[논문]Drug Evaluation Based on a Multi-Channel Cell Chip with a Horizontal Co-Culture

Kim, Gyeong-Ji; Lee, Kwon-Jai; Choi, Jeong-Woo; An, Jeung Hee

doi:10.3390/ijms22136997

[해외논문] Drug Evaluation Based on a Multi-Channel Cell Chip with a Horizontal Co-Culture 원문보기

International journal of molecular sciences, v.22 no.13, 2021년, pp.6997 -

Kim, Gyeong-Ji (Department of Food and Nutrition, KC University, Seoul 07661, Korea) , Lee, Kwon-Jai (kgj8495@hanmail.net) , Choi, Jeong-Woo (College of H-LAC, Daejeon University, Daejeon 34520, Korea) , An, Jeung Hee (kjlee@dju.kr)

Abstract ▼ AI-Helper

We developed a multi-channel cell chip containing a three-dimensional (3D) scaffold for horizontal co-culture and drug toxicity screening in multi-organ culture (human glioblastoma, cervical cancer, normal liver cells, and normal lung cells). The polydimethylsiloxane (PDMS) multi-channel cell chip (PMCCC) was based on fused deposition modeling (FDM) technology. The architecture of the PMCCC was an open-type cell chip and did not require a pump or syringe. We investigated cell proliferation and cytotoxicity by conducting 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays and analysis of oleanolic acid (OA)-treated multi-channel cell chips. The results of the MTT and LDH assays showed that OA treatment in the multi-channel cell chip of four cell lines enhanced chemoresistance of cells compared with that in the 2D culture. Furthermore, we demonstrated the feasibility of the application of our multi-channel cell chip in various analysis methods through Annexin V-fluorescein isothiocyanate/propidium iodide staining, which is not used for conventional cell chips. Taken together, the results demonstrated that the PMCCC may be used as a new 3D platform because it enables simultaneous drug screening in multiple cells by single point injection and allows analysis of various biological processes.

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