[논문]Polydeoxyribonucleotide Ameliorates Inflammation and Apoptosis in Achilles Tendon-Injury Rats

Rho, Jeong Ho; Ko, Il-Gyu; Jin, Jun-Jang; Hwang, Lakkyong; Kim, Sang-Hoon; Chung, Jun-Young; Hwang, Tae-Jun; Han, Jin Hee

doi:10.5213/inj.2040428.214

Polydeoxyribonucleotide Ameliorates Inflammation and Apoptosis in Achilles Tendon-Injury Rats 원문보기

International neurourology journal : INJ, v.24 suppl.2, 2020년, pp.79 - 87

Rho, Jeong Ho (Department of Anesthesiology and Pain Medicine, Okcheon St. Mary’s Hospital, Okcheon, Korea) , Ko, Il-Gyu (Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea) , Jin, Jun-Jang (Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea) , Hwang, Lakkyong (Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea) , Kim, Sang-Hoon (Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea) , Chung, Jun-Young (Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea) , Hwang, Tae-Jun (Department of Medicine, Graduate School, Kyung Hee University, Seoul, Korea) , Han, Jin Hee (Department of Anesthesiology and Pain Medicine, Kyung Hee University Medical Center, College of Medicine, Kyung Hee University, Seoul, Korea Korea<)

Abstract ▼ AI-Helper

PurposeAdenosine A2A receptor agonist polydeoxyribonucleotide (PDRN) possesses an anti-inflammatory effect and suppress apoptotic cell death in several disorders. In this current study, the effect of PDRN on inflammation and apoptosis in rats with Achilles tendon injury was investigated.Methodsvon Frey filament test and plantar test were conducted for the determination of pain threshold. Analysis of histological alterations was conducted by hematoxylin and eosin staining. Immunohistochemistry for cleaved caspase-3-positive cells and cleaved caspase-9-positive cells was done. Enzyme-linked immunoassay was used to detect the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and cyclic adenosine-3’,5’-monophosphate (cAMP). Western blot was conducted to detect the protein levels of cAMP response element-binding protein (CREB), protein kinase A (PKA), Bcl-2-associated X (Bax), and B-cell lymphoma 2 (Bcl-2).ResultsPDRN treatment relieved mechanical allodynia and alleviated thermal hyperalgesia after Achilles tendon injury. TNF-α and IL-6 concentrations were decreased by PDRN application. PDRN injection significantly enhanced cAMP concentration and phosphorylated CREB versus CREB ratio, showing cAMP-PKA-CREB pathway was activated by PDRN application. PDRN treatment inhibited percentages of cleaved caspase-3-positive cells and caspase-9-posiive cells and the suppressed Bax versus Bcl-2 ratio in Achilles tendon injury rats.ConclusionsPDRN is probably believed to have a good effect on pain and inflammation in the urogenital organs. PDRN may be used as a new treatment for Achilles tendon injury.

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참고문헌 (27)

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Polydeoxyribonucleotide Ameliorates Inflammation and Apoptosis in Achilles Tendon-Injury Rats 원문보기

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