[논문]Octopromycin: Antibacterial and antibiofilm functions of a novel peptide derived from Octopus minor against multidrug-resistant Acinetobacter baumannii

Rajapaksha, D.C.; Jayathilaka, E.H.T. Thulshan; Edirisinghe, S.L.; Nikapitiya, Chamilani; Lee, Joeun; Whang, Ilson; De Zoysa, Mahanama

doi:10.1016/j.fsi.2021.07.019

[해외논문] Octopromycin: Antibacterial and antibiofilm functions of a novel peptide derived from Octopus minor against multidrug-resistant Acinetobacter baumannii

Fish & shellfish immunology, v.117, 2021년, pp.82 - 94

Rajapaksha, D.C. (College of Veterinary Medicine, Chungnam National University) , Jayathilaka, E.H.T. Thulshan (College of Veterinary Medicine, Chungnam National University) , Edirisinghe, S.L. (College of Veterinary Medicine, Chungnam National University) , Nikapitiya, Chamilani (College of Veterinary Medicine, Chungnam National University) , Lee, Joeun (National Marine Biodiversity Institute of Korea (MABIK)) , Whang, Ilson (National Marine Biodiversity Institute of Korea (MABIK)) , De Zoysa, Mahanama (College of Veterinary Medicine, Chungnam National University)

Abstract ▼ AI-Helper

Abstract The emergence of carbapenem-resistant Acinetobacter baumannii has increased the risk of nosocomial infections, which pose a huge health threat. There is an urgent need to develop alternative therapies, including broad-spectrum antimicrobial peptides. In this study, we designed, characterized, and studied the antibacterial, antibiofilm effects and possible mode of actions of a novel synthetic peptide Octopromycin, derived from the proline-rich protein 5 of Octopus minor. Octopromycin consists of 38 amino acids, (+5) net positive charge, high hydrophobic residue ratio (36%), and two α-helix secondary structures. The minimum inhibitory concentration and minimum bactericidal concentration against A. baumannii were 50 and 200 μg/mL, respectively. Time-kill kinetics and bacterial viability assays confirmed the concentration-dependent antibacterial activity of Octopromycin. Field emission scanning electron microscopy images clearly showed ultrastructural alterations in Octopromycin-treated A. baumannii cells. Propidium iodide penetrated into Octopromycin-treated A. baumannii cells, demonstrating the loss of cell membrane integrity. Octopromycin treatment increased the production of reactive oxygen species in a concentration-dependent manner, and it inhibited the biofilm formation and showed biofilm eradication activity against A. baumannii. In vitro and in vivo safety evaluation revealed that Octopromycin was nontoxic to HEK293T and Raw 264.7 cells (<400 μg/mL), as well as mice red blood cells (<300 μg/mL), and zebrafish embryos (<4 μg/mL). An in vivo study results revealed that the A. baumannii-infected fish treated with Octopromycin exhibited a significantly higher relative percent survival (37.5%) than the infected mock-treated fish with PBS (16.6%). Furthermore, a decreased bacterial load and fewer alterations in histological analysis confirmed the successful control of A. baumannii by Octopromycin in vivo. Collectively, the results indicate that the antibacterial peptide Octopromycin may achieve rapid control of A. baumannii through multi-target interactions; it presents a desirable therapeutic option for the prevention and control of the infections. Highlights Antimicrobial peptide “Octopromycin is derived from prolin rich protein from Octopus minor. Octopromycin displayed strong antibacterial activity against Acinetobacter baumannii. Octopromycin can inhibit and eradicate the biofilm associated with A. baumannii. A. baumannii infected zebrafish are protected by Octopromycin treatment. Graphical abstract [DISPLAY OMISSION]

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참고문헌 (46)

Am. J. Transl. Res. Lei 11 3919 2019 The antimicrobial peptides and their potential clinical applications
Front. Cell. Infect. Microbiol. Mahlapuu 6 1 2016 10.3389/fcimb.2016.00194 Antimicrobial peptides: an emerging category of therapeutic agents

상세보기
Front. Pharmacol. Pfalzgraff 9 1 2018 10.3389/fphar.2018.00281 Antimicrobial peptides and their therapeutic potential for bacterial skin infections and wounds

상세보기
Int. J. Pept. Res. Therapeut. Bertrand 25 1441 2019 10.1007/s10989-018-9789-3 Marine Antimicrobial Peptides: a promising source of new generation antibiotics and other bio-active molecules

상세보기
Marine Research Institute, Tuticorin Monolisha 4 3582 2013 Molecular characterization and antimicrobial activity of Octopus aegina and Octopus dolfusii in gulf of mannar coast
Mar. Drugs Maselli 18 2020 10.3390/md18080380 OctoPartenopin: identification and preliminary characterization of a novel antimicrobial peptide from the suckers of Octopus vulgaris

상세보기
Mar. Drugs Nikapitiya 18 2020 10.3390/md18010056 Octominin: a novel synthetic anticandidal peptide derived from defense protein of Octopus minor

상세보기
Fish Shellfish Immunol. Jayathilaka 110 23 2020 10.1016/j.fsi.2020.12.017 Octominin: an antibacterial and anti-biofilm peptide for controlling the multidrug resistance and pathogenic Streptococcus parauberis

상세보기
Mol. Sci Jayathilaka 2021 10.3390/ijms22105353 Antimicrobial and anti-biofilm peptide Octominin for controlling multidrug-resistant Acinetobacter baumannii

상세보기
Open Forum Infect. Dis. Marturano 6 1 2019 10.1093/ofid/ofz503 ESKAPE pathogens in bloodstream infections are associated with higher cost and mortality but can be predicted using diagnoses upon admission

상세보기
J. Clin. Outcome Manag. Alsan 17 27 2010 Acinetobacter baumannii: an emerging and important pathogen
Bull. Vet. Inst. Pulawy Kozińska 58 193 2014 10.2478/bvip-2014-0029 Acinetobacter johnsonii and Acinetobacter lwoffii : the emerging fish pathogens

상세보기
Aquaculture Behera 471 157 2017 10.1016/j.aquaculture.2017.01.018 Molecular characterization and pathogenicity of a virulent Acinetobacter baumannii associated with mortality of farmed Indian major Carp Labeo rohita (Hamilton 1822)

상세보기
Curr. Sci. Rauta 101 548 2011 Emerging new multi-drug resistant bacterial pathogen, Acinetobacter baumannii associated with snakehead Channa striatus eye infection
PloS One Luna 14 1 2019 10.1371/journal.pone.0219824 Natural history of Acinetobacter baumannii infection in mice

상세보기
Ann. Clin. Microbiol. Antimicrob. Raible 16 1 2017 10.1186/s12941-017-0248-3 Molecular characterization of β-lactamase genes in clinical isolates of carbapenem-resistant Acinetobacter baumannii

상세보기
PloS One Majumder 14 1 2019 10.1371/journal.pone.0219693 Computational screening of antimicrobial peptides for Acinetobacter baumannii

상세보기
Sci. Adv. Nagarajan 5 2019 10.1126/sciadv.aax1946 W76: a designed antimicrobial peptide to combat carbapenem- and tigecycline-resistant Acinetobacter baumannii

상세보기
J. Biol. Chem. Lee 278 7927 2003 10.1074/jbc.M209239200 Processing of an antibacterial peptide from hemocyanin of the freshwater crayfish Pacifastacus leniusculus

상세보기
Dev. Comp. Immunol. Noga 35 409 2011 10.1016/j.dci.2010.11.015 Primary structure and cellular localization of callinectin, an antimicrobial peptide from the blue crab

상세보기
Nucleic Acids Res. Wang 44 D1087 2016 10.1093/nar/gkv1278 APD3: the antimicrobial peptide database as a tool for research and education

상세보기
Sci. Rep. Klubthawee 10 1 2020 10.1038/s41598-020-65688-5 A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa

상세보기
PloS One Jantaruk 12 1 2017 10.1371/journal.pone.0183852 Potential role of an antimicrobial peptide, KLK in inhibiting lipopolysaccharide-induced macrophage inflammation

상세보기
Biochim. Biophys. Acta Biomembr. Belokoneva 1617 22 2003 10.1016/j.bbamem.2003.08.010 The hemolytic activity of six arachnid cationic peptides is affected by the phosphatidylcholine-to-sphingomyelin ratio in lipid bilayers

상세보기
Fish Shellfish Immunol. Edirisinghe 94 558 2019 10.1016/j.fsi.2019.09.054 Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila

상세보기
Mar. Drugs Rajapaksha 18 2020 10.3390/md18110556 Spirulina maxima derived pectin nanoparticles enhance the immunomodulation, stress tolerance, and wound healing in zebrafish

상세보기
Int. J. Antimicrob. Agents Silva 41 2013 10.1016/j.ijantimicag.2012.07.020 The importance of antimicrobial peptides and their potential for therapeutic use in ophthalmology

상세보기
Int. J. Mol. Sci. Kim 19 2018 10.3390/ijms19103041 Antibacterial and antibiofilm activity and mode of action of magainin 2 against drug-resistant acinetobacter baumannii

상세보기
Biochim. Biophys. Acta Biomembr. Zelezetsky 1758 1436 2006 10.1016/j.bbamem.2006.03.021 Alpha-helical antimicrobial peptides-Using a sequence template to guide structure-activity relationship studies

상세보기
Galloux
Proc. Natl. Acad. Sci. U.S.A. Mwangi 116 26516 2019 10.1073/pnas.1909585117 The antimicrobial peptide ZY4 combats multidrugresistant Pseudomonas aeruginosa and Acinetobacter baumannii infection

상세보기
Infect. Drug Resist. Peng 12 2417 2019 10.2147/IDR.S214057 Antibacterial mechanism of peptide Cec4 against Acinetobacter baumannii
J. Antimicrob. Chemother. Gonzalez 68 2291 2013 Influence of the MBC/MIC ratio on the antibacterial activity of vancomycin versus linezolid against methicillin-resistant Staphylococcus aureus isolates in a pharmacodynamic model simulating serum and soft tissue interstitial fluid concentrations reported
Int. J. Antimicrob. Agents Lai 42 141 2013 10.1016/j.ijantimicag.2013.04.022 Identification and characterisation of the putative phage-related endolysins through full genome sequence analysis in Acinetobacter baumannii ATCC 17978

상세보기
Exp. Ther. Med. Li 10 1768 2015 10.3892/etm.2015.2731 Anti-candidal activity of a novel peptide derived from human chromogranin A and its mechanism of action against Candida krusei

상세보기
Curr. Opin. Microbiol. Zhao 21 1 2014 10.1016/j.mib.2014.06.008 Reactive oxygen species and the bacterial response to lethal stress

상세보기
Sci. Rep. Sato 9 2 2019 10.1038/s41598-019-53846-3 Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages

상세보기
Virulence Roy 9 522 2018 10.1080/21505594.2017.1313372 Strategies for combating bacterial biofilms: a focus on anti-biofilm agents and their mechanisms of action

상세보기
Pediatr. Pulmonol. Bjarnsholt 44 547 2009 10.1002/ppul.21011 Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients

상세보기
Future Microbiol. Gaddy 4 273 2009 10.2217/fmb.09.5 Regulation of Acinetobacter baumannii biofilm formation

상세보기
Biochem. J. Zarko 468 2 259 2015 10.1042/BJ20141251 Mechanisms of biofilm inhibition and degradation by antimicrobial peptides

상세보기
PloS One Kishi 13 1 2018 Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus
Int. J. Pept. Res. Therapeut. Bacalum 21 47 2015 10.1007/s10989-014-9430-z Cationic antimicrobial peptides cytotoxicity on mammalian cells: an analysis using therapeutic index integrative concept

상세보기
Aquaculture Xia 284 285 2008 10.1016/j.aquaculture.2008.07.038 Recovery of Acinetobacter baumannii from diseased channel catfish (Ictalurus punctatus) in China

상세보기
Proc. Natl. Acad. Sci. U.S.A. Bhuiyan 113 9599 2016 10.1073/pnas.1523116113 Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis

상세보기
Emerg. Dis. Cao 65 1816 2018 10.1111/tbed.12957 Acinetobacter lwoffii, an emerging pathogen for fish in Schizothorax genus in China, Transbound

상세보기

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