen 17 235 2017 10.1039/C6LC01305H Centrifugal micro-channel array droplet generation for highly parallel digital PCR

상세보기
Lab Chip Chong 16 35 2016 10.1039/C5LC01012H Active droplet generation in microfluidics

상세보기
Anal. Chem. Hindson 83 8604 2011 10.1021/ac202028g High-throughput droplet digital PCR system for absolute quantitation of DNA copy number

상세보기
Nat. Methods Hindson 10 1003 2013 10.1038/nmeth.2633 Absolute quantification by droplet digital PCR versus analog real-time PCR

상세보기
Lab Chip Iwai 14 3790 2014 10.1039/C4LC00500G Finger-powered microfluidic systems using multilayer soft lithography and injection molding processes

상세보기
Biochip J Jang 11 329 2017 10.1007/s13206-017-1410-x Droplet-based digital PCR system for detection of single-cell level of foodborne pathogens

상세보기
Lab Chip Kahkeshani 16 2474 2016 10.1039/C6LC00645K Drop formation using ferrofluids driven magnetically in a step emulsification device

상세보기
Anal. Chem. Kahkeshani 90 8881 2018 10.1021/acs.analchem.8b00958 Ferrodrop dose-optimized digital quantification of biomolecules in low-volume samples

상세보기
TrAC Trends Anal. Chem. (Reference Ed.) Kang 58 145 2014 10.1016/j.trac.2014.03.006 Droplet microfluidics for single-molecule and single-cell analysis in cancer research, diagnosis and therapy

상세보기
Analyst Kim 141 5753 2016 10.1039/C6AN00681G A smart multi-pipette for hand-held operation of microfluidic devices

상세보기
J. Contr. Release Kim 322 337 2020 10.1016/j.jconrel.2020.03.046 On-site fabrication of injectable 131I-labeled microgels for local radiotherapy

상세보기
Biomicrofluidics Langer 12 2018 Micropipette-powered droplet based microfluidics

상세보기
Micro Nano Syst. Lett. Lee 6 1 2018 10.1186/s40486-018-0063-4 Finger-triggered portable PDMS suction cup for equipment-free microfluidic pumping

상세보기
Soft Matter Lee 16 614 2020 10.1039/C9SM01426H Simultaneous electric production and sizing of emulsion droplets in microfluidics

상세보기
Microfluid. Nanofluidics Li 18 1067 2015 10.1007/s10404-014-1497-5 A self-powered microfluidic monodispersed droplet generator with capability of multi-sample introduction

상세보기
Lab Chip Li 12 1587 2012 10.1039/c2lc40125h Squeeze-chip: a finger-controlled microfluidic flow network device and its application to biochemical assays

상세보기
Adv. Biosyst. Matuła 4 2020 Single-cell analysis using droplet microfluidics

상세보기
Micromachines Nguyen 8 186 2017 10.3390/mi8060186 Recent advances and future perspectives on microfluidic liquid handling

상세보기
Lab Chip Park 20 3922 2020 10.1039/D0LC00803F Acoustofluidic generation of droplets with tunable chemical concentrations

상세보기
Lab Chip Park 20 3346 2020 10.1039/D0LC00432D Reciprocating flow-assisted nucleic acid purification using a finger-actuated microfluidic device

상세보기
Lab Chip Park 19 2973 2019 10.1039/C9LC00422J Integrated microfluidic pumps and valves operated by finger actuation

상세보기
Lab Chip Park 18 1215 2018 10.1039/C7LC01128H Finger-actuated microfluidic device for the blood cross-matching test

상세보기
Lab Chip Ren 15 121 2015 10.1039/C4LC00798K Breakup dynamics and dripping-to-jetting transition in a Newtonian/shear-thinning multiphase microsystem

상세보기
Phys. Rev. Appl. Roudini 14 1 2020 10.1103/PhysRevApplied.14.014071 Droplet generation in standing-surface-acoustic-wave nebulization at controlled air humidity

상세보기
Lab Chip Schuler 15 2759 2015 10.1039/C5LC00291E Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA

상세보기
Lab Chip Schuler 16 208 2016 10.1039/C5LC01068C Digital droplet PCR on disk

상세보기
Anal. Chem. Tanaka 87 4134 2015 10.1021/ac503169h Hands-off preparation of monodisperse emulsion droplets using a poly(dimethylsiloxane) microfluidic chip for droplet digital PCR

상세보기
Lab Chip Xu 15 867 2015 10.1039/C4LC01319K Simultaneous metering and dispensing of multiple reagents on a passively controlled microdevice solely by finger pressing

상세보기
Lab Chip Zhu 17 34 2017 10.1039/C6LC01018K Passive and active droplet generation with microfluidics: a review

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] Pushbutton-activated microfluidic dropenser for droplet digital PCR

Abstract ▼ AI-Helper

Keyword

참고문헌 (32)

이 논문을 인용한 문헌

연구과제 타임라인

활용도 분석정보

활용도 Top5 논문

관련 콘텐츠

원문 URL 링크

연관된 기능

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

[해외논문] Pushbutton-activated microfluidic dropenser for droplet digital PCR

Abstract ▼ AI-Helper

Keyword

참고문헌 (32)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

활용도 분석정보

활용도 Top5 논문 더보기

관련 콘텐츠

원문 URL 링크

연관된 기능

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

활용도 Top5 논문