[논문]Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

Woo, Hyun-A; Ku, Seong Sub; Jie, Eun Yee; Kim, HyeRan; Kim, Hyun-Soon; Cho, Hye Sun; Jeong, Won-Joong; Park, Sang Un; Min, Sung Ran; Kim, Suk Weon

doi:10.1038/s41598-021-94597-4

[해외논문] Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus 원문보기

Scientific reports, v.11 no.1, 2021년, pp.15120 -

Woo, Hyun-A (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Ku, Seong Sub (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Jie, Eun Yee (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup‑) , Kim, HyeRan (si, Jeollabuk‑) , Kim, Hyun-Soon (do 56212 Republic of Korea) , Cho, Hye Sun (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Jeong, Won-Joong (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Park, Sang Un (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea) , Min, Sung Ran (Plant Systems Engin) , Kim, Suk Weon

Abstract ▼ AI-Helper

To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.

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