[논문]Spread spectrum SERS allows label-free detection of attomolar neurotransmitters

Lee, Wonkyoung; Kang, Byoung-Hoon; Yang, Hyunwoo; Park, Moonseong; Kwak, Ji Hyun; Chung, Taerin; Jeong, Yong; Kim, Bong Kyu; Jeong, Ki-Hun

doi:10.1038/s41467-020-20413-8

[해외논문] Spread spectrum SERS allows label-free detection of attomolar neurotransmitters 원문보기

Nature communications, v.12 no.1, 2021년, pp.159 -

Lee, Wonkyoung (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea) , Kang, Byoung-Hoon (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea) , Yang, Hyunwoo (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea) , Park, Moonseong (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea) , Kwak, Ji Hyun (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea) , Chung, Taerin (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea) , Jeong, Yong (Department) , Kim, Bong Kyu , Jeong, Ki-Hun

Abstract ▼ AI-Helper

The quantitative label-free detection of neurotransmitters provides critical clues in understanding neurological functions or disorders. However, the identification of neurotransmitters remains challenging for surface-enhanced Raman spectroscopy (SERS) due to the presence of noise. Here, we report spread spectrum SERS (ss-SERS) detection for the rapid quantification of neurotransmitters at the attomolar level by encoding excited light and decoding SERS signals with peak autocorrelation and near-zero cross-correlation. Compared to conventional SERS measurements, the experimental result of ss-SERS shows an exceptional improvement in the signal-to-noise ratio of more than three orders of magnitude, thus achieving a high temporal resolution of over one hundred times. The ss-SERS measurement further allows the attomolar SERS detection of dopamine, serotonin, acetylcholine, γ-aminobutyric acid, and glutamate without Raman reporters. This approach opens up opportunities not only for investigating the early diagnostics of neurological disorders or highly sensitive biomedical SERS applications but also for developing low-cost spectroscopic biosensing applications.Identification of neurotransmitters remains challenging for surface enhanced Raman spectroscopy (SERS) due to presence of noise. Here, the authors present spread spectrum SERS, which by encoding excited light and decoding SERS signals enables detection of unlabelled neurotransmitters at attomolar concentrations.

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