[논문]Interleukin-4-Mediated Oxidative Stress Is Harmful to Hippocampal Neurons of Prothrombin Kringle-2-Lesioned Rat In Vivo

Chung, Young Cheul; Jeong, Jae Yeong; Jin, Byung Kwan

doi:10.3390/antiox9111068

[해외논문] Interleukin-4-Mediated Oxidative Stress Is Harmful to Hippocampal Neurons of Prothrombin Kringle-2-Lesioned Rat In Vivo 원문보기

Antioxidants, v.9 no.11, 2020년, pp.1068 -

Chung, Young Cheul (Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Korea) , Jeong, Jae Yeong (youngcheul.chung@kitox.re.kr) , Jin, Byung Kwan (Department of Biochemistry & Molecular Biology, School of Medicine, Kyung Hee University, Seoul 02447, Korea)

Abstract ▼ AI-Helper

The present study investigated the effects of reactive microglia/macrophages-derived interleukin-4 (IL-4) on hippocampal neurons in prothrombin kringle-2 (pKr-2)-lesioned rats. pKr-2 was unilaterally injected into hippocampus in the absence or presence of IL-4 neutralizing antibody (IL-4Nab). Immunohistochemical analysis showed a significant loss of Nissl+ and NeuN+ cells and activation of microglia/macrophages (increase in reactive OX-42+ and OX-6+ cells) in the hippocampus at 7 days after pKr-2 injection. The levels of IL-4 expression were upregulated in the reactive OX-42+ microglia/macrophages as early as 1 day, maximal at 3 days and maintained up to 7 days after pKr-2 injection. Treatment with IL-4Nab significantly increased neuronal survival in pKr-2-treated CA1 layer of hippocampus in vivo. Accompanying neuroprotection, IL-4 neutralization inhibited activation of microglia/macrophages, reactive oxygen species-derived oxidative damages, production of myeloperoxidase- and inducible nitric oxide synthase-derived reactive nitrogen species and nitrosative damages as analyzed by immunohistochemistry and hydroethidine histochemistry. These results suggest that endogenous IL-4 expressed on reactive microglia/macrophages mediates oxidative/nitrosative stress and play a critical role on neurodegeneration of hippocampal CA1 layer in vivo.

Keyword

참고문헌 (39)

1. Cobb C.A. Cole M.P. Oxidative and nitrative stress in neurodegeneration Neurobiol. Dis. 2015 84 4 21 10.1016/j.nbd.2015.04.020 26024962

상세보기
2. Dasuri K. Zhang L. Keller J.N. Oxidative stress, neurodegeneration, and the balance of protein degradation and protein synthesis Free. Radic. Biol. Med. 2013 62 170 185 10.1016/j.freeradbiomed.2012.09.016 23000246

상세보기
3. Liochev S.I. Reactive oxygen species and the free radical theory of aging Free. Radic. Biol. Med. 2013 60 1 4 10.1016/j.freeradbiomed.2013.02.011 23434764

상세보기
4. Chung Y.C. Ko H.W. Bok E. Park E.S. Huh S.H. Nam J.H. Jin B.K. The role of neuroinflammation on the pathogenesis of Parkinson’s disease BMB Rep. 2010 43 225 232 10.5483/BMBRep.2010.43.4.225 20423606

원문보기 상세보기
5. Lin M.T. Beal M.F. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases Nat. Cell Biol. 2006 443 787 795 10.1038/nature05292 17051205

상세보기
6. Hickman S. Izzy S. Sen P. Morsett L. El Khoury J. Microglia in neurodegeneration Nat. Neurosci. 2018 21 1359 1369 10.1038/s41593-018-0242-x 30258234

상세보기
7. Wolf S.A. Boddeke H.W.G.M. Kettenmann H. Microglia in Physiology and Disease Annu. Rev. Physiol. 2017 79 619 643 10.1146/annurev-physiol-022516-034406 27959620

상세보기
8. Colonna M. Butovsky O. Microglia Function in the Central Nervous System During Health and Neurodegeneration Annu. Rev. Immunol. 2017 35 441 468 10.1146/annurev-immunol-051116-052358 28226226

상세보기
9. Jeong J.Y. Chung Y.C. Jin B.K. Interleukin-4 and Interleukin-13 Exacerbate Neurotoxicity of Prothrombin Kringle-2 in Cortex In Vivo via Oxidative Stress Int. J. Mol. Sci. 2019 20 1927 10.3390/ijms20081927

상세보기
10. Kim S.R. Chung E.S. Bok E. Baik H.H. Chung Y.C. Won S.Y. Joe E. Kim T.H. Kim S.S. Jin M.Y. Prothrombin kringle-2 induces death of mesencephalic dopaminergic neurons in vivo and in vitro via microglial activation J. Neurosci. Res. 2009 88 1537 1548 10.1002/jnr.22318

상세보기
11. Won S.Y. Choi S.H. Jin B.K. Prothrombin kringle-2-induced oxidative stress contributes to the death of cortical neurons in vivo and in vitro: Role of microglial NADPH oxidase J. Neuroimmunol. 2009 214 83 92 10.1016/j.jneuroim.2009.07.005 19660816

상세보기
12. Shin W.-H. Jeon M.-T. Leem E. Won S.-Y. Jeong K.H. Park S.-J. McLean C. Lee S.J. Jin B.K. Jung U.J. Induction of microglial toll-like receptor 4 by prothrombin kringle-2: A potential pathogenic mechanism in Parkinson’s disease Sci. Rep. 2015 5 14764 10.1038/srep14764 26440368

상세보기
13. Berzin T.M. Zipser B.D. Rafii M.S. Kuo-Leblanc V. Yancopoulo？ G.D. Glass D.J. Fallon J.R. Stopa E.G. Agrin and microvascular damage in Alzheimer’s disease Neurobiol. Aging 2000 21 349 355 10.1016/S0197-4580(00)00121-4 10867220

상세보기
14. Quarta A. Berneman Z. Ponsaerts P. Neuroprotective modulation of microglia effector functions following priming with interleukin 4 and 13: Current limitations in understanding their mode-of-action BrainBehav. Immun. 2020 88 856 866 10.1016/j.bbi.2020.03.023

상세보기
15. Sholl-Franco A. Da Silva A.G.L.S. Adao-Novaes J. Interleukin-4 as a Neuromodulatory Cytokine: Roles and signaling in the nervous system Ann. N. Y. Acad. Sci. 2009 1153 65 75 10.1111/j.1749-6632.2008.03962.x 19236329

상세보기
16. Ravelli K.G. Santos G.D. Dos Santos N.B. Munhoz C.D. Azzi-Nogueira D. Campos A.C. Pagano R.L. Britto L.R. Hernandes M.S. Nox2-dependent neuroinflammation in an EAE model of multiple sclerosis Transl. Neurosci. 2019 10 1 9 10.1515/tnsci-2019-0001 30984416

상세보기
17. Yang M.-S. Park E.J. Sohn S. Kwon H.J. Shin W.-H. Pyo H.K. Jin B. Choi K.S. Jou I. Joe E.-H. Interleukin-13 and -4 induce death of activated microglia Glia 2002 38 273 280 10.1002/glia.10057 12007140

상세보기
18. Nam J.H. Park K.W. Park E.S. Lee Y.B. Lee H.G. Baik H.H. Kim Y.S. Maeng S. Park J. Jin B.K. Interleukin-13/-4-induced oxidative stress contributes to death of hippocampal neurons in abeta1-42-treated hippocampus in vivo Antioxid. Redox Signal 2012 16 1369 1383 10.1089/ars.2011.4175 22248368

상세보기
19. Paxinos G. Watson C. The Rat Brain in Stereotaxic Coordinates Academic Press San Diego, CA, USA 1982
20. Baek J.Y. Jeong J.Y. Kim K.I. Won S.-Y. Chung Y.C. Nam J.H. Cho E.J. Ahn T.-B. Bok E. Shin W.-H. Inhibition of Microglia-Derived Oxidative Stress by Ciliary Neurotrophic Factor Protects Dopamine Neurons In Vivo from MPP+ Neurotoxicity Int. J. Mol. Sci. 2018 19 3543 10.3390/ijms19113543

상세보기
21. Choi S.H. Lee D.Y. Kim S.U. Jin B.K. Thrombin-Induced Oxidative Stress Contributes to the Death of Hippocampal Neurons In Vivo: Role of Microglial NADPH Oxidase J. Neurosci. 2005 25 4082 4090 10.1523/JNEUROSCI.4306-04.2005 15843610

상세보기
22. Park K.W. Baik H.H. Jin B.K. Interleukin-4-induced oxidative stress via microglial NADPH oxidase contributes to the death of hippocampal neurons in vivo Curr. Aging Sci. 2008 1 192 201 10.2174/1874609810801030192 20021392
23. Bok E. Cho E.J. Chung E.S. Shin W.-H. Jin B.K. Interleukin-4 Contributes to Degeneration of Dopamine Neurons in the Lipopolysaccharide-treated Substantia Nigra in vivo Exp. Neurobiol. 2018 27 309 319 10.5607/en.2018.27.4.309 30181693

상세보기
24. Bok E. Chung Y.C. Kim K.S. Baik H.H. Shin W.H. Jin B.K. Modulation of M1/M2 polarization by capsaicin contributes to the survival of dopaminergic neurons in the lipopolysaccharide-lesioned substantia nigra in vivo Exp. Mol. Med. 2018 50 1 14 10.1038/s12276-018-0111-4

상세보기
25. Ji K.-A. Yang M.-S. Jeong H.-K. Min K.-J. Kang S.-H. Jou I. Joe E.-H. Resident microglia die and infiltrated neutrophils and monocytes become major inflammatory cells in lipopolysaccharide-injected brain Glia 2007 55 1577 1588 10.1002/glia.20571 17823975

상세보기
26. Choi D.K. Pennathur S. Perier C. Tieu K. Teismann P. Wu D.C. Jackson-Lewis V. Vila M. Vonsattel J.P. Heinecke J.W. Ablation of the inflammatory enzyme myeloperoxidase mitigates features of Parkinson’s disease in mice J. Neurosci. 2005 25 6594 65600 10.1523/JNEUROSCI.0970-05.2005 16014720

상세보기
27. Tieu K. Ischiropoulos H. Przedborski S. Nitric oxide and reactive oxygen species in Parkinson’s disease IUBMB Life 2003 55 329 335 10.1080/1521654032000114320 12938735

상세보기
28. Jana M. Palencia C.A. Pahan K. Fibrillar amyloid-beta peptides activate microglia via TLR2: Implications for Alzheimer’s disease J. Immunol. 2008 181 7254 7262 10.4049/jimmunol.181.10.7254 18981147

상세보기
29. Jang J.-H. Lee S.H. Jung K. Yoo H.-R. Park G. Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation Brain Sci. 2020 10 32 10.3390/brainsci10010032

상세보기
30. Limon I.D. Diaz A. Mendieta L. Chamorro G. Espinosa B. Zenteno E. Guevara J. Amyloid-β25？35 impairs memory and increases NO in the temporal cortex of rats Neurosci. Res. 2009 63 129 137 10.1016/j.neures.2008.11.006 19084561

상세보기
31. Huh S.H. Chung Y.C. Piao Y. Jin M.Y. Son H.J. Yoon N.S. Hong J.Y. Pak Y.K. Kim Y.S. Hong J.K. Ethyl Pyruvate Rescues Nigrostriatal Dopaminergic Neurons by Regulating Glial Activation in a Mouse Model of Parkinson’s Disease J. Immunol. 2011 187 960 969 10.4049/jimmunol.1100009 21685323

상세보기
32. Marik C. Felts P.A. Bauer J. Lassmann H. Smith K.J. Lesion genesis in a subset of patients with multiple sclerosis: A role for innate immunity? Brain 2007 130 2800 2815 10.1093/brain/awm236 17956913

상세보기
33. Arnhold J. Flemmig J. Human myeloperoxidase in innate and acquired immunity Arch. Biochem. Biophys. 2010 500 92 106 10.1016/j.abb.2010.04.008 20399194

상세보기
34. Hampton M.B. Kettle A.J. Winterbourn C.C. Inside the neutrophil phagosome: Oxidants, myeloperoxidase, and bacterial killing Blood 1998 92 3007 3017 10.1182/blood.V92.9.3007 9787133

상세보기
35. Chung Y.C. Baek J.Y. Kim S.R. Ko H.W. Bok E. Shin W.-H. Won S.-Y. Jin B.K. Capsaicin prevents degeneration of dopamine neurons by inhibiting glial activation and oxidative stress in the MPTP model of Parkinson’s disease Exp. Mol. Med. 2017 49 e298 10.1038/emm.2016.159 28255166

상세보기
36. Lu N. Ding Y. Tian R. Peng Y. Inhibition of myeloperoxidase-mediated oxidative damage by nitrite in SH-SY5Y cells: Relevance to neuroprotection in neurodegenerative diseases Eur. J. Pharm. 2016 780 142 147 10.1016/j.ejphar.2016.03.042

상세보기
37. Genovese T. Esposito E. Mazzon E. Di Paola R. Meli R. Caminiti R. Bramanti P. Fink M.P. Cuzzocrea S. Beneficial effects of ethyl pyruvate in a mouse model of spinal cord injury Shock 2009 32 217 227 10.1097/SHK.0b013e31818d4073 18948848

상세보기
38. Orihuela R. McPherson C.A. Harry G.J. Microglial M1/M2 polarization and metabolic states Br. J. Pharmacol. 2016 173 649 665 10.1111/bph.13139 25800044

상세보기
39. Yao K. Zu H.-B. Microglial polarization: Novel therapeutic mechanism against Alzheimer’s disease Inflammopharmacology 2019 28 95 110 10.1007/s10787-019-00613-5 31264132

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

원문 보기

AccessON 원문보기

원문 URL 링크

DOI : 10.3390/antiox9111068 [무료]
PubMed Central : 저널 > 논문 [무료]
Molecular Diversity Preservation International : 저널
AccessON : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

오픈액세스(OA) 유형

GOLD

오픈액세스 학술지에 출판된 논문

저작권 관리 안내

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증