[논문]Clmp Regulates AMPA and Kainate Receptor Responses in the Neonatal Hippocampal CA3 and Kainate Seizure Susceptibility in Mice

Jang, Seil; Yang, Esther; Kim, Doyoun; Kim, Hyun; Kim, Eunjoon

doi:10.3389/fnsyn.2020.567075

[해외논문] Clmp Regulates AMPA and Kainate Receptor Responses in the Neonatal Hippocampal CA3 and Kainate Seizure Susceptibility in Mice 원문보기

Frontiers in synaptic neuroscience, v.12, 2020년, pp.567075 -

Jang, Seil (Center for Synaptic Brain Dysfunctions, Institute for Basic Science , Daejeon , South Korea) , Yang, Esther (Department of Anatomy and Division of Brain Korea 21, Biomedical Science, College of Medicine, Korea University , Seoul , South Korea) , Kim, Doyoun (Center for Drug Discovery Platform Research, Korea Research Institute of Chemical Technology (KRICT) , Daejeon , South Korea) , Kim, Hyun (Department of Anatomy and Division of Brain Korea 21, Biomedical Science, College of Medicine, Korea University , Seoul , South Korea) , Kim, Eunjoon (Center for Synaptic Brain Dysfunctions, Institute for Basic Science , Daejeon , South Korea)

Abstract ▼ AI-Helper

Synaptic adhesion molecules regulate synapse development through trans-synaptic adhesion and assembly of diverse synaptic proteins. Many synaptic adhesion molecules positively regulate synapse development; some, however, exert negative regulation, although such cases are relatively rare. In addition, synaptic adhesion molecules regulate the amplitude of post-synaptic receptor responses, but whether adhesion molecules can regulate the kinetic properties of post-synaptic receptors remains unclear. Here we report that Clmp, a homophilic adhesion molecule of the Ig domain superfamily that is abundantly expressed in the brain, reaches peak expression at a neonatal stage (week 1) and associates with subunits of AMPA receptors (AMPARs) and kainate receptors (KARs). Clmp deletion in mice increased the frequency and amplitude of AMPAR-mediated miniature excitatory post-synaptic currents (mEPSCs) and the frequency, amplitude, and decay time constant of KAR-mediated mEPSCs in hippocampal CA3 neurons. Clmp deletion had minimal impacts on evoked excitatory synaptic currents at mossy fiber-CA3 synapses but increased extrasynaptic KAR, but not AMPAR, currents, suggesting that Clmp distinctly inhibits AMPAR and KAR responses. Behaviorally, Clmp deletion enhanced novel object recognition and susceptibility to kainate-induced seizures, without affecting contextual or auditory cued fear conditioning or pattern completion-based contextual fear conditioning. These results suggest that Clmp negatively regulates hippocampal excitatory synapse development and AMPAR and KAR responses in the neonatal hippocampal CA3 as well as object recognition and kainate seizure susceptibility in mice.

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해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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