[논문]Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars

Jalaludin, Iqbal; Kim, Jeongkwon

doi:10.1016/j.foodchem.2021.130514

[해외논문] Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars

Food chemistry, v.365, 2021년, pp.130514 -

Jalaludin, Iqbal (Department of Chemistry, Chungnam National University) , Kim, Jeongkwon (Department of Chemistry, Chungnam National University)

Abstract ▼ AI-Helper

Abstract Refractive index (RI) detection is the standard approach for quantitatively detecting sugars via high-performance liquid chromatography (HPLC), while ultraviolet (UV) absorbance detection is the most commonly used detection method for general HPLC analysis. We compared the two detection approaches of UV and RI in the HPLC analysis of small sugars to investigate whether UV detection could be an alternative method to RI detection. UV detection was performed using a photodiode array scanning from 190 to 400 nm. We obtained comparable limit of detection (LOD) results for RI and UV detection in the HPLC analysis of monosaccharides, while HPLC-RI provided better LOD results than HPLC-UV in disaccharide analysis. Both HPLC-RI and HPLC-UV methods were applied to analyze a real honey sample, and similar results were obtained in terms of precision and recovery. The study conclusively shows that the UV-based HPLC analysis of sugars offers a sufficient alternative to RI-based HPLC analysis. Highlights HPLC-UV method is comparable to HPLC-RI method for sensitive sugar analysis. HPLC-UV method with gradient elution enables sugar analysis time to be shortened. Both methods are appropriate for sugar determination in honey.

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참고문헌 (27)

Plant Foods for Human Nutrition Barreira 65 1 38 2010 10.1007/s11130-009-0147-7 Sugars profiles of different chestnut (Castanea sativa Mill.) and almond (Prunus dulcis) cultivars by HPLC-RI

상세보기
BeMiller 333 2017 Food analysis Carbohydrate analysis
Journal of Chromatography A Chávez-Servín 1043 2 211 2004 10.1016/j.chroma.2004.06.002 Analysis of mono- and disaccharides in milk-based formulae by high-performance liquid chromatography with refractive index detection

상세보기
Food Chemistry De La Fuente 129 4 1483 2011 10.1016/j.foodchem.2011.05.121 Carbohydrate composition of Spanish unifloral honeys

상세보기
10.1016/j.microc.2016.12.004 Ferreira, S. L. C., Caires, A. O., Borges, T. da S., Lima, A. M. D. S., Silva, L. O. B., & dos Santos, W. N. L. (2017). Robustness evaluation in analytical methods optimized using experimental designs. Microchemical Journal, 131(October 2017), 163-169. 10.1016/j.microc.2016.12.004.

상세보기
Food Safety Korea. (2020). 6.12.1 Honey. Retrieved November 17, 2020, from https://www.foodsafetykorea.go.kr/foodcode/01_03.jsp?idx=11167.
Harris, D. C. (2003). Introduction to analytical separations. In Quantitative chemical analysis (6th ed.). W. H. Freeman. Retrieved from https://books.google.co.kr/books?id=csTsQr-v0d0C.
Review of Scientific Instruments Higashi 78 10 103107 2007 10.1063/1.2796928 An attenuated total reflectance far-UV spectrometer

상세보기
Journal of Chromatographic Science Hirata 34 1 58 1996 10.1093/chromsci/34.1.58 Refractive index detection using an ultraviolet detector with a capillary flow cell in preparative SFC

상세보기
Analytical Biochemistry Honda 180 2 351 1989 10.1016/0003-2697(89)90444-2 High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl5-pyrazolone derivatives

상세보기
ICH. (1995). ICH topic Q2 (R1) validation of analytical procedures: Text and methodology. Retrieved November 10, 2020, from https://www.ema.europa.eu/en/documents/scientific-guideline/ich-q-2-r1-validation-analytical-procedures-text-methodology-step-5_en.pdf.
Saudi Journal of Biological Sciences Kamal 18 1 17 2011 10.1016/j.sjbs.2010.09.003 Determination of sugars in honey by liquid chromatography

상세보기
Journal of Chromatographic Science Karkacier 41 6 331 2003 10.1093/chromsci/41.6.331 Comparison of different extraction and detection methods for sugars using amino-bonded phase HPLC

상세보기
Food Chemistry Lee 342 128356 2021 10.1016/j.foodchem.2020.128356 MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix

상세보기
Food Chemistry Ma 145 784 2014 10.1016/j.foodchem.2013.08.135 Simultaneous separation and determination of fructose, sorbitol, glucose and sucrose in fruits by HPLC-ELSD

상세보기
Journal of the American Chemical Society Pickett 73 10 4865 1951 10.1021/ja01154a117 The Vacuum Ultraviolet Absorption Spectra of Cyclic Compounds. II. Tetrahydrofuran, Tetrahydropyran, 1,4-Dioxane and Furan

상세보기
Rao 2018 Calibration and Validation of Analytical Methods - A Sampling of Current Approaches
10.1016/S0301-4770(08)70046-0 Scott, R. P. W. (Ed.). (1986). Chapter 8 The selection of the appropriate detector. In Liquid chromatography detectors (Vol. 33, pp. 235-261). Elsevier. 10.1016/S0301-4770(08)70046-0.
Journal of Food Composition and Analysis Se 80 February 16 2019 10.1016/j.jfca.2019.04.001 Detection techniques for adulterants in honey: Challenges and recent trends

상세보기
Journal of the Science of Food and Agriculture Shaw 34 1 109 1983 10.1002/jsfa.2740340116 Separation of fructose, glucose and sucrose in fruit by high performance liquid chromatography using UV detection at 190 nm

상세보기
Journal of Liquid Chromatography and Related Technologies Swartz 33 9-12 1130 2010 10.1080/10826076.2010.484356 HPLC detectors: A brief review

상세보기
Applied Spectroscopy Tachibana 65 2 221 2011 10.1366/10-06036 Far-ultraviolet spectra of n-alkanes and branched alkanes in the liquid phase observed using an attenuated total reflection-far ultraviolet (ATR-FUV) spectrometer

상세보기
Agronomy Research Tihomirova 14 5 1713 2016 Application of conventional HPLC RI technique for sugar analysis in hydrolysed hay
Journal of Chromatography A Uchiho 1424 86 2015 10.1016/j.chroma.2015.11.006 Far-ultraviolet absorbance detection of sugars and peptides by high-performance liquid chromatography

상세보기
Journal of Pharmaceutical and Biomedical Analysis Vander Heyden 24 5-6 723 2001 10.1016/S0731-7085(00)00529-X Guidance for robustness/ruggedness tests in method validation

상세보기
Food Chemistry Wang 172 669 2015 10.1016/j.foodchem.2014.09.044 Detection of honey adulteration with starch syrup by high performance liquid chromatography

상세보기
Analytical and Bioanalytical Chemistry Zhang 390 1 299 2008 10.1007/s00216-007-1633-0 Advances in HPLC detection-towards universal detection

상세보기

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[해외논문] Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars

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[해외논문] Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars

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