[논문]Single-cell RNA sequencing of human nail unit defines RSPO4 onychofibroblasts and SPINK6 nail epithelium

Kim, Hyun Je; Shim, Joon Ho; Park, Ji-Hye; Shin, Hyun Tae; Shim, Jong Sup; Jang, Kee-Taek; Park, Woong-Yang; Lee, Kyung-Hoon; Kwon, Eun Ji; Jang, Hyung-Suk; Yang, Hanseul; Lee, Jong Hee; Yang, Jun-Mo; Lee, Dongyoun

doi:10.1038/s42003-021-02223-w

[해외논문] Single-cell RNA sequencing of human nail unit defines RSPO4 onychofibroblasts and SPINK6 nail epithelium 원문보기

Communications biology, v.4 no.1, 2021년, pp.692 -

Kim, Hyun Je (Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea) , Shim, Joon Ho (Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea) , Park, Ji-Hye (Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea) , Shin, Hyun Tae (Department of Dermatology, Inha University School of Medicine, Incheon, Republic of Korea) , Shim, Jong Sup (Department of Orthopedic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea) , Jang, Kee-Taek (Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea) , Park, Woong-Yang (Samsung Genomic Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea) , Lee, Kyung-Hoon (Department of Anatomy, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea) , Kwon, Eun Ji (Morristown Pathology Associates, Morristown, NJ USA) , Jang, Hyung-Suk (Department of Dermatolo) , Yang, Hanseul , Lee, Jong Hee , Yang, Jun-Mo , Lee, Dongyoun

Abstract ▼ AI-Helper

Research on human nail tissue has been limited by the restricted access to fresh specimen. Here, we studied transcriptome profiles of human nail units using polydactyly specimens. Single-cell RNAseq with 11,541 cells from 4 extra digits revealed nail-specific mesenchymal and epithelial cell populations, characterized by RSPO4 (major gene in congenital anonychia) and SPINK6, respectively. In situ RNA hybridization demonstrated the localization of RSPO4, MSX1 and WIF1 in onychofibroblasts suggesting the activation of WNT signaling. BMP-5 was also expressed in onychofibroblasts implicating the contribution of BMP signaling. SPINK6 expression distinguished the nail-specific keratinocytes from epidermal keratinocytes. RSPO4+ onychofibroblasts were distributed at close proximity with LGR6+ nail matrix, leading to WNT/β-catenin activation. In addition, we demonstrated RSPO4 was overexpressed in the fibroblasts of onychomatricoma and LGR6 was highly expressed at the basal layer of the overlying epithelial component, suggesting that onychofibroblasts may play an important role in the pathogenesis of onychomatricoma.Kim et al. conducted single-cell RNA sequencing to determine the transcriptome profiles of human nail units using polydactyly specimens to demonstrate mesenchymal and epithelial cell populations, characterized by RSPO4 and SPINK6 respectively. RSPO4 + onychofibroblasts localized with LGR6 + nail matrix, leading to WNT/ β-catenin activation and suggesting a role for onychofibroblasts in onychomatricoma pathogenesis.

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