[논문]All-in-one pumpless portable genetic analysis microsystem for rapid naked-eye detection

Choi, Yunho; Song, Younseong; Kim, Yong Tae; Kim, Hogi; Park, Yoo Min; Lee, Seok Jae; Im, Sung Gap; Lee, Kyoung G.

doi:10.1016/j.snb.2021.130307

[해외논문] All-in-one pumpless portable genetic analysis microsystem for rapid naked-eye detection

Sensors and actuators. B, Chemical, v.344, 2021년, pp.130307 -

Choi, Yunho (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Song, Younseong (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Kim, Yong Tae (Department of Chemical Engineering & Biotechnology, Korea Polytechnic University) , Kim, Hogi (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Park, Yoo Min (Nano-bio Application Team, National Nanofab Center) , Lee, Seok Jae (Nano-bio Application Team, National Nanofab Center) , Im, Sung Gap (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) , Lee, Kyoung G. (Nano-bio Application Team, National Nanofab Center)

Abstract ▼ AI-Helper

Abstract Point-of-care testing (POCT) is highly desirable for early detection of infectious diseases; however, current molecular diagnostics require a series of sophisticated facilities and instruments, which critically limits its direct application to POCT. From this perspective, chip-scale microfluidic technologies have been widely investigated to provide rapid, low-cost POCT diagnostics. Despite the development of small-footprint portable microfluidic chips for molecular diagnostics, the integration of functional steps into POCT is still a crucial, unsolved issue to be addressed. Herein, we suggest an all-in-one pumpless portable genetic analysis microsystem for molecular diagnostics with facile and portable operation by suggesting three main functions: (i) a finger-powered actuator for exquisite fluid control, (ii) a poly(dimethylaminomethyl styrene)-based polymeric surface for efficient nucleic acid harvest and extraction, and (iii) loop-mediated isothermal amplification-based amplification and colorimetric detection. The developed system can be stored at room temperature, shows a wide detection range from 1 × 103 to 1 × 107 cells, and enables naked-eye detection with a total detection time of less than 80 min. This all-in-one pumpless portable genetic analysis microsystem can be utilized as a simple and accurate platform for POCT diagnostics. Highlights Fabrication and evaluation of an all-in-one instrument-free portable microsystem for foodborne pathogen detection. Finger-powered actuation, DNA extraction, isothermal amplification and naked-eye detection on the developed microsystem. Naked-eye detection of Escherichia coli O157:H7 and Staphylococcus aureus.

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