[논문]SARS-CoV-2 E Gene Variant Alters Analytical Sensitivity Characteristics of Viral Detection Using a Commercial Reverse Transcription-PCR Assay

Tahan, Stephen; Parikh, Bijal A.; Droit, Lindsay; Wallace, Meghan A.; Burnham, Carey-Ann D.; Wang, David; Tang, Yi-Wei

doi:10.1128/jcm.00075-21

[해외논문] SARS-CoV-2 E Gene Variant Alters Analytical Sensitivity Characteristics of Viral Detection Using a Commercial Reverse Transcription-PCR Assay 원문보기

Journal of clinical microbiology, v.59 no.7, 2021년, pp.e00075-21 -

Tahan, Stephen (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA) , Parikh, Bijal A. (Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA) , Droit, Lindsay (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA) , Wallace, Meghan A. (Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA) , Burnham, Carey-Ann D. (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA) , Wang, David (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA) , Tang, Yi-Wei

Abstract ▼ AI-Helper

Diagnostic assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential for patient management, infection prevention, and the public health response for coronavirus disease 2019 (COVID-19). The efficacy and reliability of these assays are of paramount importance in both tracking and controlling the spread of the virus.ABSTRACTDiagnostic assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential for patient management, infection prevention, and the public health response for coronavirus disease 2019 (COVID-19). The efficacy and reliability of these assays are of paramount importance in both tracking and controlling the spread of the virus. Real-time reverse transcription-PCR (RT-PCR) assays rely on a fixed genetic sequence for primer and probe binding. Mutations can potentially alter the accuracy of these assays and lead to unpredictable analytical performance characteristics and false-negative results. Here, we identify a G-to-U transversion (nucleotide 26372) in the SARS-CoV-2 E gene in three specimens with reduced viral detection efficiency using a widely available commercial assay. Further analysis of the public GISAID repository led to the identification of 18 additional genomes with this mutation, which reflect five independent mutational events. This work supports the use of dual-target assays to reduce the number of false-negative PCR results.

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