본 연구는 순수 메탄생성균의 배양시 5 종류의 화학물질 첨가에 따른 메탄생성 억제효과를 조사하고자 실행되었다. 메탄생성에 억제효과가 있다고 알려져 있는 2-bromoethane sulfonic acid(BES), 3-bromopropane sulfonic acid(BPS), lumazine, propynoic acid 및 ethyl 2-butynoate를 순수 메탄생성균인 Methanobacterium formicicum KOR-1, Methanoculleus bourgensis KOR-2, Methanosarcina mazei KOR-3, KOR-7, KOR-9 및 KOR-10, Methanobacterium formicicum KOR-12, Methanosarcina vacuolata(DSM 1232), Methanobacterium bryantii(ATCC 33272), Methanosarcina mazei(ATCC BAA159) 및 Methanosarcina barkeri(ATCC 43240)의 배양배지에 첨가하여 6 및 10일 간 39℃ 혐기적 조건에서 배양하여 메탄생성량을 조사하였다. BES, lumazaine과 ethyl 2-butynoate는 메탄생성균들의 메탄생성을 억제하였으며 첨가수준이 증가함으로써 억제력이 증가하였다. 그러나, BPS와 propynoic acid는 모든 메탄생성균의 메탄생성에 억제효과가 없었다. Methanosarcina 속의 균들이 BES, lumazaine 및 ethyl 2-butynoate에 대해 가장 저항성이 높았고, Methanobacteriales 목에 속하는 균들이 가장 작은 저항성을 나타내었다. 본 연구에서 이용된 화학물질들에 대한 메탄생성균들의 저항성은 메탄생성균들의 세포막구조의 차이에 의한 억제물질의 세포내 접근성, 억제물질의 메탄생성 pathway의 작용단계에 따른 기질이용성, H2의 분압의 변화에 따른 억제물질의 전자수용체로써 작용효과 등의 차이로 다양하였다. 결론적으로, 혼합 혐기 미생물 생태계에서 메탄생성균에 대해 메탄생성억제물질을 사용할 때 메탄생성억제효과의 차이를 충분히 고려해야할 것이다.
본 연구는 순수 메탄생성균의 배양시 5 종류의 화학물질 첨가에 따른 메탄생성 억제효과를 조사하고자 실행되었다. 메탄생성에 억제효과가 있다고 알려져 있는 2-bromoethane sulfonic acid(BES), 3-bromopropane sulfonic acid(BPS), lumazine, propynoic acid 및 ethyl 2-butynoate를 순수 메탄생성균인 Methanobacterium formicicum KOR-1, Methanoculleus bourgensis KOR-2, Methanosarcina mazei KOR-3, KOR-7, KOR-9 및 KOR-10, Methanobacterium formicicum KOR-12, Methanosarcina vacuolata(DSM 1232), Methanobacterium bryantii(ATCC 33272), Methanosarcina mazei(ATCC BAA159) 및 Methanosarcina barkeri(ATCC 43240)의 배양배지에 첨가하여 6 및 10일 간 39℃ 혐기적 조건에서 배양하여 메탄생성량을 조사하였다. BES, lumazaine과 ethyl 2-butynoate는 메탄생성균들의 메탄생성을 억제하였으며 첨가수준이 증가함으로써 억제력이 증가하였다. 그러나, BPS와 propynoic acid는 모든 메탄생성균의 메탄생성에 억제효과가 없었다. Methanosarcina 속의 균들이 BES, lumazaine 및 ethyl 2-butynoate에 대해 가장 저항성이 높았고, Methanobacteriales 목에 속하는 균들이 가장 작은 저항성을 나타내었다. 본 연구에서 이용된 화학물질들에 대한 메탄생성균들의 저항성은 메탄생성균들의 세포막구조의 차이에 의한 억제물질의 세포내 접근성, 억제물질의 메탄생성 pathway의 작용단계에 따른 기질이용성, H2의 분압의 변화에 따른 억제물질의 전자수용체로써 작용효과 등의 차이로 다양하였다. 결론적으로, 혼합 혐기 미생물 생태계에서 메탄생성균에 대해 메탄생성억제물질을 사용할 때 메탄생성억제효과의 차이를 충분히 고려해야할 것이다.
The objectives of the present study were to investigate the effect of five chemical inhibitors on methane production of the pure cultures of methanogens. To examine the methanogenesis inhibition by 2-bromoethane sulfonic acid (BES), 3-bromopropane sulfonic acid (BPS), lumazine, propynoic acid and e...
The objectives of the present study were to investigate the effect of five chemical inhibitors on methane production of the pure cultures of methanogens. To examine the methanogenesis inhibition by 2-bromoethane sulfonic acid (BES), 3-bromopropane sulfonic acid (BPS), lumazine, propynoic acid and ethyl 2-butynoate, they were added into the pure cultures of Methanobacterium formicicum KOR-1 and KOR-12, Methanoculleus bourgensis KOR-2, Methanosarcina mazei KOR-3, KOR-7, KOR-9 and KOR-10, Methanosarcina vacuolata (DSM 1232), Methanobacterium bryantii (ATCC 33272), Methanosarcina mazei (ATCC BAA159) and Methanosarcina barkeri (ATCC 43240). Each methanogen was anaerobically incubated at 39℃ for 6 and 10 days, and then methane gas production was measured. All methanogens were sensitive to BES, lumazaine and ethyl 2-butynoate and as the addition levels of the chemicals increased, the methanogenesis was linearly reduced. BPS and propynoic acid failed to inhibit any of the methanogens. There were important differences among species of methanogens regarding their sensitivity to the different inhibitors. In general, the strains of Methanosarcina species were the most resistant to BES, lumazaine and ethyl 2-butynoate and the strains of Methanobacteriales order the least resistant. The different resistances to chemical inhibitors may be caused by different cell membrane structure, substrate utilization for methanogenesis and competition with other microorganisms for electron receptors. In conclusion, differences among methanogens habituating in anaerobic ecosystems, such as the rumen of ruminants regarding their resistance to chemical inhibitors should be considered when strategies of inhibition of methanogenesis are designed.
The objectives of the present study were to investigate the effect of five chemical inhibitors on methane production of the pure cultures of methanogens. To examine the methanogenesis inhibition by 2-bromoethane sulfonic acid (BES), 3-bromopropane sulfonic acid (BPS), lumazine, propynoic acid and ethyl 2-butynoate, they were added into the pure cultures of Methanobacterium formicicum KOR-1 and KOR-12, Methanoculleus bourgensis KOR-2, Methanosarcina mazei KOR-3, KOR-7, KOR-9 and KOR-10, Methanosarcina vacuolata (DSM 1232), Methanobacterium bryantii (ATCC 33272), Methanosarcina mazei (ATCC BAA159) and Methanosarcina barkeri (ATCC 43240). Each methanogen was anaerobically incubated at 39℃ for 6 and 10 days, and then methane gas production was measured. All methanogens were sensitive to BES, lumazaine and ethyl 2-butynoate and as the addition levels of the chemicals increased, the methanogenesis was linearly reduced. BPS and propynoic acid failed to inhibit any of the methanogens. There were important differences among species of methanogens regarding their sensitivity to the different inhibitors. In general, the strains of Methanosarcina species were the most resistant to BES, lumazaine and ethyl 2-butynoate and the strains of Methanobacteriales order the least resistant. The different resistances to chemical inhibitors may be caused by different cell membrane structure, substrate utilization for methanogenesis and competition with other microorganisms for electron receptors. In conclusion, differences among methanogens habituating in anaerobic ecosystems, such as the rumen of ruminants regarding their resistance to chemical inhibitors should be considered when strategies of inhibition of methanogenesis are designed.
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